TPW22, a lactococcal temperate phage with a site-specific integrase closely related to Streptococcus thermophilus phage integrases.

Abstract:

:The temperate phage TPW22, induced from Lactococcus lactis subsp. cremoris W22, and the evolutionarily interesting integrase of this phage were characterized. Phage TPW22 was propagated lytically on L. lactis subsp. cremoris 3107, which could also be lysogenized by site-specific integration. The attachment site (attP), 5'-TAAGGCGACGGTCG-3', of phage TPW22 was present on a 7.5-kb EcoRI fragment, a 3.4-kb EcoRI-HindIII fragment of which was sequenced. Sequence information revealed the presence of an integrase gene (int). The deduced amino acid sequence showed 42 and 28% identity with integrases of streptococcal and lactococcal phages, respectively. The identities with these integrase-encoding genes were 52 and 45%, respectively, at the nucleotide level. This could indicate horizontal gene transfer. A stable integration vector containing attP and int was constructed, and integration in L. lactis subsp. cremoris MG1363 was obtained. The existence of an exchangeable lactococcal phage integration module was suggested. The proposed module covers the phage attachment site, the integrase gene, and surrounding factor-independent terminator structures. The phages phiLC3, TP901-1, and TPW22 all have different versions of this module. Phylogenetically, the TPW22 Int links the phiLC3 lactococcal integrase with known Streptococcus thermophilus integrases.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Petersen A,Josephsen J,Johnsen MG

doi

10.1128/JB.181.22.7034-7042.1999

keywords:

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

7034-42

issue

22

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

杂志文章
  • Mobilization of Haemophilus influenzae chromosomal markers by an Escherichia coli F' factor.

    abstract::Filter matings between E. coli K-12 strains carrying an F'::Tn5,Tn9 factor with H. influenzae Rd strains gave rise to kanamycin-chloramphenicol-resistant H. influenzae strains at a frequency of approximately 10(-6). Transfer of the F' factor to H. influenzae was verified by expression of unselected markers in H. influ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.1905-1910.1987

    authors: Deich RA,Green BA

    更新日期:1987-05-01 00:00:00

  • Role of secondary transporters and phosphotransferase systems in glucose transport by Oenococcus oeni.

    abstract::Glucose uptake by the heterofermentative lactic acid bacterium Oenococcus oeni B1 was studied at the physiological and gene expression levels. Glucose- or fructose-grown bacteria catalyzed uptake of [(14)C]glucose over a pH range from pH 4 to 9, with maxima at pHs 5.5 and 7. Uptake occurred in two-step kinetics in a h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06038-11

    authors: Kim OB,Richter H,Zaunmüller T,Graf S,Unden G

    更新日期:2011-12-01 00:00:00

  • Cell wall turnover in batch and chemostat cultures of Bacillus subtilis.

    abstract::Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.50-60.1981

    authors: De Boer WR,Kruyssen FJ,Wouters JT

    更新日期:1981-01-01 00:00:00

  • The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration.

    abstract::Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.16.4721-4729.1995

    authors: Popham DL,Illades-Aguiar B,Setlow P

    更新日期:1995-08-01 00:00:00

  • Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase.

    abstract::At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at the late stages of sporulation. We show here that the longer transcript contains ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.4885-4892.1992

    authors: Wu JJ,Schuch R,Piggot PJ

    更新日期:1992-08-01 00:00:00

  • A New ESX-1 Substrate in Mycobacterium marinum That Is Required for Hemolysis but Not Host Cell Lysis.

    abstract::The ESX-1 (ESAT-6 system 1) secretion system plays a conserved role in the virulence of diverse mycobacterial pathogens, including the human pathogen Mycobacterium tuberculosis and M. marinum, an environmental mycobacterial species. The ESX-1 system promotes the secretion of protein virulence factors to the extracytop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00760-18

    authors: Bosserman RE,Nicholson KR,Champion MM,Champion PA

    更新日期:2019-06-21 00:00:00

  • The pneumococcal cell envelope stress-sensing system LiaFSR is activated by murein hydrolases and lipid II-interacting antibiotics.

