Abstract：
:Evolution by natural selection occurs in cultures of Escherichia coli maintained under carbon starvation stress. Mutants of increased fitness express a growth advantage in stationary phase (GASP) phenotype, enabling them to grow and displace the parent as the majority population. The first GASP mutation was identified as a loss-of-function allele of rpoS, encoding the stationary-phase global regulator, sigma(S) (M. M. Zambrano, D. A. Siegele, M. A. Almirón, A. Tormo, and R. Kolter, Science 259:1757-1760, 1993). We now report that a second global regulator, Lrp, can also play a role in stationary-phase competition. We found that a mutant that took over an aged culture of an rpoS strain had acquired a GASP mutation in lrp. This GASP allele, lrp-1141, encodes a mutant protein lacking the critical glycine in the turn of the helix-turn-helix DNA-binding domain. The lrp-1141 allele behaves as a null mutation when in single copy and is dominant negative when overexpressed. Hence, the mutant protein appears to retain stability and the ability to dimerize but lacks DNA-binding activity. We also demonstrated that a lrp null allele generated by a transposon insertion has a fitness gain identical to that of the lrp-1141 allele, verifying that cells lacking Lrp activity have a competitive advantage during prolonged starvation. Finally, we tested by genetic analysis the hypothesis that the lrp-1141 GASP mutation confers a fitness gain by enhancing amino acid catabolism during carbon starvation. We found that while amino acid catabolism may play a role, it is not necessary for the lrp GASP phenotype, and hence the lrp GASP phenotype is due to more global physiological changes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zinser ER,Kolter Rdoi
10.1128/jb.182.15.4361-4365.2000keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
4361-5issue
15eissn
0021-9193issn
1098-5530journal_volume
182pub_type
杂志文章abstract：:Enteropathogenic Escherichia coli employs a type III secretion system (T3SS) to translocate virulence effector proteins directly into enterocyte host cells, leading to diarrheal disease. The T3SS is encoded within the chromosomal locus of enterocyte effacement (LEE). The function of some of the LEE-encoded proteins re...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01215-12
更新日期：2012-11-01 00:00:00
abstract：:Membrane vesicles capable of energy-dependent calcium uptake have been prepared from Bacillus megaterium cells in log-phase growth or when undergoing sporulation. The uptake is dependent on the calcium concentration and appears saturable in vesicles from cells in log-phase growth. Both ascorbate and phenazine methosul...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.119.3.840-843.1974
更新日期：1974-09-01 00:00:00
abstract：:Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.181.7.2209-2216.1999
更新日期：1999-04-01 00:00:00
abstract：:The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show th...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.01472-10
更新日期：2011-04-01 00:00:00
abstract：:Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.170.3.1245-1253.1988
更新日期：1988-03-01 00:00:00
abstract：:Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.138.2.638-641.1979
更新日期：1979-05-01 00:00:00
abstract：:Analysis of the deoxyribonucleic acid prepared from Proteus mirabilis, Escherichia coli, and Serratia marcescens in an alkaline CsCl gradient has shown that there is an asymmetric distribution of guanine plus thymine residues between the complementary strands of the deoxyribonucleic acid. ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.106.1.238-242.1971
更新日期：1971-04-01 00:00:00
abstract：:Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.90.6.1611-1616.1965
更新日期：1965-12-01 00:00:00
abstract：:Numerous bacteria and mammalian cells harbor two enzymes, phosphopentomutase (PPM) and 2-deoxyribose 5-phosphate aldolase (DERA), involved in the interconversion between nucleosides and central carbon metabolism. In this study, we have examined the presence of this metabolic link in the hyperthermophilic archaeon, The...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.186.13.4185-4191.2004
更新日期：2004-07-01 00:00:00
abstract：:Anabaenopeptins (AP) are bioactive cyclic hexapeptides synthesized nonribosomally in cyanobacteria. APs are characterized by several conserved motifs, including the ureido bond, N-methylation in position 5, and d-Lys in position 2. All other positions of the AP molecule are variable, resulting in numerous structural v...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00360-11
更新日期：2011-08-01 00:00:00
abstract：:In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.164.2.854-860.1985
更新日期：1985-11-01 00:00:00
abstract：:FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00074-17
更新日期：2017-04-25 00:00:00
abstract：:All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00630-13
更新日期：2013-10-01 00:00:00
