cse, a Chimeric and variable gene, encodes an extracellular protein involved in cellular segregation in Streptococcus thermophilus.

Abstract:

:The isolation of a Streptococcus thermophilus CNRZ368 mutant displaying a long-chain phenotype allowed us to identify the cse gene (for cellular segregation). The N terminus of Cse exhibits high similarity to Streptococcus agalactiae surface immunogenic protein (SIP), while its C terminus exhibits high similarity to S. thermophilus PcsB. In CNRZ368, deletion of the entire cse open reading frame leads to drastic lengthening of cell chains and altered colony morphology. Complementation of the Deltacse mutation with a wild-type allele restored both wild-type phenotypes. The central part of Cse is a repeat-rich region with low sequence complexity. Comparison of cse from CNRZ368 and LMG18311 strains reveals high variability of this repeat-rich region. To assess the impact of this central region variability, the central region of LMG18311 cse was exchanged with that of CNRZ368 cse. This replacement did not affect chain length, showing that divergence of the central part does not modify cell segregation activity of Cse. The structure of the cse locus suggests that the chimeric organization of cse results from insertion of a duplicated sequence deriving from the pcsB 3' end into an ancestral sip gene. Thus, the cse locus illustrates the module-shuffling mechanism of bacterial gene evolution.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Borges F,Layec S,Thibessard A,Fernandez A,Gintz B,Hols P,Decaris B,Leblond-Bourget N

doi

10.1128/JB.187.8.2737-2746.2005

keywords:

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

2737-46

issue

8

eissn

0021-9193

issn

1098-5530

pii

187/8/2737

journal_volume

187

pub_type

杂志文章
  • Difference between manganese ion requirements of pediococci and enterococci.

    abstract::For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.627-628.1972

    authors: Efthymiou CJ,Joseph SW

    更新日期:1972-10-01 00:00:00

  • Inhibition of peptidoglycan biosynthesis at a postcytoplasmic reaction in a stable L-phase variant of Streptococcus faecium.

    abstract::Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.900-910.1978

    authors: Gregory WW,Gooder H

    更新日期:1978-09-01 00:00:00

  • Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci.

    abstract::Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.2.169-180.1961

    authors: FIRSHEIN W

    更新日期:1961-08-01 00:00:00

  • Purification and properties of Myxococcus xanthus cell surface antigen 1604.

    abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4655-4666.1989

    authors: Jarvis BW,Dworkin M

    更新日期:1989-09-01 00:00:00

  • Protein-tyrosine phosphorylation in the Archaea.

    abstract::Sulfolobus sulfataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryarchaeota and Crenarchaeota subdomains of the Archaea, contain proteins which are phosphorylated on tyrosine. These data raise fundamental questions as to the origin and evolution of tyrosine phosphorylati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.7.2418-2420.1997

    authors: Smith SC,Kennelly PJ,Potts M

    更新日期:1997-04-01 00:00:00

  • Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).

    abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.416-421.1975

    authors: Schrempf H,Bujard H,Hopwood DA,Goebel W

    更新日期:1975-02-01 00:00:00

  • Detection and quantification of superoxide formed within the periplasm of Escherichia coli.

    abstract::Many gram-negative bacteria harbor a copper/zinc-containing superoxide dismutase (CuZnSOD) in their periplasms. In pathogenic bacteria, one role of this enzyme may be to protect periplasmic biomolecules from superoxide that is released by host phagocytic cells. However, the enzyme is also present in many nonpathogens ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00554-06

    authors: Korshunov S,Imlay JA

    更新日期:2006-09-01 00:00:00

  • Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.

    abstract::The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2707-2716.1997

    authors: Schäferkordt S,Chakraborty T

    更新日期:1997-04-01 00:00:00

  • Functional interactions between coexisting toxin-antitoxin systems of the ccd family in Escherichia coli O157:H7.

