Novel posttranslational activation of the LYS2-encoded alpha-aminoadipate reductase for biosynthesis of lysine and site-directed mutational analysis of conserved amino acid residues in the activation domain of Candida albicans.

abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

journal_title：Journal of bacteriology

authors： Huang AM,Lee JI,King SC,Wilson TH

更新日期：1992-08-01 00:00:00

abstract：:Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...

journal_title：Journal of bacteriology

authors： Gromkova RH

更新日期：1971-06-01 00:00:00

abstract：:We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

journal_title：Journal of bacteriology

authors： Freifelder D,Folkmanis A,Kirschner I

更新日期：1971-03-01 00:00:00

abstract：:We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

journal_title：Journal of bacteriology

authors： Davis LC,Wang YL

更新日期：1980-03-01 00:00:00

abstract：:The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...

journal_title：Journal of bacteriology

authors： Gál J,Szvetnik A,Schnell R,Kálmán M

更新日期：2002-09-01 00:00:00

abstract：:The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which w...

journal_title：Journal of bacteriology

authors： Larkin JM,Stokes JL

更新日期：1967-10-01 00:00:00

abstract：:Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...

journal_title：Journal of bacteriology

authors： Rosenberg B,Renshaw E,Vancamp L,Hartwick J,Drobnik J

更新日期：1967-02-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...

journal_title：Journal of bacteriology

authors： Tawk CS,Ghattas IR,Smith CA

更新日期：2015-11-01 00:00:00

abstract：:The DNA sequence of the central regulatory locus vir of Bordetella pertussis predicts that three gene products, BvgA, BvgB, and BvgC, are encoded. Features of the predicted primary structures of these proteins and their homology to other two-component systems suggest that BvgA is located in the cytoplasm, BvgB is loca...

journal_title：Journal of bacteriology

authors： Stibitz S,Yang MS

更新日期：1991-07-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:The Bacillus subtilis dciA operon encodes a dipeptide transport complex that is induced rapidly as cells enter stationary phase and initiate sporulation. Expression of this operon in growing cells is repressed by glucose, by a mixture of amino acids, and by the AbrB protein. A genetic screen was devised to identify mu...

journal_title：Journal of bacteriology

authors： Slack FJ,Mueller JP,Sonenshein AL

更新日期：1993-08-01 00:00:00

abstract：:A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complement...

journal_title：Journal of bacteriology

authors： Cameron B,Guilhot C,Blanche F,Cauchois L,Rouyez MC,Rigault S,Levy-Schil S,Crouzet J

更新日期：1991-10-01 00:00:00

abstract：:The genes encoding ammonia monooxygenase (amoCAB), hydroxylamine oxidoreductase (hao), and the c-type cytochrome c-554 (hcy) are present in multiple copies in the genome of Nitrosomonas europaea. The upstream regions of the two copies of amoC, the three copies of hao, and one copy of hcy were cloned and sequenced. Pri...

journal_title：Journal of bacteriology

authors： Hommes NG,Sayavedra-Soto LA,Arp DJ

更新日期：2001-02-01 00:00:00

abstract：UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...

journal_title：Journal of bacteriology

authors： Compton CL,Fernandopulle MS,Nagari RT,Sello JK

更新日期：2015-09-01 00:00:00

abstract：:A mutant, cyaR1, affecting regulation of adenylate cyclase expression or activity is described. It was obtained as a thermoresistant revertant of a strain harboring a thermosensitive transcription termination factor, rho (rho-15). This mutant failed to synthesize adenosine 3',5'-phosphate and exhibited a carbohydrate-...

journal_title：Journal of bacteriology

authors： Guidi-Rontani C,Danchin A,Ullmann A

更新日期：1981-12-01 00:00:00

abstract：:Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture me...

journal_title：Journal of bacteriology

authors： Chisholm VT,Lea HZ,Rai R,Cooper TG

更新日期：1987-04-01 00:00:00

abstract：:Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...

journal_title：Journal of bacteriology

authors： Pannekoek H,Noordermeer I,van de Putte P

更新日期：1979-07-01 00:00:00

abstract：:A structural gene, valS, for the valyl-transfer ribonucleic acid synthetase of Escherichia coli has been mapped on the clockwise side of pyrB and is closely linked to it. ...