abstract：:Domingue, Gerald J. (Tulane University, New Orleans, La.), and William A. Pierce, Jr. Effect of partially purified streptococcal M protein on the in vitro phagocytosis of Streptococcus pyogenes. J. Bacteriol. 89:583-588. 1965.-M protein from Streptococcus pyogenes strain Bailey type 12 was adsorbed onto cells of a glo...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.89.3.583-588.1965
更新日期：1965-03-01 00:00:00
abstract：:Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.120.1.565-567.1974
更新日期：1974-10-01 00:00:00
abstract：:Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.00150-20
更新日期：2020-08-25 00:00:00
abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Immunogenic activity of a ribosomal fraction obtained from Mycobacterium tuberculosis. J. Bacteriol. 89:1291-1298. 1965.-The highly immunogenic particulate fraction obtained from mechanically ruptured cells of the H37Ra strai...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.89.5.1291-1298.1965
更新日期：1965-05-01 00:00:00
abstract：:The Bacillus subtilis sbo-alb operon contains sboA, the structural gene for the bacteriocin subtilosin, and the alb genes required for subtilosin production. Transcription from the sbo-alb promoter is highly induced by oxygen limitation. The transcriptional regulation of the sbo-alb operon is under dual control involv...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.182.11.3274-3277.2000
更新日期：2000-06-01 00:00:00
abstract：:The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a reg...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.97.3.1118-1128.1969
更新日期：1969-03-01 00:00:00
abstract：:Shigella flexneri is a gram-negative bacterium that causes diarrhea and dysentery by invasion and spread through the colonic epithelium. Bacteria spread by assembling actin and other cytoskeletal proteins of the host into "actin tails" at the bacterial pole; actin tail assembly provides the force required to move bact...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.181.3.869-878.1999
更新日期：1999-02-01 00:00:00
abstract：:The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.178.19.5797-5802.1996
更新日期：1996-10-01 00:00:00
abstract：:In order to clarify the relationship between polyploidization and the capability of phenotypic switching in the imperfect yeast Candida albicans, two types of variants were isolated as segregants from a fusant, which produced a proportion of the cell population with a higher ploidy than the rest, either in a temperatu...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.176.11.3345-3353.1994
更新日期：1994-06-01 00:00:00
abstract：:Henle, Gertrude (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Werner Henle. Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bacteriol. 91:1248-1256. 1966.-Indirect immunofluorescence tests led to the brilliant staining of a small proportion of the cells in five different cultures derive...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.91.3.1248-1256.1966
更新日期：1966-03-01 00:00:00
abstract：:Antibiotic resistance carried on R factors was transferred by conjugation from Escherichia coli B/r and Shigella flexneri 1a to Erwinia spp. Tetracycline resistance (TetR) carried on R factor R100 drd-56 was transferred from E. coli B/r to strains of Erwinia amylovora, E. aroideae, E. atroseptica, E. chrysanthemi, E. ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.112.1.576-584.1972
更新日期：1972-10-01 00:00:00
abstract：:Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.100.2.687-694.1969
更新日期：1969-11-01 00:00:00
abstract：:Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.179.2.310-316.1997
更新日期：1997-01-01 00:00:00
abstract：:The glycosyltransferase (GT) module of class A penicillin-binding proteins (PBPs) and monofunctional GTs (MGTs) belong to the GT51 family in the sequence-based classification of GTs. They both possess five conserved motifs and use lipid II precursor (undecaprenyl-pyrophosphate-N-acetylglucosaminyl-N-acetylmuramoyl- pe...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.188.7.2528-2532.2006
更新日期：2006-04-01 00:00:00
abstract：:When an Escherichia coli K-12 culture was starved for glucose, 50% of the cells lost viability in about 6 days. When a K-12 mutant lacking five distinct peptidase activities, CM89, was starved in the same manner, viability was lost much more rapidly; 50% of the cells lost viability in about 2 days, whereas a parent st...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.157.3.758-763.1984
更新日期：1984-03-01 00:00:00
abstract：:Candida albicans produces large amounts of the acyclic pentitol D-arabitol in culture and in infected animals and humans, and most strains also grow on minimal D-arabitol medium. An earlier study showed that the major metabolic precursor of D-arabitol in C. albicans was D-ribulose-5-PO4 from the pentose pathway, that ...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/jb.177.11.2971-2976.1995
更新日期：1995-06-01 00:00:00
abstract：:In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...
journal_title：Journal of bacteriology
pub_type： 杂志文章
doi：10.1128/JB.135.3.1062-1069.1978
更新日期：1978-09-01 00:00:00