    abstract::Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd(F)) located in the chromosomal backbone of the pathogenic O157:H7 Es...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01679-06

    authors: Wilbaux M,Mine N,Guérout AM,Mazel D,Van Melderen L

    更新日期:2007-04-01 00:00:00

  • Response of Bacillus subtilis to cerulenin and acquisition of resistance.

    abstract::Cerulenin is a fungal mycotoxin that potently inhibits fatty acid synthesis by covalent modification of the active site thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein (ACP) synthases. The Bacillus subtilis fabF (yjaY) gene (fabF(b)) encodes an enzyme that catalyzes the condensation of mal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.10.3032-3040.2001

    authors: Schujman GE,Choi KH,Altabe S,Rock CO,de Mendoza D

    更新日期:2001-05-01 00:00:00

  • Organization of the genes for protein synthesis elongation factors Tu and G in the cyanobacterium Anacystis nidulans.

    abstract::The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.1.78-82.1986

    authors: Mickel FS,Spremulli LL

    更新日期:1986-04-01 00:00:00

  • Inhibition of inducible liver enzymes by endotoxin and actinomycin D.

    abstract::Berry, L. Joe (Bryn Mawr College, Bryn Mawr, Pa.), Dorothy S. Smythe, and Louise S. Colwell. Inhibition of inducible liver enzymes by endotoxin and actinomycin D. J. Bacteriol. 92:107-115. 1966.-Bacterial endotoxin at the ld(50) level lowers liver tryptophan pyrrolase in mice, it prevents for 16 to 20 hr the induction...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.1.107-115.1966

    authors: Berry LJ,Smythe DS,Colwell LS

    更新日期:1966-07-01 00:00:00

  • YkdA and YvtA, HtrA-like serine proteases in Bacillus subtilis, engage in negative autoregulation and reciprocal cross-regulation of ykdA and yvtA gene expression.

    abstract::HtrA-type serine proteases participate in folding and degradation of aberrant proteins and in processing and maturation of native proteins. Mutation of the corresponding genes often confers a pleiotropic phenotype that can include temperature sensitivity, sensitivity to osmotic and oxidative stress, and attenuated vir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.2.654-663.2001

    authors: Noone D,Howell A,Collery R,Devine KM

    更新日期:2001-01-01 00:00:00

  • Deoxyribonucleic acid characterization of Bdellovibrios.

    abstract::The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.786-790.1969

    authors: Seidler RJ,Starr MP,Mandel M

    更新日期:1969-11-01 00:00:00

  • Population studies of methicillin-resistant and -sensitive Staphylococcus aureus strains reveal a lack of variability in the agrD gene, encoding a staphylococcal autoinducer peptide.

    abstract::The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.20.5721-5729.2000

    authors: van Leeuwen W,van Nieuwenhuizen W,Gijzen C,Verbrugh H,van Belkum A

    更新日期:2000-10-01 00:00:00

  • DNA-damaging agents induce the RecA-independent homologous recombination functions of integrating conjugative elements of the SXT/R391 family.

    abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family are major contributors to the spread of antibiotic resistance genes. These elements also catalyze their own diversity by promoting inter-ICE recombination through the action of the RecA-independent homologous recombination system that they encode. Here, we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02090-12

    authors: Garriss G,Poulin-Laprade D,Burrus V

    更新日期:2013-05-01 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • DNA microarray and proteomic analyses of the RpoS regulon in Geobacter sulfurreducens.

    abstract::The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.2792-2800.2006

    authors: Núñez C,Esteve-Núñez A,Giometti C,Tollaksen S,Khare T,Lin W,Lovley DR,Methé BA

    更新日期:2006-04-01 00:00:00

  • Crystal structure of PhnF, a GntR-family transcriptional regulator of phosphate transport in Mycobacterium smegmatis.

    abstract::Bacterial uptake of phosphate is usually accomplished via high-affinity transporters that are commonly regulated by two-component systems, which are activated when the concentration of phosphate is low. Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transpo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01965-14

    authors: Gebhard S,Busby JN,Fritz G,Moreland NJ,Cook GM,Lott JS,Baker EN,Money VA

