Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles.

abstract：:A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

journal_title：Journal of bacteriology

authors： Schecter JB

更新日期：1983-06-01 00:00:00

abstract：:We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These mutant strains were selected as glutamine-independent derivatives of a strain that is merodiploid for the glnA region and co...

journal_title：Journal of bacteriology

authors： Rothstein DM,Magasanik B

更新日期：1980-02-01 00:00:00

abstract：:Payne, W. J. (University of Georgia, Athens), and R. L. Todd. Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium. J. Bacteriol. 91:1533-1536. 1966.-Cell-free extracts of bacterium TEG-5 grown on tetraethylene glycol dehydrogenated a variety of ether glycols and nonylphenoxy and se...

journal_title：Journal of bacteriology

authors： Payne WJ,Todd RL

更新日期：1966-04-01 00:00:00

abstract：:Oxalurate, the gratuitous inducer of the allantoin degradative enzymes, was taken into the cell by an energy-dependent active transport system with an apparent Km of 1.2 mM. Efflux of previously accumulated oxalurate was rapid, with a half-life of about 2 min. The oxalurate uptake system appears to be both constitutiv...

journal_title：Journal of bacteriology

authors： Cooper TG,McKelvey J,Sumrada R

更新日期：1979-09-01 00:00:00

abstract：:The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...

journal_title：Journal of bacteriology

authors： Baxter BK,Craig EA

更新日期：1998-12-01 00:00:00

abstract：:The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...

journal_title：Journal of bacteriology

authors： Ying CW,Ordal GW

更新日期：1989-03-01 00:00:00

abstract：:The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related organism. S. thermophilus releases galactose into the medium during growth on lactose. Expulsion of galactose is mediated...

journal_title：Journal of bacteriology

authors： Lessard C,Cochu A,Lemay JD,Roy D,Vaillancourt K,Frenette M,Moineau S,Vadeboncoeur C

更新日期：2003-12-01 00:00:00

abstract：:The respiratory metabolism of Schizosaccharomyces pombe 972h(-), a fission, haplontic, "petite negative" yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions. l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth. d-Lactat...

journal_title：Journal of bacteriology

authors： Heslot H,Goffeau A,Louis C

更新日期：1970-10-01 00:00:00

abstract：:In the bacterium Escherichia coli, oxidized pyrimidines are removed by two DNA glycosylases, endonuclease III and endonuclease VIII (endo VIII), encoded by the nth and nei genes, respectively. Double mutants lacking both of these activities exhibit a high spontaneous mutation frequency, and here we show that all of th...

journal_title：Journal of bacteriology

authors： Blaisdell JO,Hatahet Z,Wallace SS

更新日期：1999-10-01 00:00:00

abstract：:The level of the vitamin B12 transport protein BtuB in the outer membrane of Escherichia coli is strongly reduced by growth in the presence of cobalamins. Previous analyses of regulatory mutants and of btuB-lacZ fusions indicated that the primary site of btuB gene regulation was at the translational level, and this re...

journal_title：Journal of bacteriology

authors： Nou X,Kadner RJ

更新日期：1998-12-01 00:00:00

abstract：:Two bacteriophage BF23 late genes, genes 24 and 25, were isolated on a 7.4-kb PstI fragment from the phage DNA, and their nucleotide sequences were determined. Gene 24 encodes a minor tail protein with the expected M(r) of 34,309, and gene 25 located 4 bp upstream of gene 24 encodes a major tail protein with the expec...

journal_title：Journal of bacteriology

authors： Nakayama S,Kaneko T,Ishimaru H,Moriwaki H,Mizobuchi K

更新日期：1994-12-01 00:00:00

abstract：:Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...

journal_title：Journal of bacteriology

authors： Cruz-Vera LR,New A,Squires C,Yanofsky C

更新日期：2007-04-01 00:00:00

abstract：:The Caulobacter crescentus DNA adenine methyltransferase CcrM and its homologs in the alpha-Proteobacteria are essential for viability. CcrM is 34% identical to the yhdJ gene products of Escherichia coli and Salmonella enterica. This study provides evidence that the E. coli yhdJ gene encodes a DNA adenine methyltransf...

journal_title：Journal of bacteriology

authors： Broadbent SE,Balbontin R,Casadesus J,Marinus MG,van der Woude M

更新日期：2007-06-01 00:00:00

abstract：:The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...

journal_title：Journal of bacteriology

authors： Jones HM,Gunsalus RP

更新日期：1985-12-01 00:00:00

abstract：:Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

journal_title：Journal of bacteriology

authors： Vogel AM,Das A

更新日期：1992-01-01 00:00:00

abstract：:SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids with...

journal_title：Journal of bacteriology

authors： Runti G,Lopez Ruiz Mdel C,Stoilova T,Hussain R,Jennions M,Choudhury HG,Benincasa M,Gennaro R,Beis K,Scocchi M

更新日期：2013-12-01 00:00:00

abstract：:The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs block...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ

更新日期：1974-06-01 00:00:00

abstract：:Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid homology, Exo53 has been shown to express ADP-ribosyltransferase activity at abou...

