Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA.

Abstract:

:Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both genes ompAI and ompAII) selected by immunoscreening. A 5.0-kb BamHI fragment containing the two genes in tandem was subcloned in pBluescript and used for further subcloning and sequencing of the genes. The encoded proteins (Mr = 33,564 and 32,536 for mature OmpAI and OmpAII, respectively) had only 64% identity with each other and otherwise had the highest level of homology to OmpA proteins from the members of the family Enterobacteriaceae. Based on the Escherichia coli OmpA model, an eight-stranded amphipathic beta-barrel model for the membrane assembly of the N-terminal half of OmpAI and OmpAII was predicted. Most variation between the two proteins was localized to the predicted surface loops and periplasmic turns, while the transmembrane strands and C-terminals domains were highly conserved. Expression of ompAI and ompAII separately in E. coli indicated that both genes could be independently transcribed from their own promoters and that both gene products were assembled into the E. coli outer membrane. A survey of different Aeromonas spp. by PCR revealed that possession of two tandem ompA genes was widespread among this genus. This is the first report of any bacterial species possessing two genes for homologs of this major outer membrane protein.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Costello GM,Vipond R,MacIntyre S

doi

10.1128/jb.178.6.1623-1630.1996

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

1623-30

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Cell growth inhibition upon deletion of four toxin-antitoxin loci from the megaplasmids of Sinorhizobium meliloti.

    abstract::Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01104-13

    authors: Milunovic B,diCenzo GC,Morton RA,Finan TM

    更新日期:2014-02-01 00:00:00

  • Functional analysis of OleY L-oleandrosyl 3-O-methyltransferase of the oleandomycin biosynthetic pathway in Streptomyces antibioticus.

    abstract::Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.183.18.5358-5363.2001

    authors: Rodríguez L,Rodríguez D,Olano C,Braña AF,Méndez C,Salas JA

    更新日期:2001-09-01 00:00:00

  • Protein synthesis during fungal spore germination. II. Aminoacyl-soluble ribonucleic acid synthetase activities during germination of Botryodiplodia theobromae spores.

    abstract::The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1042-1048.1968

    authors: Van Etten JL,Brambl RM

    更新日期:1968-10-01 00:00:00

  • Genetic control of the rate of alpha-amylase synthesis in Bacillus subtilis.

    abstract::The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.2.410-415.1974

    authors: Yamaguchi K,Nagata Y,Maruo B

    更新日期:1974-08-01 00:00:00

  • Transformation of leucine and rifampin traits in Neisseria gonorrhoeae with deoxyribonucleic acid from homologous and heterologous origins.

    abstract::A leucine-requiring, rifampin-sensitive strain of Neisseria gonorrhoeae was transformed to a leucine-nonrequiring, rifampin-resistant phenotype with deoxyribonucleic acid (DNA) obtained from both N. meningitidis and N. gonorrhoeae. The transforming efficiency of the meningococcal DNA was about 10- to 100-fold less tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.471-474.1975

    authors: Wood DO,Brownell GH

    更新日期:1975-02-01 00:00:00

  • Transient Intestinal Colonization by a Live-Attenuated Oral Cholera Vaccine Induces Protective Immune Responses in Streptomycin-Treated Mice.

    abstract::Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00232-20

    authors: Fakoya B,Sit B,Waldor MK

    更新日期:2020-11-19 00:00:00

  • Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli.

    abstract::The phs mutation of Escherichia coli has been suggested to affect the Na+/H+ antiport (D. Zilberstein, E. Padan, and S. Schuldiner, FEBS Lett. 168:327-330, 1980). We have recently shown that the mutation affects the rpoA gene and thus affects transcription. The extent of the pleiotropy of the phs mutation was investig...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.904-910.1985

    authors: Giffard PM,Rowland GC,Kroll RG,Stewart LM,Bakker EP,Booth IR

    更新日期:1985-11-01 00:00:00

  • Selectivity among Anti-σ Factors by Mycobacterium tuberculosis ClpX Influences Intracellular Levels of Extracytoplasmic Function σ Factors.

    abstract::Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00748-18

    authors: Joshi AC,Kaur P,Nair RK,Lele DS,Nandicoori VK,Gopal B

    更新日期:2019-02-25 00:00:00

  • A dominant-negative fur mutation in Bradyrhizobium japonicum.

