Position of the lacZX90 mutation and hybridization between complete and incomplete beta-galactosidase.

Abstract:

:The position of the termination codon in lacZX90 was determined by isolation of a lac+ revertant. Lysine was found to replace tyrosine at position 1,012 of beta-galactosidase, indicating that X90 protein lacked the carboxyl-terminal 10 residues. A heat- and urea-sensitive hybrid enzyme was formed in vivo when supC, which supplies tyrosine to the position in the polypeptide corresponding to the nonsense codon, was used to suppress lacZX90. This result shows that suppression that adds back the original amino acid may not lead to the production of the wild-type enzyme if the latter is multimeric, because incomplete chains can be incorporated into the oligomer.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mandecki W,Fowler AV,Zabin I

doi

10.1128/JB.147.2.694-697.1981

subject

Has Abstract

pub_date

1981-08-01 00:00:00

pages

694-7

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

147

pub_type

杂志文章
  • 2-deoxyribose gene-enzyme complex in Salmonella typhimurium. I. Isolation and enzymatic characterization of 2-deoxyribose-negative mutants.

    abstract::Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.2.449-457.1968

    authors: Hoffee PA

    更新日期:1968-02-01 00:00:00

  • Purification and properties of mutant and wild-type diaphorases from Diplococcus pneumoniae.

    abstract::Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1029-1036.1968

    authors: Kreger AS,Olsen RH

    更新日期:1968-10-01 00:00:00

  • Characterization of Brucella suis clpB and clpAB mutants and participation of the genes in stress responses.

    abstract::Pathogens often encounter stressful conditions inside their hosts. In the attempt to characterize the stress response in Brucella suis, a gene highly homologous to Escherichia coli clpB was isolated from Brucella suis, and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress resp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2677-2681.2001

    authors: Ekaza E,Teyssier J,Ouahrani-Bettache S,Liautard JP,Köhler S

    更新日期:2001-04-01 00:00:00

  • Nucleotide sequence and regulation of a gene involved in bile acid 7-dehydroxylation by Eubacterium sp. strain VPI 12708.

    abstract::Eubacterium sp. strain VPI 12708 is an anaerobic intestinal bacterium that has inducible bile acid 7-dehydroxylation activity. At least four new polypeptides were synthesized after addition of primary bile acids to the growth medium. One of these, of molecular weight 27,000 (P-27), was shown to be involved in the 7-de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.5.2070-2077.1988

    authors: Coleman JP,White WB,Lijewski M,Hylemon PB

    更新日期:1988-05-01 00:00:00

  • Mechanism of chloride elimination from 3-chloro- and 2,4-dichloro-cis,cis-muconate: new insight obtained from analysis of muconate cycloisomerase variant CatB-K169A.

    abstract::Chloromuconate cycloisomerases of bacteria utilizing chloroaromatic compounds are known to convert 3-chloro-cis,cis-muconate to cis-dienelactone (cis-4-carboxymethylenebut-2-en-4-olide), while usual muconate cycloisomerases transform the same substrate to the bacteriotoxic protoanemonin. Formation of protoanemonin req...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.15.4551-4561.2001

    authors: Kaulmann U,Kaschabek SR,Schlömann M

    更新日期:2001-08-01 00:00:00

  • Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.

    abstract::The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by independent translation initiation at two start codons within the trfA open reading frame. These proteins were o overproduced in Escherichia col...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3849-3858.1990

    authors: Durland RH,Helinski DR

    更新日期:1990-07-01 00:00:00

  • A Rhodobacter capsulatus member of a universal permease family imports molybdate and other oxyanions.

    abstract::Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00742-10

    authors: Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

    更新日期:2010-11-01 00:00:00

  • In vivo interaction of endotoxin with a plasma lipoprotein having esterase activity.

    abstract::Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2031-2034.1968

    authors: Skarnes RC

    更新日期:1968-06-01 00:00:00

  • Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine.

    abstract::Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.6.1306-1312.1962

    authors: SATO I,TANAKA T,SAITO K,MITSUHASHI S

    更新日期:1962-06-01 00:00:00

  • Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.

