Abstract:
:Zymomonas mobilis is endowed with two isoenzymes of fermentative alcohol dehydrogenase, a zinc-containing enzyme (ADH I) and an iron-containing enzyme (ADH II). The activity of ADH I remains fully conserved, while ADH II activity decays when anaerobic cultures are shifted to aerobiosis. This differential response depends on the metal present on each isoenzyme, since pure preparations of ADH I are resistant to oxidative inactivation and preparations of zinc-containing ADH II, obtained by incubation of pure ADH II with ZnCl2, showed no modification of the target for oxidative damage (His277-containing peptide). It was consistently found that the activity of the zinc-containing ADH II, once submitted to oxidative treatment, was fully restored when iron was reintroduced into the enzyme structure. These results indicate that zinc bound to these proteins plays an important role in the protection of their active centers against oxidative damage and may have relevant biochemical and physiological consequences in this species.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Tamarit J,Cabiscol E,Aguilar J,Ros Jdoi
10.1128/jb.179.4.1102-1104.1997subject
Has Abstractpub_date
1997-02-01 00:00:00pages
1102-4issue
4eissn
0021-9193issn
1098-5530journal_volume
179pub_type
杂志文章abstract::Bacterial lipases play important roles in bacterial metabolism and environmental response. Our laboratory recently discovered that a novel lipoprotein lysophospholipase, VolA, localizes on the surface of the Gram-negative aquatic pathogen Vibrio cholerae. VolA functions to cleave exogenous lysophosphatidylcholine, fre...
journal_title:Journal of bacteriology
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更新日期:2014-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2006-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.92.3.609-617.1966
更新日期:1966-09-01 00:00:00
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更新日期:1992-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.172.12.7275-7277.1990
更新日期:1990-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1380-1383.1971
更新日期:1971-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.1.1-5.1969
更新日期:1969-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.3.1162-1170.1985
更新日期:1985-03-01 00:00:00
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doi:10.1128/JB.93.4.1327-1331.1967
更新日期:1967-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.149-154.1981
更新日期:1981-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.20.6689-6694.1993
更新日期:1993-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.17.5560-5564.1994
更新日期:1994-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.181.16.4978-4985.1999
更新日期:1999-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3558-3560.1992
更新日期:1992-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.1.64-73.1993
更新日期:1993-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.5.750-760.1961
更新日期:1961-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.9.2880-2890.2004
更新日期:2004-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.2.845-857.1980
更新日期:1980-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.24.6913-6920.2000
更新日期:2000-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.5.1979-1986.2006
更新日期:2006-03-01 00:00:00
abstract::A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the sideroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.862-870.1998
更新日期:1998-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.2.710-714.1980
更新日期:1980-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01762-06
更新日期:2007-04-01 00:00:00
abstract::Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3661-3663.1996
更新日期:1996-06-01 00:00:00
abstract::A phosphatase specific for the hydrolysis of 3-deoxy-d-manno-octulosonate (KDO)-8-phosphate was purified approximately 400-fold from crude extracts of Escherichia coli B. The hydrolysis of KDO-8-phosphate to KDO and inorganic phosphate in crude extracts of E. coli B, grown in phosphate-containing minimal medium, could...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.1.60-68.1980
更新日期:1980-04-01 00:00:00