Abstract:
:Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of unknown function. Putative transcriptional terminators flank this locus, raising the possibility that these genes are expressed as a single transcriptional unit. However, S1 nuclease protection and primer extension experiments identified probable transcriptional start sites upstream of the p32, p21, p50, and rpsD genes. Each was preceded at the appropriate spacing by the -10-like sequence TTAAAATT, but the -35 regions were not conserved. Analysis of the M. pneumoniae genome sequence indicated that this promoter-like sequence is found upstream of only a limited number of open reading frames, including the genes for P65 and P200, which are structurally related to HMW1 and HMW3. Promoter deletion studies demonstrated that the promoter-like region upstream of p21 was necessary for the expression of p30 and an hmw3-cat fusion in M. pneumoniae, while deletion of the promoter-like region upstream of p32 had no apparent effect. Analysis by reverse transcription-PCR confirmed transcriptional linkage of all the open reading frames in the hmw gene cluster. Taken together, these findings suggest that the genes of this locus constitute an operon expressed from overlapping transcripts.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Waldo RH 3rd,Popham PL,Romero-Arroyo CE,Mothershed EA,Lee KK,Krause DCdoi
10.1128/JB.181.16.4978-4985.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
4978-85issue
16eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.628-633.1966
更新日期:1966-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.181.21.6689-6696.1999
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更新日期:1969-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.150.1.141-147.1982
更新日期:1982-04-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1981-12-01 00:00:00
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更新日期:2005-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.130.1.232-241.1977
更新日期:1977-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.180.24.6773-6775.1998
更新日期:1998-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1971-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.1.399-402.1982
更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.174.8.2724-2728.1992
更新日期:1992-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.185.6.2031-2035.2003
更新日期:2003-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.94.4.1225-1235.1967
更新日期:1967-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1961-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3854-3859.1996
更新日期:1996-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00154-10
更新日期:2010-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5322-5324.1989
更新日期:1989-10-01 00:00:00