Isolation of covalently closed circular deoxyribonucleic acid from Streptomyces coelicolor A3(2).

abstract：:Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

journal_title：Journal of bacteriology

authors： De Boer WR,Kruyssen FJ,Wouters JT

更新日期：1981-01-01 00:00:00

abstract：:Morphogenesis of the filamentous bacteriophage f1 occurred at adhesion zones between the inner and outer membranes of the host cell. Quantitation of adhesion zones in cells infected with mutant phage strains suggested that the phage gene I protein may be involved in the formation of adhesion zones for phage assembly. ...

journal_title：Journal of bacteriology

authors： Lopez J,Webster RE

更新日期：1985-09-01 00:00:00

abstract：:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

journal_title：Journal of bacteriology

authors： Jin H,Nikolau BJ

更新日期：2012-10-01 00:00:00

abstract：:Streptococcus faecalis strain JH1 harbors two conjugative plasmids: pJH1, an R plasmid mediating resistance to kanamycin, streptomycin, gentamicin, erythromycin, and tetracycline, and pJH2, a hemolysin-bacteriocin plasmid. Studies of plasmid-cured derivatives of strain JH1 and of transconjugates obtained after mixed i...

journal_title：Journal of bacteriology

authors： LeBlanc DJ,Lee LN

更新日期：1982-05-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these c...

journal_title：Journal of bacteriology

authors： Miranda-Casoluengo R,Duffy PS,O'Connell EP,Graham BJ,Mangan MW,Prescott JF,Meijer WG

更新日期：2005-05-01 00:00:00

abstract：:A general, genetic technique for determining the direction of transcription for bacterial genes is presented. By comparing the phenotype of Mu-Mud1 dilysogens with the phenotype of deletion-containing derivatives, the direction of transcription for the gene containing Mud1 can be unambiguously determined. This method ...

journal_title：Journal of bacteriology

authors： MacNeil D

更新日期：1981-04-01 00:00:00

abstract：:In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254. ...

journal_title：Journal of bacteriology

authors： Kunnimalaiyaan S,Rakowski SA,Filutowicz M

更新日期：2007-07-01 00:00:00

abstract：:We identified in Listeria monocytogenes a gene encoding a protein homologous to MecA, a regulatory protein acting with ClpC and ComK in the competence pathway of Bacillus subtilis. In L. monocytogenes, MecA is involved, along with ClpC and ClpP, in the downregulation of a 64-kDa secreted protein. In B. subtilis, the M...

journal_title：Journal of bacteriology

authors： Borezee E,Msadek T,Durant L,Berche P

更新日期：2000-10-01 00:00:00

abstract：:Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of un...

journal_title：Journal of bacteriology

authors： Waldo RH 3rd,Popham PL,Romero-Arroyo CE,Mothershed EA,Lee KK,Krause DC

更新日期：1999-08-01 00:00:00

abstract：:Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...

journal_title：Journal of bacteriology

authors： Huff E

更新日期：1982-01-01 00:00:00

abstract：:Coscarelli, Waldimero (Rutgers, The State University, New Brunswick, N.J.) and David Pramer. Nutrition and growth of Arthrobotrys conoides. J. Bacteriol. 84:60-64. 1962-Despite its ability to capture, kill, and subsist on nematodes, the predacious fungus Arthrobotrys conoides does not demonstrate a unique nutritional ...

journal_title：Journal of bacteriology

authors： COSCARELLI W,PRAMER D

更新日期：1962-07-01 00:00:00

abstract：:Eisenberg, M. A. (Columbia University, New York, N.Y.). Biotin biosynthesis. I. Biotin yields and biotin vitamers in cultures of Phycomyces blakesleeanus. J. Bacteriol. 86:673-680. 1963.-The addition of pimelic acid to a well-aerated medium resulted in a 10- to 12-fold increase in the biotin production of Phycomyces b...

journal_title：Journal of bacteriology

authors： EISENBERG MA

更新日期：1963-10-01 00:00:00

abstract：:Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...

journal_title：Journal of bacteriology

authors： Hales LM,Gumport RI,Gardner JF

更新日期：1994-05-01 00:00:00

abstract：:E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...