    更新日期:2014-10-01 00:00:00

  • Methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A and its repressibility by serine.

    abstract::The methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A(k) was purified 100-fold. Because it is extremely labile, this enzyme required protection by 1 mm nicotinamide adenine dinucleotide phosphate (NADP(+)) during purification; 0.01 mm EADP(+) with 0.1% bov...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1779-1789.1968

    authors: Albrecht AM,Pearce FK,Hutchison DJ

    更新日期:1968-05-01 00:00:00

  • The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system.

    abstract::The ftf gene, coding for the cell-bound beta-D-fructosyltransferase (FTF) of Streptococcus salivarius ATCC 25975, has been analyzed, and its deduced amino acid sequence has been compared with that of the secreted FTF of Streptococcus mutans and the levansucrases (SacBs) of Bacillus species. A unique proline-rich regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.14.4520-4527.1993

    authors: Rathsam C,Giffard PM,Jacques NA

    更新日期:1993-07-01 00:00:00

  • Lactate metabolism by Veillonella parvula.

    abstract::A strain of Veillonella parvula M4, which grows readily in lactate broth without a requirement for carbon dioxide, has been isolated from the oral cavity. Anaerobic, washed cells of this organism fermented sodium lactate to the following products (moles/100 moles of lactate): propionate, 66; acetate, 40; carbon dioxid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.999-1005.1971

    authors: Ng SK,Hamilton IR

    更新日期:1971-03-01 00:00:00

  • Plasmid-determined enzymatic degradation of nylon oligomers.

    abstract::The nylon oligomer (6-aminohexanoic acid cyclic dimer) degradation genes on plasmid pOAD2 of Flavobacterium sp. KI72 were cloned into Escherichia coli vector pBR322. The locus of one of the genes, the structural gene of 6-aminohexanoic acid linear oligomer hydrolase, was determined by constructing various deletion pla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.1.22-31.1983

    authors: Negoro S,Taniguchi T,Kanaoka M,Kimura H,Okada H

    更新日期:1983-07-01 00:00:00

  • Regulation of derepressed synthesis of arylsulfatase by tyramine oxidase in Salmonella typhimurium.

    abstract::The participation of tyramine oxidase in the regulation of arylsulfatase synthesis in Salmonella typhimurium was studied. Arylsulfatase synthesis was repressed by inorganic sulfate, cysteine, methionine, or taurine. This repression was relieved by tyramine, octopamine, or dopamine, which induced tyramine oxidase synth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.796-802.1981

    authors: Murooka Y,Harada T

    更新日期:1981-02-01 00:00:00

  • Role of lithium ions in proline transport in Escherichia coli.

    abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.560-564.1979

    authors: Kayama-Gonda Y,Kawasaki T

    更新日期:1979-08-01 00:00:00

  • Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

    abstract::With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.492-500.1977

    authors: Potter SS,Bott KF,Newbold JE

    更新日期:1977-01-01 00:00:00

  • Unsaturated fatty acid mutants of Neurospora crassa.

    abstract::Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.1144-1148.1977

    authors: Scott WA

    更新日期:1977-06-01 00:00:00

  • Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.

    abstract::The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2626-2633.1989

    authors: Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

    更新日期:1989-05-01 00:00:00

  • The linker region of AraC protein.

    abstract::AraC protein, a transcriptional regulator of the L-arabinose operon in Escherichia coli, is dimeric. Each monomer consists of a domain for DNA binding plus transcription activation and a domain for dimerization plus arabinose binding. These are connected to one another by a linker region of at least 5 amino acids. Her...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.24.7025-7030.1996

    authors: Eustance RJ,Schleif RF

    更新日期:1996-12-01 00:00:00

  • The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro.

    abstract::The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.5.1364-1373.2000

    authors: Lafontaine ER,Cope LD,Aebi C,Latimer JL,McCracken GH Jr,Hansen EJ

    更新日期:2000-03-01 00:00:00