Cloning, sequence analysis, and characterization of the genes involved in isoprimeverose metabolism in Lactobacillus pentosus.

abstract：:Heterocyst development was analyzed in mutants of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 bearing inactivated cox2 and/or cox3 genes, encoding heterocyst-specific terminal respiratory oxidases. At the morphological level, the cox2 cox3 double mutant (strain CSAV141) was impaired in membrane ...

journal_title：Journal of bacteriology

authors： Valladares A,Maldener I,Muro-Pastor AM,Flores E,Herrero A

更新日期：2007-06-01 00:00:00

abstract：:An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

journal_title：Journal of bacteriology

authors： Gardner RM,Doerner KC,White BA

更新日期：1987-10-01 00:00:00

abstract：:Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

journal_title：Journal of bacteriology

authors： Gilden RV,Carp RI

更新日期：1966-03-01 00:00:00

abstract：:The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that...

journal_title：Journal of bacteriology

authors： Saito Y,Uraki F,Nakajima S,Asaeda A,Ono K,Kubo K,Yamamoto K

更新日期：1997-06-01 00:00:00

abstract：:Transcriptional fusions to Salmonella typhimurium chromosomal genes were constructed by integration of a suicide fusion vector into the chromosome by homologous recombination with random cloned chromosomal fragments. We describe here a transductional method using the generalized transducing phage of S. typhimurium, P2...

journal_title：Journal of bacteriology

authors： Mahan MJ,Slauch JM,Mekalanos JJ

更新日期：1993-11-01 00:00:00

abstract：:Spirochete MA-2, which is anaerobic, ferments glucose, forming acetate as a major product. The spirochete also ferments (but does not utilize as growth substrates) small amounts of l-leucine, l-isoleucine, and l-valine, forming the branched-chain fatty acids isovalerate, 2-methylbutyrate, and isobutyrate, respectively...

journal_title：Journal of bacteriology

authors： Harwood CS,Canale-Parola E

更新日期：1982-10-01 00:00:00

abstract：:Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...

journal_title：Journal of bacteriology

authors： Grunberg E,Cleeland R

更新日期：1966-07-01 00:00:00

abstract：:Escherichia coli K-12 deficient in galactose-1-phosphate uridyl transferase is capable of converting significant amounts of d-[1-(14)C]galactose to (14)CO(2), whereas strains deficient in other enzymes of the Leloir pathway cannot do so. ...

journal_title：Journal of bacteriology

authors： LaPolla RJ,Geier MR,Friedman TB,Merril CR

更新日期：1975-10-01 00:00:00

abstract：UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...

journal_title：Journal of bacteriology

authors： Tawk CS,Ghattas IR,Smith CA

更新日期：2015-11-01 00:00:00

abstract：:Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...

journal_title：Journal of bacteriology

authors： Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

更新日期：2008-11-01 00:00:00

abstract：:The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A mutant gshB allele ...

journal_title：Journal of bacteriology

authors： Daws T,Lim CJ,Fuchs JA

更新日期：1989-09-01 00:00:00

abstract：:The TspO outer membrane protein of Rhodobacter sphaeroides has been shown to be involved in controlling the transcription of a number of genes which encode enzymes involved in photopigment biosynthesis and the puc operon. The display of regulated genes appears identical to those genes encompassing the PpsR/AppA repres...

journal_title：Journal of bacteriology

authors： Zeng X,Kaplan S

更新日期：2001-11-01 00:00:00

abstract：:In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcri...

journal_title：Journal of bacteriology

authors： Iuchi S,Kuritzkes DR,Lin EC

更新日期：1985-03-01 00:00:00

abstract：:Expression of the Escherichia coli proU operon, which encodes an efficient uptake system for the osmoprotectant glycine betaine, is strongly increased in cells grown at high osmolarity. We isolated 182 independent spontaneous mutants with elevated expression of the chromosomal phi(proV-lacZ) (Hyb2) fusion at low osmol...

journal_title：Journal of bacteriology

authors： Lucht JM,Bremer E

更新日期：1991-01-01 00:00:00

abstract：:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

journal_title：Journal of bacteriology

authors： Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

更新日期：1993-10-01 00:00:00

abstract：:The bacteriophage lambda cIII gene product regulates the lysogenic pathway. The cIII gene is located in the leftward operon, which is transcribed from the pL promoter. We have previously shown (S. Altuvia and A. B. Oppenheim, J. Bacteriol. 167:415-419, 1986) that mutations that show elevated expression lie within the ...

journal_title：Journal of bacteriology

authors： Kornitzer D,Teff D,Altuvia S,Oppenheim AB

更新日期：1989-05-01 00:00:00

abstract：:Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

journal_title：Journal of bacteriology

authors： Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

更新日期：2005-03-01 00:00:00

abstract：:Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...

journal_title：Journal of bacteriology

authors： Day LE

更新日期：1966-07-01 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:The Escherichia coli plasmids pBR322 and pBR327 can be taken up by Haemophilus influenzae but do not replicate in this organism; however, integration of pBR into the H. influenzae chromosome was achieved by ligation to a fragment of the Haemophilus phage S2 that carried a phage attachment site (attP). Once these seque...

journal_title：Journal of bacteriology

authors： Kauc L,Goodgal SH

更新日期：1989-04-01 00:00:00

abstract：:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

journal_title：Journal of bacteriology

authors： Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

更新日期：1972-03-01 00:00:00

abstract：:Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

journal_title：Journal of bacteriology

authors： Brandriss MC

更新日期：1979-06-01 00:00:00

abstract：:Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

journal_title：Journal of bacteriology

authors： Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

更新日期：2008-05-01 00:00:00

abstract：:In Mexico, actinomycetoma is mainly caused by Nocardia brasiliensis, which is a soil inhabitant actinobacterium. Here, we report for the first time the draft genome of a strain isolated from a human case that has largely been found in in vitro and experimental models of actinomycetoma, N. brasiliensis HUJEG-1. ...

journal_title：Journal of bacteriology

authors： Vera-Cabrera L,Ortiz-Lopez R,Elizondo-Gonzalez R,Perez-Maya AA,Ocampo-Candiani J

更新日期：2012-05-01 00:00:00

abstract：:Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...

journal_title：Journal of bacteriology

authors： Sadaie Y,Kada T

更新日期：1985-08-01 00:00:00

abstract：:An IMP dehydrogenase gene was isolated from Candida albicans on a approximately 2.9-kb XbaI genomic DNA fragment. The putative Candida IMP dehydrogenase gene (IMH3) encodes a protein of 521 amino acids with extensive sequence similarity to the IMP dehydrogenases of Saccharomyces cerevisiae and various other organisms....

journal_title：Journal of bacteriology

authors： Köhler GA,White TC,Agabian N

更新日期：1997-04-01 00:00:00

abstract：:Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxan...

journal_title：Journal of bacteriology

authors： Garzón A,Li J,Flores A,Casadesus J,Stewart V

更新日期：1992-10-01 00:00:00

abstract：:Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...

journal_title：Journal of bacteriology

authors： Allaoui A,Scheen R,Lambert de Rouvroit C,Cornelis GR

更新日期：1995-08-01 00:00:00

abstract：:The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synth...

journal_title：Journal of bacteriology

authors： Derman AI,Beckwith J

更新日期：1991-12-01 00:00:00

abstract：:Sinorhizobium meliloti can live as a soil saprophyte and can engage in a nitrogen-fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma (σ) factors interacting wit...

journal_title：Journal of bacteriology

authors： Barnett MJ,Bittner AN,Toman CJ,Oke V,Long SR

更新日期：2012-09-01 00:00:00