journal_title：Journal of bacteriology

authors： Krüger N,Oppermann FB,Lorenzl H,Steinbüchel A

更新日期：1994-06-01 00:00:00

abstract：:Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

journal_title：Journal of bacteriology

authors： Flannagan SE,Clewell DB

更新日期：1991-11-01 00:00:00

abstract：:Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

journal_title：Journal of bacteriology

authors： Costello GM,Vipond R,MacIntyre S

更新日期：1996-03-01 00:00:00

abstract：:PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

journal_title：Journal of bacteriology

authors： Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

更新日期：2009-05-01 00:00:00

abstract：:A group of enzymes of sulfur metabolism (arylsulfatase, cholinesulfatase, and a number of others) are normally repressed in Neurospora crassa by an abundant supply of a "favored" sulfur source such as methionine or inorganic sulfate. A mutant called scon(c) was isolated in which the formation of each of these enzymes ...

journal_title：Journal of bacteriology

authors： Burton EG,Metzenberg RL

更新日期：1972-01-01 00:00:00

abstract：:Cyclic nucleotide-independent protein kinase (EC 2.7.1.37) activity was found in the nuclear cap organelle, within which ribosomes of zoospores of Blastocladiella emersonii are sequestered. Two protein kinase activities were resolved from the high-salt wash fraction of zoospore ribosomes by selective adsorption to DEA...

journal_title：Journal of bacteriology

authors： Bonato MC,da Costa Maia JC,Juliani MH

更新日期：1983-06-01 00:00:00

abstract：:Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...

journal_title：Journal of bacteriology

authors： Futai M

更新日期：1974-11-01 00:00:00

abstract：:Despite the considerable knowledge of bacterial high-molecular-weight (HMW) polycyclic aromatic hydrocarbon (PAH) metabolism, the key enzyme(s) and its pleiotropic and epistatic behavior(s) responsible for low-molecular-weight (LMW) PAHs in HMW PAH-metabolic networks remain poorly understood. In this study, a phenotyp...

journal_title：Journal of bacteriology

authors： Kweon O,Kim SJ,Kim DW,Kim JM,Kim HL,Ahn Y,Sutherland JB,Cerniglia CE

更新日期：2014-10-01 00:00:00

abstract：:In cyanobacteria, gene expression is regulated mainly at the level of transcription initiation, which is mediated by the RNA polymerase holoenzyme. The RNA polymerase core is catalytically active, while the sigma factor recognizes promoter sequences. Group 2 sigma factors are similar to the principal sigma factor but ...

journal_title：Journal of bacteriology

authors： Pollari M,Ruotsalainen V,Rantamäki S,Tyystjärvi E,Tyystjärvi T

更新日期：2009-06-01 00:00:00

abstract：:Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...

journal_title：Journal of bacteriology

authors： Okanishi M,Gregory KF

更新日期：1970-12-01 00:00:00

abstract：:Two genes, xylP and xylQ, from the xylose regulon of Lactobacillus pentosus were cloned and sequenced. Together with the repressor gene of the regulon, xylR, the xylPQ genes form an operon which is inducible by xylose and which is transcribed from a promoter located 145 bp upstream of xylP. A putative xylR binding sit...

journal_title：Journal of bacteriology

authors： Chaillou S,Lokman BC,Leer RJ,Posthuma C,Postma PW,Pouwels PH

更新日期：1998-05-01 00:00:00

abstract：:Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous an...

journal_title：Journal of bacteriology

authors： Neubauer C,Kasi AS,Grahl N,Sessions AL,Kopf SH,Kato R,Hogan DA,Newman DK

更新日期：2018-11-26 00:00:00

abstract：:One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppres...

journal_title：Journal of bacteriology

authors： Blanc-Potard AB,Bossi L

更新日期：1994-04-01 00:00:00

abstract：:Archaea, constituting the third primary domain of life, harbor a basal transcription apparatus of the eukaryotic type, whereas curiously, a large fraction of the potential transcription regulation factors appear to be of the bacterial type. To date, little information is available on these predicted regulators and on ...

journal_title：Journal of bacteriology

authors： Enoru-Eta J,Gigot D,Thia-Toong TL,Glansdorff N,Charlier D

更新日期：2000-07-01 00:00:00

abstract：:The development of competence by the dental caries pathogen Streptococcus mutans is mediated primarily through the alternative sigma factor ComX (SigX), which is under the control of multiple regulatory systems and activates the expression of genes involved in DNA uptake and recombination. Here we report that the indu...

journal_title：Journal of bacteriology

authors： Guo Q,Ahn SJ,Kaspar J,Zhou X,Burne RA

更新日期：2014-01-01 00:00:00

abstract：:Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...

journal_title：Journal of bacteriology

authors： Leu YL,Wang PH,Shiao MS,Ismail W,Chiang YR

更新日期：2011-09-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00