journal_title：Journal of bacteriology

authors： Tingle MA,Neidhardt FC

更新日期：1969-05-01 00:00:00

abstract：:The mutant sigA allele of Bacillus subtilis DB1005 was confirmed to be temperature sensitive (ts) and transferable among strains of B. subtilis by chromosomal transformation and gene conversion. This ts sigA allele had a pleiotropic effect on gene expression of DB1005. The induction of certain heat shock proteins in D...

journal_title：Journal of bacteriology

authors： Chang BY,Chen KY,Wen YD,Liao CT

更新日期：1994-06-01 00:00:00

abstract：:The interaction of 12 phage strains with bacilli, protoplasts, and L forms of Bacillus subtilis 168 and with eight of its mutants and two of its lysogens is described qualitatively and quantitatively. After removal of the cell wall from B. subtilis 168, 11 of the 12 phage strains can still adsorb to the protoplasts, n...

journal_title：Journal of bacteriology

authors： Jacobson ED,Landman OE

更新日期：1975-10-01 00:00:00

abstract：:Catheter-related bloodstream infections due to Staphylococcus aureus are of increasing clinical importance. The pathophysiological steps leading to colonization and infection, however, are still incompletely defined. We observed growth and detachment of S. aureus biofilms in an in vitro catheter-infection model by usi...

journal_title：Journal of bacteriology

authors： Fux CA,Wilson S,Stoodley P

更新日期：2004-07-01 00:00:00

abstract：:Chemotactic transducer proteins of Escherichia coli contain four or five methyl-accepting glutamates that are crucial for sensory adaptation and gradient sensing. Two residues arise from posttranslational deamidation of glutamines to yield methyl-accepting glutamates. We addressed the significance of this arrangement ...

journal_title：Journal of bacteriology

authors： Park C,Dutton DP,Hazelbauer GL

更新日期：1990-12-01 00:00:00

abstract：:The role of the carboxy terminus of the Escherichia coli cell division protein FtsA in bacterial division has been studied by making a series of short sequential deletions spanning from residue 394 to 420. Deletions as short as 5 residues destroy the biological function of the protein. Residue W415 is essential for th...

journal_title：Journal of bacteriology

authors： Yim L,Vandenbussche G,Mingorance J,Rueda S,Casanova M,Ruysschaert JM,Vicente M

更新日期：2000-11-01 00:00:00

abstract：:Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...

journal_title：Journal of bacteriology

authors： Grodberg J,Dunn JJ

更新日期：1988-03-01 00:00:00

abstract：:A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

journal_title：Journal of bacteriology

authors： Berg CM,Liu L,Wang B,Wang MD

更新日期：1988-01-01 00:00:00

abstract：:Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. Individual cell motility was ...

journal_title：Journal of bacteriology

authors： Hay NA,Tipper DJ,Gygi D,Hughes C

更新日期：1997-08-01 00:00:00

abstract：:In this report we present the first description of the isolation and partial characterization of the deoxyribonucleic acid (DNA) polymerase activity from two species of Mycoplasmatales, Mycoplasma orale type 1 and M. hyorhinis. We have identified only a single DNA polymerase species in the mycoplasma crude extracts, a...

journal_title：Journal of bacteriology

authors： Mills LB,Stanbridge EJ,Sedwick WD,Korn D

更新日期：1977-11-01 00:00:00

abstract：:The umuDC gene products, whose expression is induced by DNA-damaging treatments, have been extensively characterized for their role in SOS mutagenesis. We have recently presented evidence that supports a role for the umuDC gene products in the regulation of growth after DNA damage in exponentially growing cells, analo...

journal_title：Journal of bacteriology

authors： Murli S,Opperman T,Smith BT,Walker GC

更新日期：2000-02-01 00:00:00

abstract：:The roles of three TATA binding protein (TBP) homologs (TBP1, TBP2, and TBP3) in the archaeon Methanosarcina acetivorans were investigated by using genetic and molecular approaches. Although tbp2 and tbp3 deletion mutants were readily obtained, a tbp1 mutant was not obtained, and the growth of a conditional tbp1 expre...

journal_title：Journal of bacteriology

authors： Reichlen MJ,Murakami KS,Ferry JG

更新日期：2010-03-01 00:00:00