    abstract::We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.2.433-443.2002

    authors: Prudhomme M,Turlan C,Claverys JP,Chandler M

    更新日期:2002-01-01 00:00:00

  • Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.

    abstract::The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.819-824.1986

    authors: Friedberg F,Rhodes C

    更新日期:1986-03-01 00:00:00

  • Differential inactivation of alcohol dehydrogenase isoenzymes in Zymomonas mobilis by oxygen.

    abstract::Zymomonas mobilis is endowed with two isoenzymes of fermentative alcohol dehydrogenase, a zinc-containing enzyme (ADH I) and an iron-containing enzyme (ADH II). The activity of ADH I remains fully conserved, while ADH II activity decays when anaerobic cultures are shifted to aerobiosis. This differential response depe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.4.1102-1104.1997

    authors: Tamarit J,Cabiscol E,Aguilar J,Ros J

    更新日期:1997-02-01 00:00:00

  • Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli.

    abstract::Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.5.1402-1406.2002

    authors: Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJ

    更新日期:2002-03-01 00:00:00

  • Similarity in the size and number of ribosomal proteins from different prokaryotes.

    abstract::The ribosomal proteins from nine species of prokaryotes have been compared by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The stained gels were scanned spectrophotometrically, the weight and number average molecular weights were calculated, and the detailed distribution of the protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.474-480.1972

    authors: Sun TT,Bickle TA,Traut RR

    更新日期:1972-08-01 00:00:00

  • Regulation of Biofilm Aging and Dispersal in Bacillus subtilis by the Alternative Sigma Factor SigB.

    abstract::Bacterial biofilms are important in natural settings, biotechnology, and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00473-18

    authors: Bartolini M,Cogliati S,Vileta D,Bauman C,Rateni L,Leñini C,Argañaraz F,Francisco M,Villalba JM,Steil L,Völker U,Grau R

    更新日期:2018-12-20 00:00:00

  • Kinetics of uptake and incorporation of meso-diaminopimelic acid in different Escherichia coli strains.

    abstract::The rate at which the peptidoglycan precursor meso-diaminopimelic acid (DAP) is incorporated into the cell wall of Escherichia coli cells was determined by pulse-label experiments. For different E. coli strains, the incorporation rate was compared with the rate of uptake of DAP into the cell. With E. coli W7, a dap ly...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.331-337.1985

    authors: Wientjes FB,Pas E,Taschner PE,Woldringh CL

    更新日期:1985-10-01 00:00:00

  • Insight into the Dual Functions of Bacterial Enhancer-Binding Protein Rrp2 of Borrelia burgdorferi.

    abstract:UNLABELLED:It is well established that the RpoN-RpoS sigma factor (σ(54)-σ(S)) cascade plays an essential role in differential gene expression during the enzootic cycle of Borrelia burgdorferi, the causative agent of Lyme disease. The RpoN-RpoS pathway is activated by the response regulator/σ(54)-dependent activator (a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01010-15

    authors: Yin Y,Yang Y,Xiang X,Wang Q,Yang ZN,Blevins J,Lou Y,Yang XF

    更新日期:2016-04-28 00:00:00

  • The global responses of Mycobacterium tuberculosis to physiological levels of copper.

    abstract::Copper (Cu) is a required micronutrient, but it is highly toxic at high concentrations. Therefore, the levels of Cu must be tightly regulated in all living cells. The phagosome of Mycobacterium tuberculosis has been shown to have variable levels of Cu. Previously, we showed that M. tuberculosis contains a copper-sensi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01847-07

    authors: Ward SK,Hoye EA,Talaat AM

    更新日期:2008-04-01 00:00:00

  • In vitro sulfotransferase activity of NodH, a nodulation protein of Rhizobium meliloti required for host-specific nodulation.

    abstract::Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.21.6237-6245.1995

    authors: Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

    更新日期:1995-11-01 00:00:00

  • Genome sequence and comparative genomics analysis of a Vibrio cholerae O1 strain isolated from a cholera patient in Malaysia.