    abstract::In many bacteria, the ferric uptake regulator (Fur) protein plays a central role in the regulation of iron uptake genes. Because iron figures prominently in the agriculturally important symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, we wanted to assess the role of Fur in the i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1409-1414.2004

    authors: Benson HP,LeVier K,Guerinot ML

    更新日期:2004-03-01 00:00:00

  • EARLY EFFECTS OF STREPTOMYCIN ON BACILLUS MEGATERIUM.

    abstract::Hancock, R. (Harvard Medical School, Boston, Mass.). Early effects of streptomycin on Bacillus megaterium. J. Bacteriol. 88:633-639. 1964.-The sequence of some early effects of streptomycin on Bacillus megaterium was investigated. An increase in the rate of efflux of cellular K ions preceded loss of viability, but eff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.3.633-639.1964

    authors: HANCOCK R

    更新日期:1964-09-01 00:00:00

  • Targeting the Achilles' heel of bacteria: different mechanisms to break down the peptidoglycan cell wall during bacterial warfare.

    abstract::Bacteria commonly live in dense polymicrobial communities and compete for scarce resources. Consequently, they employ a diverse array of mechanisms to harm, inhibit and kill their competitors. The cell wall is essential for bacterial survival by providing mechanical strength to resist osmotic stress. Because peptidogl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00478-20

    authors: Sibinelli-Sousa S,Hespanhol JT,Bayer-Santos E

    更新日期:2020-11-02 00:00:00

  • HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum.

    abstract::Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00071-12

    authors: Escamilla-Hernandez R,O'Brian MR

    更新日期:2012-06-01 00:00:00

  • Coupling of asymmetric division to polar placement of replication origin regions in Bacillus subtilis.

    abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.13.4052-4060.2001

    authors: Graumann PL,Losick R

    更新日期:2001-07-01 00:00:00

  • Sequence diversity of Treponema pallidum subsp. pallidum tprK in human syphilis lesions and rabbit-propagated isolates.

    abstract::The tprK gene of Treponema pallidum subsp. pallidum, the causative agent of venereal syphilis, belongs to a 12-member gene family and encodes a protein with a predicted cleavable signal sequence and predicted transmembrane domains. Except for the Nichols type strain, all rabbit-propagated isolates of T. pallidum exami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.21.6262-6268.2003

    authors: LaFond RE,Centurion-Lara A,Godornes C,Rompalo AM,Van Voorhis WC,Lukehart SA

    更新日期:2003-11-01 00:00:00

  • In vitro construction of gshB::kan in Escherichia coli and use of gshB::kan in mapping the gshB locus.

    abstract::The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A mutant gshB allele ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.5218-5221.1989

    authors: Daws T,Lim CJ,Fuchs JA

    更新日期:1989-09-01 00:00:00

  • Bacteriophage T4 self-assembly: in vitro reconstitution of recombinant gp2 into infectious phage.

    abstract::T4 gene 2 mutants have a pleiotropic phenotype: degradation of injected phage DNA by exonuclease V (ExoV) in the recBCD(+) host cell cytoplasm and a low burst size due, at least in part, to a decreased ability for head-to-tail (H-T) joining. The more N terminal the mutation, the more pronounced is the H-T joining defe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.672-679.2000

    authors: Wang GR,Vianelli A,Goldberg EB

    更新日期:2000-02-01 00:00:00

  • Identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract::In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purified mutant enzymes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2602-2605.1995

    authors: Kim SW,Choi KY

    更新日期:1995-05-01 00:00:00

  • The lcrB (yscN/U) gene cluster of Yersinia pseudotuberculosis is involved in Yop secretion and shows high homology to the spa gene clusters of Shigella flexneri and Salmonella typhimurium.

    abstract::Virulent bacteria of the genus Yersinia secrete a number of virulence determinants called Yops. These proteins lack typical signal sequences and are not posttranslationally processed. Two gene loci have been identified as being involved in the specific Yop secretion system (G. Cornelis, p. 231-265, In C. E. Hormache, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.9.2619-2626.1994

    authors: Bergman T,Erickson K,Galyov E,Persson C,Wolf-Watz H

    更新日期:1994-05-01 00:00:00

  • Characterization of genes for synthesis and catabolism of a new rhizopine induced in nodules by Rhizobium meliloti Rm220-3: extension of the rhizopine concept.

    abstract::Rhizopines are selective growth substrates synthesized in nodules only by strains of rhizobia capable of their catabolism. We report the isolation and study of genes for the synthesis and catabolism of a new rhizopine, scyllo-inosamine (sIa), from alfalfa nodules induced by Rhizobium meliloti Rm220-3. This compound is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5205-5215.1993

    authors: Saint CP,Wexler M,Murphy PJ,Tempé J,Tate ME,Murphy PJ

    更新日期:1993-08-01 00:00:00

  • A new pathway for salvaging the coenzyme B12 precursor cobinamide in archaea requires cobinamide-phosphate synthase (CbiB) enzyme activity.