journal_title：Journal of bacteriology

authors： Liu S,Yahr TL,Frank DW,Barbieri JT

更新日期：1997-03-01 00:00:00

abstract：UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...

journal_title：Journal of bacteriology

authors： Fernicola S,Paiardini A,Giardina G,Rampioni G,Leoni L,Cutruzzolà F,Rinaldo S

更新日期：2015-09-28 00:00:00

abstract：:Mutation of a conserved His-157 in the second pore loop of KtrB drastically reduced the activity of the K+ transporter from Synechocystis sp. strain PCC 6803. This result suggests that His-157 plays an essential role in the K+ transport activity of the transporter system. ...

journal_title：Journal of bacteriology

authors： Zulkifli L,Uozumi N

更新日期：2006-11-01 00:00:00

abstract：:Surgalla, M. J. (Fort Detrick, Frederick, Md.), A. W. Andrews, and C. L. Baugh. Effects of bicarbonate on growth of Pasteurella pestis. I. Differential response of virulent and avirulent cells. J. Bacteriol. 88:269-272. 1964.-Virulent Pasteurella pestis and the avirulent mutants always present in a virulent inoculum h...

journal_title：Journal of bacteriology

authors： SURGALLA MJ,ANDREWS AW,BAUGH CL

更新日期：1964-08-01 00:00:00

abstract：:The regulation of Agrobacterium tumefaciens heat shock genes involves a transcriptional activator (RpoH) and repressor elements (HrcA-CIRCE). Using proteome analysis and mutants in these control elements, we show that the heat shock induction of 32 (out of 56) heat shock proteins is independent of RpoH and HrcA. These...

journal_title：Journal of bacteriology

authors： Rosen R,Büttner K,Becher D,Nakahigashi K,Yura T,Hecker M,Ron EZ

更新日期：2002-03-01 00:00:00

abstract：:Integral membrane proteins from over 20 ubiquitous families of channels, secondary carriers, and primary active transporters were analyzed for average size differences between homologues from the three domains of life: Bacteria, Archaea, and Eucarya. The results showed that while eucaryotic homologues are consistently...

journal_title：Journal of bacteriology

authors： Chung YJ,Krueger C,Metzgar D,Saier MH Jr

更新日期：2001-02-01 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious food-borne diseases in humans related to the consumption of raw seafood. It secretes a metalloprotease that is associated with skin lesions and serious hemorrhagic complications. In this study, we purified and characterized an extracellular metalloprotease (designated ...

journal_title：Journal of bacteriology

authors： Chang AK,Kim HY,Park JE,Acharya P,Park IS,Yoon SM,You HJ,Hahm KS,Park JK,Lee JS

更新日期：2005-10-01 00:00:00

abstract：:Partial denaturation of Saccharomyces cerevisiae chromosomal DNA was found to occur spontaneously during meiosis. Short regions of strand separation (300 base pairs long) were seen in DNA molecules prepared for electron microscopy by the aqueous spreading technique. These regions were clustered along the DNA. The time...

journal_title：Journal of bacteriology

authors： Klein HL,Byers B

更新日期：1978-05-01 00:00:00

abstract：:The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S...

journal_title：Journal of bacteriology

authors： Borges F,Layec S,Thibessard A,Fernandez A,Gintz B,Hols P,Decaris B,Leblond-Bourget N

更新日期：2005-04-01 00:00:00

abstract：:The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...

journal_title：Journal of bacteriology

authors： Ross TK,Achberger EC,Braymer HD

更新日期：1989-04-01 00:00:00

abstract：:Daniel, John W. (University of Wisconsin, Madison), and Karlee Babcock. Methionine metabolism of the myxomycete Physarum polycephalum. J. Bacteriol. 92:1028-1035. 1966.-Previous studies have shown that Physarum polycephalum requires exogenous methionine for growth, but not cysteine, folic acid, or vitamin B(12). Methi...

journal_title：Journal of bacteriology

authors： Daniel JW,Babcock K

更新日期：1966-10-01 00:00:00

abstract：:The phycocyanin subunit gene cluster is duplicated on the chromosome of the cyanobacterium Anacystis nidulans UTEX 625. The two gene clusters cpcB1A1 (left) and cpcB2A2 (right) are separated by about 2,500 base pairs, and in each cluster the beta-subunit gene is located upstream from the alpha-subunit gene. Filter hyb...

journal_title：Journal of bacteriology

authors： Kalla SR,Lind LK,Lidholm J,Gustafsson P

更新日期：1988-07-01 00:00:00

abstract：:Strain IMCC3088, cultivated from the Yellow Sea, is a novel isolate belonging to the OM60/NOR5 clade and is closely related to clone OM241, Congregibacter litoralis, and strain HTCC2080. Here, the genome sequence of strain IMCC3088 is presented, showing the absence of photosynthetic gene clusters and the presence of p...

journal_title：Journal of bacteriology

authors： Jang Y,Oh HM,Kang I,Lee K,Yang SJ,Cho JC

更新日期：2011-07-01 00:00:00