    abstract::The genome sequence analysis of a clinical Vibrio cholerae VC35 strain from an outbreak case in Malaysia indicates multiple genes involved in host adaptation and a novel Na(+)-driven multidrug efflux pump-coding gene in the genome of Vibrio cholerae with the highest similarity to VMA_001754 of Vibrio mimicus VMA223. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01832-12

    authors: Osama A,Gan HM,Teh CS,Yap KP,Thong KL

    更新日期:2012-12-01 00:00:00

  • Properties of the lysyl-tRNA synthetase gene and product from the extreme thermophile Thermus thermophilus.

    abstract::A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.9.2699-2705.1994

    authors: Chen J,Brevet A,Lapadat-Tapolsky M,Blanquet S,Plateau P

    更新日期:1994-05-01 00:00:00

  • Chromosomal mapping of Bacillus thuringiensis by transduction.

    abstract::Three groups of linked markers were mapped in Bacillus thuringiensis 4042B by using two-, three-, and four-factor crosses mediated by the temperate bacteriophages TP-13 and TP-18. The order of markers was (trp-11, trp-2)-(leu-1, leu-2)-his-1-(lys-1, lys-2)-cys-1 in the first group; met-1-(argCl, argOl)-met-2-(pyr-1, p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.746-750.1984

    authors: Barsomian GD,Robillard NJ,Thorne CB

    更新日期:1984-03-01 00:00:00

  • Bacteriochlorophyll and heme synthesis in Rhodopseudomonas spheroides: possible role of heme in regulation of the branched biosynthetic pathway.

    abstract::Synthesis of heme, measured by incorporation of iron-59, and of bacteriochlorophyll was studied with wild-type and mutant strains of Rhodopseudomonas spheroides. The wild type formed heme from glycine and succinate at one-fortieth the rate of bacteriochlorophyll under anaerobic-light conditions. Added delta-aminolevul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.712-720.1969

    authors: Lascelles J,Hatch TP

    更新日期:1969-05-01 00:00:00

  • asmB, a suppressor locus for assembly-defective OmpF mutants of Escherichia coli, is allelic to envA (lpxC).

    abstract::A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.17.5138-5143.1996

    authors: Kloser AW,Laird MW,Misra R

    更新日期:1996-09-01 00:00:00

  • Organization and transcriptional regulation of the Escherichia coli K-12 D-serine tolerance locus.

    abstract::We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6456-6461.1995

    authors: Nørregaard-Madsen M,McFall E,Valentin-Hansen P

    更新日期:1995-11-01 00:00:00

  • Activation of ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase) in Streptomyces antibioticus.

    abstract::The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1482-1487.1994

    authors: Jones GH

    更新日期:1994-03-01 00:00:00

  • Transcription of Bacillus subtilis degR is sigma D dependent and suppressed by multicopy proB through sigma D.

    abstract::Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.1.216-222.1996

    authors: Ogura M,Tanaka T

    更新日期:1996-01-01 00:00:00

  • Characterization of the extracellular cellulase from a mesophilic clostridium (strain C7).

    abstract::An extracellular, 700,000-Mr multiprotein complex that catalyzed the hydrolysis of crystalline cellulose (Avicel) was isolated from cultures of Clostridium sp. strain C7, a mesophile from freshwater sediment. In addition to cellulose (Avicel, ball-milled filter paper), the multiprotein complex hydrolyzed carboxymethyl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4231-4237.1990

    authors: Cavedon K,Leschine SB,Canale-Parola E

    更新日期:1990-08-01 00:00:00

  • Conservative duplication of spindle poles during meiosis in Saccharomyces cerevisiae.

    abstract::During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.7.2372-2375.2001

    authors: Wesp A,Prinz S,Fink GR

    更新日期:2001-04-01 00:00:00

  • The leucine-responsive regulatory protein, Lrp, activates transcription of the fim operon in Salmonella enterica serovar typhimurium via the fimZ regulatory gene.

    abstract::The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01388-07

    authors: McFarland KA,Lucchini S,Hinton JC,Dorman CJ

    更新日期:2008-01-01 00:00:00