A mutant of Mycobacterium smegmatis defective in dipeptide transport.

abstract：:From the marine sponge Halichondria panicea a lectin was isolated and characterized. The homogeneous lectin (composed of protein to 80.7% and of neutral carbohydrates to 14.1%) had a molecular weight of 78,000 (determined by gel filtration) and consisted of four subunits with a molecular weight of 21,000 each (determi...

journal_title：Journal of bacteriology

authors： Müller WE,Zahn RK,Kurelec B,Lucu C,Müller I,Uhlenbruck G

更新日期：1981-01-01 00:00:00

abstract：:Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

journal_title：Journal of bacteriology

authors： van Solingen P,van der Plaat JB

更新日期：1977-05-01 00:00:00

abstract：:A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...

journal_title：Journal of bacteriology

authors： Poulter R,Hanrahan V

更新日期：1983-11-01 00:00:00

abstract：:Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

journal_title：Journal of bacteriology

authors： Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

更新日期：2002-04-01 00:00:00

abstract：:The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

journal_title：Journal of bacteriology

authors： van Keulen G,Dijkhuizen L,Meijer WG

更新日期：2000-08-01 00:00:00

abstract：:Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

journal_title：Journal of bacteriology

authors： Tisa LS,Ensign JC

更新日期：1988-07-01 00:00:00

abstract：:Fitz-James, Philip (University of Western Ontario, London, Canada). Electron microscopy of Bacillus megaterium undergoing isolation of its nucelar bodies. J. Bacteriol. 87:1202-1210. 1964.-The various steps of treatment leading to the isolation of nuclear bodies were followed by thin-section electron microscopy. Nucle...

journal_title：Journal of bacteriology

authors： Fitz-James P

更新日期：1964-05-01 00:00:00

abstract：:A second synchrony induction treatment, half-way through the deoxyribonucleic acid replication process of an already synchronized culture, resulted in defective subsequent cell growth. This supports a previous explanation for the partial loss of viability after synchrony induction. ...

journal_title：Journal of bacteriology

authors： Herdman M,Carr NG

更新日期：1971-08-01 00:00:00

abstract：:Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of beta-glucosidase activity increased significantly during the emergence stage of germination. Both alpha-glucosidase and N-ace...

journal_title：Journal of bacteriology

authors： Tisa LS,Cotter DA

更新日期：1980-02-01 00:00:00

abstract：:The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...

journal_title：Journal of bacteriology

authors： Kovacevic S,Miller JR

更新日期：1991-01-01 00:00:00

abstract：:We cloned and sequenced a fragment of the Bacillus stearothermophilus NUB36 chromosome that contains two open reading frames (ORFs) whose products were detected only in cells of cultures grown in complex medium at high temperature. The nucleotide sequence of the two ORFs exhibited significant identity to the sequence ...

journal_title：Journal of bacteriology

authors： Wu L,Welker NE

更新日期：1991-08-01 00:00:00

abstract：:The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...

journal_title：Journal of bacteriology

authors： Buckley ND,Lee LN,LeBlanc DJ

更新日期：1995-09-01 00:00:00

abstract：:Previous microarray studies have suggested that an indirect mechanism of Fur regulation may be present in meningococcus at the posttranscriptional level through a small regulatory RNA (sRNA) system analogous to that of Escherichia coli and Pseudomonas aeruginosa. Recently, a Fur-regulated sRNA, NrrF, was identified th...

journal_title：Journal of bacteriology

authors： Metruccio MM,Fantappiè L,Serruto D,Muzzi A,Roncarati D,Donati C,Scarlato V,Delany I

更新日期：2009-02-01 00:00:00

abstract：:A variant of the diaminopimelate (DAP)-lysine biosynthesis pathway uses an LL-DAP aminotransferase (DapL, EC 2.6.1.83) to catalyze the direct conversion of L-2,3,4,5-tetrahydrodipicolinate to LL-DAP. Comparative genomic analysis and experimental verification of DapL candidates revealed the existence of two diverged fo...

journal_title：Journal of bacteriology

authors： Hudson AO,Gilvarg C,Leustek T

更新日期：2008-05-01 00:00:00

abstract：:Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

journal_title：Journal of bacteriology

authors： FUKUYAMA TT,O'KANE DJ

更新日期：1962-10-01 00:00:00

abstract：:Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...