    abstract::In the Firmicutes, two-component regulatory systems of the LiaSR type sense and orchestrate the response to various agents that perturb cell envelope functions, in particular lipid II cycle inhibitors. In the current study, we found that the corresponding system in Streptococcus pneumoniae displays similar properties ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01489-09

    authors: Eldholm V,Gutt B,Johnsborg O,Brückner R,Maurer P,Hakenbeck R,Mascher T,Håvarstein LS

    更新日期:2010-04-01 00:00:00

  • Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae.

    abstract::XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to clon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.14.4183-4186.1995

    authors: Sirois S,Szatmari G

    更新日期:1995-07-01 00:00:00

  • Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1.

    abstract::Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1707-1711.1996

    authors: Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

    更新日期:1996-03-01 00:00:00

  • DNA microarray analysis of genome dynamics in Yersinia pestis: insights into bacterial genome microevolution and niche adaptation.

    abstract::Genomics research provides an unprecedented opportunity for us to probe into the pathogenicity and evolution of the world's most deadly pathogenic bacterium, Yersinia pestis, in minute detail. In our present work, extensive microarray analysis in conjunction with PCR validation revealed that there are considerable gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.15.5138-5146.2004

    authors: Zhou D,Han Y,Song Y,Tong Z,Wang J,Guo Z,Pei D,Pang X,Zhai J,Li M,Cui B,Qi Z,Jin L,Dai R,Du Z,Bao J,Zhang X,Yu J,Wang J,Huang P,Yang R

    更新日期:2004-08-01 00:00:00

  • Release of the -galactosidase-synthesizing system from ultraviolet catabolite repression by cyclic 3',5'-adenosine monophosphate, dark repair, photoreactivation, and cold treatment.

    abstract::Recovery from the inhibitory effect of ultraviolet irradiation on the induced synthesis of beta-galactosidase was studied in Escherichia coli B/r. When irradiated cells (520 ergs/mm(2) at 254 nm) were induced and incubated in minimal medium supplemented with Casamino Acids (conditions of catabolite repression), the ab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.391-398.1972

    authors: Swenson PA

    更新日期:1972-01-01 00:00:00

  • Cloning and partial sequencing of an operon encoding two Pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity.

    abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.8.2612-2619.1992

    authors: Barth PT,Bolton L,Thomson JC

    更新日期:1992-04-01 00:00:00

  • Distribution of megaplasmids in Lactobacillus salivarius and other lactobacilli.

    abstract::The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-07

    authors: Li Y,Canchaya C,Fang F,Raftis E,Ryan KA,van Pijkeren JP,van Sinderen D,O'Toole PW

    更新日期:2007-09-01 00:00:00

  • Factors affecting cellulolysis by Ruminococcus albus.

    abstract::The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.729-737.1973

    authors: Smith WR,Yu I,Hungate RE

    更新日期:1973-05-01 00:00:00

  • Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein.

    abstract::The Vibrio fischeri luminescence (lux) genes are regulated by the 250-amino-acid-residue LuxR protein and a V. fischeri metabolite termed autoinducer. The V. fischeri lux regulon consists of two divergently transcribed units. Autoinducer and LuxR activate transcription of the luxICDABE operon and autoregulate the luxR...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.12.4064-4069.1992

    authors: Choi SH,Greenberg EP

    更新日期:1992-06-01 00:00:00

  • Stability of the Escherichia coli division inhibitor protein MinC requires determinants in the carboxy-terminal region of the protein.

    abstract::Certain mutations in the C-terminal region of the Escherichia coli division inhibitor protein MinC cause loss of function of the division inhibitor by making MinC more sensitive to degradation by Lon protease, implying a possible role for the C-terminal region in regulating the stability and cellular concentration of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.1.175-177.1998

    authors: Sen M,Rothfield LI

    更新日期:1998-01-01 00:00:00

  • Bacteriostatic activity of serum against staphylococci.

