EFFECT OF PARTIALLY PURIFIED STREPTOCOCCAL M PROTEIN ON THE IN VITRO PHAGOCYTOSIS OF STREPTOCOCCUS PYOGENES.

Abstract:

:Domingue, Gerald J. (Tulane University, New Orleans, La.), and William A. Pierce, Jr. Effect of partially purified streptococcal M protein on the in vitro phagocytosis of Streptococcus pyogenes. J. Bacteriol. 89:583-588. 1965.-M protein from Streptococcus pyogenes strain Bailey type 12 was adsorbed onto cells of a glossy mutant of this strain. Phagocytosis experiments were performed in vitro with rabbit peritoneal polymorphonuclear leukocytes. Direct microscopic counts suggested M protein-treated cells were more resistant to phagocytosis than were untreated cells. With the Cohn and Morse technique for evaluating quantitatively the fate of bacteria during phagocytosis, there was observed a more rapid drop in total and extracellular counts of treated, as compared with untreated, cells. Experiments with radioactively labeled streptococci showed that M-treated cells were more readily destroyed within leukocytes than were nontreated glossy streptococci, which suggested a reason for the scarcity of treated streptococci in direct microscopic counts.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

DOMINGUE GJ,PIERCE WA Jr

doi

10.1128/JB.89.3.583-588.1965

keywords:

subject

Has Abstract

pub_date

1965-03-01 00:00:00

pages

583-8

eissn

0021-9193

issn

1098-5530

journal_volume

89

pub_type

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