journal_title：Journal of bacteriology

authors： Beller HR,Spormann AM

更新日期：1998-10-01 00:00:00

abstract：:A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...

journal_title：Journal of bacteriology

authors： Bradshaw HD Jr,Traxler BA,Minkley EG Jr,Nester EW,Gordon MP

更新日期：1990-07-01 00:00:00

abstract：:Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

journal_title：Journal of bacteriology

authors： Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

更新日期：1998-05-01 00:00:00

abstract：:In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...

journal_title：Journal of bacteriology

authors： Eddy CK,Keshav KF,An H,Utt EA,Mejia JP,Ingram LO

更新日期：1991-01-01 00:00:00

abstract：:We present experimental evidence for the existence of multiple activator-binding sites in the upstream sequence of the ompC promoter, the expression of which is activated by the positive regulator OmpR in response to the osmolarity of the medium. We also found that a single OmpR-binding site can activate the ompC prom...

journal_title：Journal of bacteriology

authors： Maeda S,Mizuno T

更新日期：1990-01-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:Myxococcus xanthus RB5, a rough-colony-forming, nondispersed growing mutant of strain FBt, forms macroscopic, multicellular masses of radially oriented cells in shake cultures. The cells appear to be held together by slime fibrils. Physical and enzymatic methods to disrupt the spheres were unsuccessful as were attempt...

journal_title：Journal of bacteriology

authors： Burchard RP

更新日期：1975-04-01 00:00:00

abstract：:The position of the termination codon in lacZX90 was determined by isolation of a lac+ revertant. Lysine was found to replace tyrosine at position 1,012 of beta-galactosidase, indicating that X90 protein lacked the carboxyl-terminal 10 residues. A heat- and urea-sensitive hybrid enzyme was formed in vivo when supC, wh...

journal_title：Journal of bacteriology

authors： Mandecki W,Fowler AV,Zabin I

更新日期：1981-08-01 00:00:00

abstract：:DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

journal_title：Journal of bacteriology

authors： Asai T,Kogoma T

更新日期：1994-11-01 00:00:00

abstract：:The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

journal_title：Journal of bacteriology

authors： Smith WR,Yu I,Hungate RE

更新日期：1973-05-01 00:00:00

abstract：:Glutamine phosphoribosylpyrophosphate amidotransferase is stable in growing cells, but is inactivated in an oxygen-dependent process at various rates in starving or antibiotic-treated cells. On the basis of studies of the purified enzyme, we suggested (D.A. Bernlohr and R.L. Switzer, Biochemistry 20:5675-5681, 1981) t...

journal_title：Journal of bacteriology

authors： Bernlohr DA,Switzer RL

更新日期：1983-02-01 00:00:00

abstract：:During bacteriophage T7 infection, the Escherichia coli RNA polymerase beta' subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr(1068), in the evolutionarily hypervariable segment of ...

journal_title：Journal of bacteriology

authors： Severinova E,Severinov K

更新日期：2006-05-01 00:00:00

abstract：:This paper describes the regulation of a transfer ribonucleic acid (tRNA) biosynthetic enzyme, the tRNA(m5U)methyltransferase (EC 2.1.1.35). This enzyme catalyzes the formation of 5-methyluridine (m5U, ribothymidine) in all tRNA chains of Escherichia coli. Partial deprivation of charged tRNAVal can be imposed by shift...

journal_title：Journal of bacteriology

authors： Ny T,Björk GR

更新日期：1977-05-01 00:00:00

abstract：:The nucleotide sequence of the Escherichia coli colicin I receptor gene (cir) has been determined. The predicted mature protein consists of 599 amino acids and has a molecular weight of 67,169. Several previously noted characteristics of other E. coli outer membrane protein sequences were also identified in the sequen...

journal_title：Journal of bacteriology

authors： Nau CD,Konisky J

更新日期：1989-02-01 00:00:00

abstract：:Lockart, Royce Z., Jr. (The University of Texas, Austin). Variations in the abilities of several lines of L cells to produce and to be affected by interferon. J. Bacteriol. 89:117-122. 1965.-The amounts of interferon produced by three different lines of L cells and mouse embryonic cells when inoculated with two strain...

journal_title：Journal of bacteriology

authors： LOCKART RZ Jr

更新日期：1965-01-01 00:00:00