A second branched-chain alpha-keto acid dehydrogenase gene cluster (bkdFGH) from Streptomyces avermitilis: its relationship to avermectin biosynthesis and the construction of a bkdF mutant suitable for the production of novel antiparasitic avermectins.

abstract：:Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations ...

journal_title：Journal of bacteriology

authors： Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

更新日期：2009-12-01 00:00:00

abstract：:In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed con...

journal_title：Journal of bacteriology

authors： Tani A,Ishige T,Sakai Y,Kato N

更新日期：2001-03-01 00:00:00

abstract：:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modu...

journal_title：Journal of bacteriology

authors： Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammon...

journal_title：Journal of bacteriology

authors： REPASKE R

更新日期：1962-02-01 00:00:00

abstract：:The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl-beta-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtai...

journal_title：Journal of bacteriology

authors： Azuma I,Yamamura Y,Tanaka Y,Kosaka K,Mori T

更新日期：1973-01-01 00:00:00

abstract：:We report a phenomenon similar to catabolite repression in Rhizobium meliloti. Succinate, which allows the highest observed rate of growth of R. meliloti, caused an immediate reduction of beta-galactosidase activity when added to cells growing in lactose. A Lac- mutant was unaltered in nodulation and nitrogen fixation...

journal_title：Journal of bacteriology

authors： Ucker DS,Signer ER

更新日期：1978-12-01 00:00:00

abstract：:The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...

journal_title：Journal of bacteriology

authors： Caberoy NB,Welch RD,Jakobsen JS,Slater SC,Garza AG

更新日期：2003-10-01 00:00:00

abstract：:A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

journal_title：Journal of bacteriology

authors： Bonsen PP,Spudich JA,Nelson DL,Kornberg A

更新日期：1969-04-01 00:00:00

abstract：:Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...

journal_title：Journal of bacteriology

authors： Rifkind D,Palmer JD

更新日期：1966-10-01 00:00:00

abstract：:Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of un...

journal_title：Journal of bacteriology

authors： Waldo RH 3rd,Popham PL,Romero-Arroyo CE,Mothershed EA,Lee KK,Krause DC

更新日期：1999-08-01 00:00:00

abstract：:In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...

journal_title：Journal of bacteriology

authors： Ballesta JP,De Garcia CL,Schaechter M

更新日期：1973-10-01 00:00:00

abstract：:Phosphate is an essential nutrient that Staphylococcus aureus and other pathogens must acquire from the host during infection. While inorganic monophosphate (Pi) is the preferred source of this nutrient, bacteria can also obtain it from phosphate-containing organic molecules. The Pi-responsive regulator PhoPR is neces...

journal_title：Journal of bacteriology

authors： Kelliher JL,Leder Macek AJ,Grudzinski KM,Radin JN,Kehl-Fie TE

更新日期：2020-10-22 00:00:00

abstract：:ibeA is a virulence factor found in some extraintestinal pathogenic Escherichia coli (ExPEC) strains from the B2 phylogenetic group and particularly in newborn meningitic and avian pathogenic strains. It was shown to be involved in the invasion process of the newborn meningitic strain RS218. In a previous work, we sho...

journal_title：Journal of bacteriology

authors： Fléchard M,Cortes MA,Répérant M,Germon P

更新日期：2012-09-01 00:00:00

abstract：:The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...

journal_title：Journal of bacteriology

authors： Lantz MS,Switalski LM,Kornman KS,Höök M

更新日期：1985-08-01 00:00:00

abstract：:The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

journal_title：Journal of bacteriology

authors： Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

更新日期：2010-03-01 00:00:00

abstract：:Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...

journal_title：Journal of bacteriology

authors： Marketon MM,González JE

更新日期：2002-07-01 00:00:00

abstract：:The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resu...

journal_title：Journal of bacteriology

authors： Mortimer MW,McDermott TR,York GM,Walker GC,Kahn ML

更新日期：1999-12-01 00:00:00

abstract：:After heating at 65 C, ribosomes isolated from Bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from Escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 C. The stability of ribosomes was also determined by measurement...

journal_title：Journal of bacteriology

authors： Friedman SM,Axel R,Weinstein IB

更新日期：1967-05-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. An effective live attenuated vaccine would be extremely useful in the prevention of R. equi disease in horses. Toward that end, we have developed an efficient transposon mu...

journal_title：Journal of bacteriology

authors： Ashour J,Hondalus MK

更新日期：2003-04-01 00:00:00

abstract：:MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We al...

journal_title：Journal of bacteriology

authors： Chubiz LM,Rao CV

更新日期：2010-09-01 00:00:00

abstract：:Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

journal_title：Journal of bacteriology

authors： Brambl R

更新日期：1975-06-01 00:00:00

abstract：:In contrast to Escherichia coli and Salmonella typhimurium, Bacillus subtilis could convert ethionine to S-adenosylethionine (SAE), as can Saccharomyces cerevisiae. This conversion was essential for growth inhibition by ethionine because metE mutants which were deficient in S-adenosylmethionine synthetase activity, we...

journal_title：Journal of bacteriology

authors： Allen ER,Orrego C,Wabiko H,Freese E

更新日期：1986-04-01 00:00:00

abstract：:We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

journal_title：Journal of bacteriology

authors： Condee CW,Summers AO

更新日期：1992-12-01 00:00:00

abstract：:The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...

journal_title：Journal of bacteriology

authors： Bylund GO,Lövgren JM,Wikström PM

更新日期：2001-10-01 00:00:00

abstract：:The spoIIG promoter is used by RNA polymerase containing sigma A (E sigma A), the primary form of RNA polymerase found in vegetative cells in Bacillus subtilis. However, the spoIIG promoter is active only after the onset of sporulation. Activation of the spoIIG promoter requires the product of the spo0A gene (Spo0A). ...

journal_title：Journal of bacteriology

authors： Satola SW,Baldus JM,Moran CP Jr

更新日期：1992-03-01 00:00:00

abstract：:Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...

journal_title：Journal of bacteriology

authors： Linn T,Goman M,Scaife J

更新日期：1979-11-01 00:00:00

abstract：:Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts ...

journal_title：Journal of bacteriology

authors： Conley MP,Berg HC

更新日期：1984-06-01 00:00:00

abstract：:Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone ...

journal_title：Journal of bacteriology

authors： Teague WM,Henney HR Jr

更新日期：1973-11-01 00:00:00

abstract：:Synthesis of formate dehydrogenase coupled to formate hydrogenlyase activity in Escherichia coli was found to require the product of the fhlA gene. Transcription of fdhF, the gene coding for the 80-kilodalton (kDa) selenopeptide of formate dehydrogenase, was not detected in an fhlA genetic background. Mutations in the...

journal_title：Journal of bacteriology

authors： Sankar P,Lee JH,Shanmugam KT

更新日期：1988-12-01 00:00:00

abstract：:To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. cit...

journal_title：Journal of bacteriology

authors： Duret S,Danet JL,Garnier M,Renaudin J

更新日期：1999-12-01 00:00:00