    abstract::The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.24.7193-7201.2003

    authors: Woodson JD,Zayas CL,Escalante-Semerena JC

    更新日期:2003-12-01 00:00:00

  • Factors affecting growth and nitrogen fixation of Spirillum lipoferum.

    abstract::Spirillum lipoferum grows vigorously on malate, succinate, lactate, or pyruvate, moderately on galactose or acetate, and poorly on glucose or citrate. It reduces 15N2. Acetylene reduction rates decrease rapidly when the pH of the culture rises above 7.8. The organism is highly aerobic and had doubling times as low as ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1248-1254.1976

    authors: Okon Y,Albrecht SL,Burris RH

    更新日期:1976-09-01 00:00:00

  • Phospholipid biosynthesis in some anaerobic bacteria.

    abstract::We have identified and characterized enzymes of phospholipid synthesis in two plasmalogen-rich anaerobes. Megasphaera elsdenii and Veillonella parvula, and one anaerobe lacking plasmalogens. Desulfovibrio vulgaris. All three species contained phosphatidate cytidylyltransferase and phosphatidylserine synthase. Phosphat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.1.57-61.1981

    authors: Silber P,Borie RP,Mikowski EJ,Goldfine H

    更新日期:1981-07-01 00:00:00

  • Enzymatic characterization and in vivo function of five terminal oxidases in Pseudomonas aeruginosa.

    abstract::The ubiquitous opportunistic pathogen Pseudomonas aeruginosa has five aerobic terminal oxidases: bo(3)-type quinol oxidase (Cyo), cyanide-insensitive oxidase (CIO), aa3-type cytochrome c oxidase (aa3), and two cbb(3)-type cytochrome c oxidases (cbb(3)-1and cbb(3)-2). These terminal oxidases are differentially regulate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02176-14

    authors: Arai H,Kawakami T,Osamura T,Hirai T,Sakai Y,Ishii M

    更新日期:2014-12-01 00:00:00

  • Characterization and construction of molecular cloning vehicles within Staphylococcus aureus.

    abstract::Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.402-413.1978

    authors: Wilson CR,Baldwin NJ

    更新日期:1978-10-01 00:00:00

  • Metabolic control and autogenous regulation of nit-3, the nitrate reductase structural gene of Neurospora crassa.

    abstract::In Neurospora crassa, the expression of nit-3, the structural gene which encodes nitrate reductase, is highly regulated and requires both nitrate induction and nitrogen catabolite derepression. The major nitrogen regulatory gene, nit-2, acts in a positive fashion to turn on the expression of nit-3 and other nitrogen-r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.657-661.1988

    authors: Fu YH,Marzluf GA

    更新日期:1988-02-01 00:00:00

  • Transcription factor GreA contributes to resolving promoter-proximal pausing of RNA polymerase in Bacillus subtilis cells.

    abstract::Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00086-11

    authors: Kusuya Y,Kurokawa K,Ishikawa S,Ogasawara N,Oshima T

    更新日期:2011-06-01 00:00:00

  • Deciphering morphological determinants of the helix-shaped Leptospira.

    abstract::Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. Defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05695-11

    authors: Slamti L,de Pedro MA,Guichet E,Picardeau M

    更新日期:2011-11-01 00:00:00

  • The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.

    abstract::In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.21.6772-6778.1999

    authors: De Wulf P,Kwon O,Lin EC

    更新日期:1999-11-01 00:00:00

  • Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes.

    abstract::The DNA-binding factor BifA (previously called VF1) binds upstream of the developmentally regulated site-specific recombinase gene xisA in the cyanobacterium Anabaena sp. strain PCC 7120. Besides binding xisA, BifA also binds the glnA, rbcL, and nifH promoter regions. DNase I footprint analysis of BifA binding to glnA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1214-1223.1994

    authors: Ramasubramanian TS,Wei TF,Golden JW

    更新日期:1994-03-01 00:00:00

  • Evolutionary analysis of Burkholderia pseudomallei identifies putative novel virulence genes, including a microbial regulator of host cell autophagy.

    abstract::Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00718-13

    authors: Singh AP,Lai SC,Nandi T,Chua HH,Ooi WF,Ong C,Boyce JD,Adler B,Devenish RJ,Tan P

    更新日期:2013-12-01 00:00:00