Genetic analysis of plasmid determinants for microcin J25 production and immunity.

Abstract:

:Microcin J25 (MccJ25) is a small peptide antibiotic produced by an Escherichia coli strain isolated from human feces. The genetic determinants for MccJ25 synthesis and immunity have been cloned from the low-copy-number wild-type plasmid pTUC1OO into the compatible vectors pBR322 and pACYC184. Physical and phenotypical analysis of insertion mutations and complementation tests defined three contiguous genes involved in MccJ25 production which span a region of about 2.2 kb. Immunity to the antibiotic is provided by an additional gene adjacent to the production region.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Solbiati JO,Ciaccio M,Farías RN,Salomón RA

doi

10.1128/jb.178.12.3661-3663.1996

subject

Has Abstract

pub_date

1996-06-01 00:00:00

pages

3661-3

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

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