Burkholderia spp. alter Pseudomonas aeruginosa physiology through iron sequestration.

Abstract:

:Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced in the presence of Burkholderia: A random gene fusion library was constructed in P. aeruginosa PA14 by using a transposon containing a promoterless lacZ gene. Fusion strains were screened for their ability to be induced in the presence of Burkholderia strains in a cross-streak assay. Three fusion strains were induced specifically by Burkholderia species; all three had transposon insertions in genes known to be iron regulated. One of these fusion strains, containing a transposon insertion in gene PA4467, was used to characterize the inducing activity from Burkholderia: Biochemical and genetic evidence demonstrate that ornibactin, a siderophore produced by nearly all B. cepacia strains, can induce P. aeruginosa PA4467. Significantly, PA4467 is induced early in coculture with an ornibactin-producing but not an ornibactin-deficient B. cepacia strain, indicating that ornibactin can be produced by B. cepacia and detected by P. aeruginosa when the two species coexist.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Weaver VB,Kolter R

doi

10.1128/jb.186.8.2376-2384.2004

keywords:

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

2376-84

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

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