Chemotaxis in Borrelia burgdorferi.

Abstract:

:Borrelia burgdorferi is a motile spirochete which has been identified as the causative microorganism in Lyme disease. The physiological functions which govern the motility of this organism have not been elucidated. In this study, we found that motility of B. burgdorferi required an environment similar to interstitial fluid (e.g., pH 7.6 and 0.15 M NaCl). Several methods were used to detect and measure chemotaxis of B. burgdorferi. A number of chemical compounds and mixtures were surveyed for the ability to induce positive and negative chemotaxis of B. burgdorferi. Rabbit serum was found to be an attractant for B. burgdorferi, while ethanol and butanol were found to be repellents. Unlike some free-living spirochetes (e.g., Spirochaeta aurantia), B. burgdorferi did not exhibit any observable chemotaxis to common sugars or amino acids. A method was developed to produce spirochete cells with a self-entangled end. These cells enabled us to study the rotation of a single flagellar bundle in response to chemoattractants or repellents. The study shows that the frequency and duration for pausing of flagella are important for chemotaxis of B. burgdorferi.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Shi W,Yang Z,Geng Y,Wolinsky LE,Lovett MA

doi

10.1128/JB.180.2.231-235.1998

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

231-5

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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