    abstract::Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.953-962.1966

    authors: Cybulska J,Jeljaszewicz J

    更新日期:1966-03-01 00:00:00

  • Fine structure of Vibrio cholerae during toxin production.

    abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1393-1401.1969

    authors: Kennedy JR,Richardson SH

    更新日期:1969-12-01 00:00:00

  • Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

    abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.468-470.1988

    authors: Berg CM,Liu L,Wang B,Wang MD

    更新日期:1988-01-01 00:00:00

  • Metabolism of phospholipid 2-linked fatty acids during the release of membrane fragments from Haemophilus parainfluenzae by ethylenediaminetetraacetic acid-tris(hydroxymethyl)-aminomethane.

    abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.2.329-334.1970

    authors: Tucker AN,White DC

    更新日期:1970-08-01 00:00:00

  • Genetic mapping of the chromosomal replication origin of Salmonella typhimurium.

    abstract::Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.2.297-304.1979

    authors: Joh K,Hiraga S

    更新日期:1979-05-01 00:00:00

  • Properties of 2-oxoglutarate:ferredoxin oxidoreductase from Thauera aromatica and its role in enzymatic reduction of the aromatic ring.

    abstract::Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.14.3975-3983.2002

    authors: Dörner E,Boll M

    更新日期:2002-07-01 00:00:00

  • Antibody response to Staphylococcus aureus in rabbits: sequence of immunoglobulin synthesis and its correlation with passive protection in mice.

    abstract::Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in nor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1540-1545.1968

    authors: Yoshida K,Ekstedt RD

    更新日期:1968-11-01 00:00:00

  • Partial functional replacement of CymA by SirCD in Shewanella oneidensis MR-1.

    abstract::The gammaproteobacterium Shewanella oneidensis MR-1 utilizes a complex electron transfer network composed primarily of c-type cytochromes to respire under anoxic conditions a variety of compounds, including fumarate, nitrate, and dimethyl sulfoxide (DMSO), in addition to the minerals Fe(III) and Mn(IV). Central to sev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01355-10

    authors: Cordova CD,Schicklberger MF,Yu Y,Spormann AM

    更新日期:2011-05-01 00:00:00

  • Archaeal intrinsic transcription termination in vivo.

    abstract::Thermococcus kodakarensis (formerly Thermococcus kodakaraensis) strains have been constructed with synthetic and natural DNA sequences, predicted to function as archaeal transcription terminators, identically positioned between a constitutive promoter and a beta-glycosidase-encoding reporter gene (TK1761). Expression ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00982-09

    authors: Santangelo TJ,Cubonová L,Skinner KM,Reeve JN

    更新日期:2009-11-01 00:00:00

  • The linker region of AraC protein.

    abstract::AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Her...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.24.7025-7030.1996

    authors: Eustance RJ,Schleif RF

    更新日期:1996-12-01 00:00:00

  • Partial characterization of lipid A of intraperiplasmically grown Bdellovibrio bacteriovorus.

    abstract::The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.3.869-874.1981

    authors: Nelson DR,Rittenberg SC

    更新日期:1981-09-01 00:00:00

  • Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T.

    abstract::Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00614-08

    authors: Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

    更新日期:2008-08-01 00:00:00

  • Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.

    abstract::At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.658-667.1970

    authors: Dempsey WB,Ito H

    更新日期:1970-11-01 00:00:00

  • Fluorescence High-Throughput Screening for Inhibitors of TonB Action.

    abstract::Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00889-16

    authors: Nairn BL,Eliasson OS,Hyder DR,Long NJ,Majumdar A,Chakravorty S,McDonald P,Roy A,Newton SM,Klebba PE

    更新日期:2017-04-25 00:00:00