Abstract:
:It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the C-terminal cytoplasmic domain were analyzed. The cytoplasmic domain of the ExoP protein was shown to have ATPase activity. Mutations in the highly conserved Walker A ATP-binding motif prevented ATPase activity of the ExoP protein. Phenotypically, these mutations resulted in much lower levels of succinoglycan which consisted only of monomers of the octasaccharide repeating unit. The ExoP protein has similarities to proteins with autophosphorylating protein tyrosine kinase activity. We found that ExoP was phosphorylated on tyrosine and that site-directed mutagenesis of specific tyrosine residues in the cytoplasmic domain of ExoP resulted in an altered ratio of low-molecular-weight succinoglycan to high-molecular-weight succinoglycan.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Niemeyer D,Becker Adoi
10.1128/jb.183.17.5163-5170.2001keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
5163-70issue
17eissn
0021-9193issn
1098-5530journal_volume
183pub_type
杂志文章abstract::When titanium(III) citrate was used as electron donor for the reduction of methyl coenzyme M by the methyl coenzyme M methylreductase system of Methanobacterium thermoautotrophicum delta H, component A1 was no longer required. The simpler system thus obtained required components A2, A3, and C as well as catalytic amou...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3946-3952.1988
更新日期:1988-09-01 00:00:00
abstract::The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.4.1002-1007.1998
更新日期:1998-02-01 00:00:00
abstract::In Salmonella enterica serovar Typhimurium, the stationary-phase sigma factor sigma(S) (RpoS) is required for virulence, stress resistance, biofilm formation, and development of the rdar morphotype. This morphotype is a multicellular behavior characterized by expression of the adhesive extracellular matrix components ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00033-06
更新日期:2006-06-01 00:00:00
abstract::Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.151-157.1964
更新日期:1964-07-01 00:00:00
abstract::A second cluster of genes encoding the E1 alpha, E1 beta, and E2 subunits of branched-chain alpha-keto acid dehydrogenase (BCDH), bkdFGH, has been cloned and characterized from Streptomyces avermitilis, the soil microorganism which produces anthelmintic avermectins. Open reading frame 1 (ORF1) (bkdF, encoding E1 alpha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.12.3504-3511.1995
更新日期:1995-06-01 00:00:00
abstract::The level of extracellular alpha-amylase (EC 3.2.1.1) of Bacillus subtilis Marburg was increased about fivefold by introducing the amyR marker from B. natto 1212 through transformation. amyR2 of B. natto 1212 has been assumed to determine a high level of alpha-amylase of the organism. The gene acts specifically on alp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.410-415.1974
更新日期:1974-08-01 00:00:00
abstract::A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.2.500-508.1981
更新日期:1981-08-01 00:00:00
abstract::Spendlove, Rex S. (California State Department of Public Health, Berkeley), Edwin H. Lennette, Charles O. Knight, and Jean N. Chin. Production in FL cells of infectious and potentially infectious reovirus. J. Bacteriol. 92:1036-1040. 1966.-A comparative study was made of the development in, and release from, FL cells ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.4.1036-1040.1966
更新日期:1966-10-01 00:00:00
abstract::The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.14.4328-4337.1994
更新日期:1994-07-01 00:00:00
abstract::Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3915-3923.1988
更新日期:1988-09-01 00:00:00
abstract::Binding sites for the Escherichia coli protein integration host factor (IHF) include a set of conserved bases that can be summarized by the consensus sequence WATCAANNNNTTR (W is dA or dT, R is dA or dG, and N is any nucleotide). However, additional 5'-proximal bases, whose common feature is a high dA+dT content, are ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.10.2999-3006.1994
更新日期:1994-05-01 00:00:00
abstract::The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the bi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00196-12
更新日期:2012-07-01 00:00:00
abstract::Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.12.3031-3038.1998
更新日期:1998-06-01 00:00:00
abstract::RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis. Increased translation of rpoS mRNA during growth at low temperature, after osmotic challenge, or with a constitutiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00206-06
更新日期:2006-07-01 00:00:00
abstract::A beta-1,4-xylan hydrolase (xylanase A) produced by Erwinia chrysanthemi D1 isolated from corn was analyzed with respect to its secondary structure and enzymatic function. The pH and temperature optima for the enzyme were found to be pH 6.0 and 35 degrees C, with a secondary structure under those conditions that consi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.6.2093-2100.2001
更新日期:2001-03-01 00:00:00
abstract::A low level of recombination between homologous regions of plasmids and the host chromosome occurred during cell growth. The plasmids contained antibiotic resistance markers on the homologous regions which were only expressed when they were integrated into the chromosome. Such recombination took place in Rec+ rec-2 mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.458-459.1986
更新日期:1986-10-01 00:00:00
abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00274-15
更新日期:2015-08-01 00:00:00
abstract::The lytD1 mutant of Escherichia coli exhibits temperature-sensitive growth which is attributed to cellular autolysis at the restrictive temperature. Either of two cloned phage lambda genes, identified as cI and cro, suppressed the lytD1(Ts) lysis phenotype, suggesting that lytD encodes a DNA-binding protein with a DNA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.893-895.1991
更新日期:1991-01-01 00:00:00
abstract::Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.975-982.1975
更新日期:1975-03-01 00:00:00
abstract::The antibiotic d-cycloserine is an effective inhibitor of alanine racemase. The lack of inhibition by l-cycloserine of alanine racemase from Staphylococcus aureus led Roze and Strominger to formulate the cycloserine hypothesis. This hypothesis states that d-cycloserine has the conformation required of the substrates o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.978-987.1972
更新日期:1972-06-01 00:00:00
abstract::Berg, Gerald (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Eugene K. Harris, Shih L. Chang, and Kenneth A. Busch. Quantitation of viruses by the plaque technique. J. Bacteriol. 85:691-700. 1963.-This paper presents the results of a study on overcrowding as it occurred with several strains of enterovi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.691-700.1963
更新日期:1963-03-01 00:00:00
abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01415-09
更新日期:2010-03-01 00:00:00
abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.6.1989-1993.1967
更新日期:1967-12-01 00:00:00
abstract::The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.668-677.1977
更新日期:1977-02-01 00:00:00
abstract::Studies on the crisp-1 (cr-1), cyclic adenosine 3',5'-monophosphate (cAMP)-deficient mutants of Neurospora crassa were undertaken to characterize the response of these mutants to exogenous cyclic nucleotides and cyclic nucleotide analogs. A growth tube bioassay and a radioimmune assay for cyclic nucleotides yielded th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1140-1144.1979
更新日期:1979-03-01 00:00:00
abstract::The respiratory metabolism of Schizosaccharomyces pombe 972h(-), a fission, haplontic, "petite negative" yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions. l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth. d-Lactat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.473-481.1970
更新日期:1970-10-01 00:00:00
abstract::Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4691-4699.1994
更新日期:1994-08-01 00:00:00
abstract::We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4643-4645.1997
更新日期:1997-07-01 00:00:00
abstract::Toxin-antitoxin (TA) systems have been reported in the genomes of most bacterial species, and their role when located on the chromosome is still debated. TA systems are particularly abundant in the massive cassette arrays associated with chromosomal superintegrons (SI). Here, we describe the characterization of two su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01389-12
更新日期:2013-05-01 00:00:00
abstract::Magnetotactic bacteria synthesize specific organelles, the magnetosomes, which are membrane-enveloped crystals of the magnetic mineral magnetite (Fe(3)O(4)). The biomineralization of magnetite involves the uptake and intracellular accumulation of large amounts of iron. However, it is not clear how iron uptake and biom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00319-10
更新日期:2010-08-01 00:00:00