Abstract:
:Spores of Bacillus subtilis have a thick outer layer of relatively insoluble protein called the coat, which protects spores against a number of treatments and may also play roles in spore germination. However, elucidation of precise roles of the coat in spore properties has been hampered by the inability to prepare spores lacking all or most coat material. In this work, we show that spores of a strain with mutations in both the cotE and gerE genes, which encode proteins involved in coat assembly and expression of genes encoding coat proteins, respectively, lack most extractable coat protein as seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as well as the great majority of the coat as seen by atomic force microscopy. However, the cotE gerE spores did retain a thin layer of insoluble coat material that was most easily seen by microscopy following digestion of these spores with lysozyme. These severely coat-deficient spores germinated relatively normally with nutrients and even better with dodecylamine but not with a 1:1 chelate of Ca(2+) and dipicolinic acid. These spores were also quite resistant to wet heat, to mechanical disruption, and to treatment with detergents at an elevated temperature and pH but were exquisitely sensitive to killing by sodium hypochlorite. These results provide new insight into the role of the coat layer in spore properties.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ghosh S,Setlow B,Wahome PG,Cowan AE,Plomp M,Malkin AJ,Setlow Pdoi
10.1128/JB.00896-08subject
Has Abstractpub_date
2008-10-01 00:00:00pages
6741-8issue
20eissn
0021-9193issn
1098-5530pii
JB.00896-08journal_volume
190pub_type
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1978-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05982-11
更新日期:2012-04-01 00:00:00
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更新日期:1982-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1984-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
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abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
abstract::The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5637-5642.1990
更新日期:1990-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00642-08
更新日期:2008-10-01 00:00:00
abstract::Rogosa, M. (National Institutes of Health, Bethesda, Md.). The genus Veillonella. I. General cultural, ecological, and biochemical considerations. J. Bacteriol. 87:162-170. 1964.-Arguments are presented for excluding Veillonella discoides, V. reniformis, V. orbiculus, and V. vulvovaginitidis from the genus, and for re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.1.162-170.1964
更新日期:1964-01-01 00:00:00