Global mutational analysis of NtrC-like activators in Myxococcus xanthus: identifying activator mutants defective for motility and fruiting body development.

abstract：:A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...

journal_title：Journal of bacteriology

authors： Bardowski J,Ehrlich SD,Chopin A

更新日期：1994-09-01 00:00:00

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:The magnetosomes of many magnetotactic bacteria consist of membrane-enveloped magnetite crystals, whose synthesis is favored by a low redox potential. However, the cellular redox processes governing the biomineralization of the mixed-valence iron oxide have remained unknown. Here, we show that in the alphaproteobacter...

journal_title：Journal of bacteriology

authors： Li Y,Katzmann E,Borg S,Schüler D

更新日期：2012-09-01 00:00:00

abstract：:The human pathogen Vibrio cholerae carries a chromosomal superintegron (SI). The SI contains an array of hundreds of gene cassettes organized in tandem which are stable under conditions when no particular stress is applied to bacteria (such as during laboratory growth). Rearrangements of these cassettes are catalyzed ...

journal_title：Journal of bacteriology

authors： Baharoglu Z,Krin E,Mazel D

更新日期：2012-04-01 00:00:00

abstract：:L. Sibold, M. Henriquet, O. Possot, and J.-P. Aubert (Res. Microbiol. 142:5-12, 1991) cloned and sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences of the nifH ORFs from M. barkeri showed that nifH1 clusters with nifH gene...

journal_title：Journal of bacteriology

authors： Chien YT,Zinder SH

更新日期：1994-11-01 00:00:00

abstract：:Smith, P. B. (Communicable Disease Center, Atlanta, Ga.). Growth of staphylococci on mercuric chloride agar. J. Bacteriol. 84:1016-1019. 1962.-An attempt has been made to corroborate a report that "epidemic" strains of staphylococci are more resistant to mercuric salts than are "nonepidemic" strains. A comparison of m...

journal_title：Journal of bacteriology

authors： SMITH PB

更新日期：1962-11-01 00:00:00

abstract：:In this report, we describe some of the characteristics of the Comamonas testosteroni B-356 biphenyl (BPH)-chlorobiphenyl dioxygenase system, which includes the terminal oxygenase, an iron-sulfur protein (ISPBPH) made up of an alpha subunit (51 kDa) and a beta subunit (22 kDa) encoded by bphA and bphE, respectively; a...

journal_title：Journal of bacteriology

authors： Hurtubise Y,Barriault D,Powlowski J,Sylvestre M

更新日期：1995-11-01 00:00:00

abstract：:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

journal_title：Journal of bacteriology

authors： Shum AC,Markovetz AJ

更新日期：1974-06-01 00:00:00

abstract：:Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...

journal_title：Journal of bacteriology

authors： Chang HC,Mulkins GJ,Dyer JC,Sorger GJ

更新日期：1975-08-01 00:00:00

abstract：:Peripheral hyphae were separated from the remaining thallus of Rhizoctonia solani in exponential and stationary phases of growth. The QO(2) in whole cells of peripheral hyphae from young fungal colonies was on the average 2.6 times and the protein content 1.6 times greater than in peripheral hyphae from old fungal col...

journal_title：Journal of bacteriology

authors： Skowronski BS,Gottlieb D

更新日期：1970-11-01 00:00:00

abstract：:In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...

journal_title：Journal of bacteriology

authors： Holmes M,Rickert M,Pierucci O

更新日期：1980-04-01 00:00:00

abstract：:The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...

journal_title：Journal of bacteriology

authors： Okai M,Ohtsuka J,Imai LF,Mase T,Moriuchi R,Tsuda M,Nagata K,Nagata Y,Tanokura M

更新日期：2013-06-01 00:00:00

abstract：:DNA helicase II, the product of the uvrD gene, has been implicated in DNA repair, replication, and recombination. Because the phenotypes of individual uvrD alleles vary significantly, we constructed deletion-insertion mutations in the uvrD gene to determine the phenotype of cells lacking DNA helicase II. Deletion muta...

journal_title：Journal of bacteriology

authors： Washburn BK,Kushner SR

更新日期：1991-04-01 00:00:00

abstract：:The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

journal_title：Journal of bacteriology

authors： Chou CC,Lin TW,Chen CY,Wang AH

更新日期：2003-07-01 00:00:00

abstract：:Escherichia coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first few days after plating at densities above 10(8) on plates lacking the required amino acid. They lose viability for some days, and then a subpopulation recovers and there is cell turnover. At very low plating d...

journal_title：Journal of bacteriology

authors： Bridges BA,Ereira S

更新日期：1998-06-01 00:00:00

abstract：:Toxin-antitoxin (TA) systems have been reported in the genomes of most bacterial species, and their role when located on the chromosome is still debated. TA systems are particularly abundant in the massive cassette arrays associated with chromosomal superintegrons (SI). Here, we describe the characterization of two su...

journal_title：Journal of bacteriology

authors： Guérout AM,Iqbal N,Mine N,Ducos-Galand M,Van Melderen L,Mazel D

更新日期：2013-05-01 00:00:00

abstract：:The segregation of mating loci with other unselected genes was analyzed in recombinants obtained from matings of Nocardia canicruria and N. erythropolis. The loci C/c and E/e control nocardial compatibility. Four mating genotype combinations were observed: cE, Ce, CE, and ce. Strains of N. erythropolis bear the genoty...

journal_title：Journal of bacteriology

authors： Brownell GH,Kelly KL

更新日期：1969-07-01 00:00:00

abstract：:We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...

journal_title：Journal of bacteriology

authors： Nathanson NM,Schleif R

更新日期：1973-08-01 00:00:00

abstract：:Mutants of Bacillis subtilis that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a 32P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the rsbU gene, which encodes a phosphatase that indirectly activates SigB, the...

journal_title：Journal of bacteriology

authors： Rothstein DM,Lazinski D,Osburne MS,Sonenshein AL

更新日期：2017-06-27 00:00:00

abstract：:The mobilizable Bacteroides element NBU2 (11 kbp) was found originally in two Bacteroides clinical isolates, Bacteroides fragilis ERL and B. thetaiotaomicron DOT. At first, NBU2 appeared to be very similar to another mobilizable Bacteroides element, NBU1, in a 2.5-kbp internal region, but further examination of the fu...

journal_title：Journal of bacteriology

authors： Wang J,Shoemaker NB,Wang GR,Salyers AA

更新日期：2000-06-01 00:00:00

abstract：:Two methods of mutagenesis, chemical alkylation and insertion of the tetracycline resistance transposon Tn10, were used to generate mutants of Salmonella typhimurium which had reduced levels of endodeoxyribonucleolytic activity. The chemically induced mutations defined a locus, endA, which was cotransduced with serA a...

journal_title：Journal of bacteriology

authors： Zabel DJ,Trucksis M,Depew RE

更新日期：1980-10-01 00:00:00

abstract：:Escherichia coli ssrA encodes a small stable RNA molecule, tmRNA, that has many diverse functions, including tagging abnormal proteins for degradation, supporting phage growth, and modulating the activity of DNA binding proteins. Here we show that ssrA plays a role in Salmonella enterica serovar Typhimurium pathogenes...

journal_title：Journal of bacteriology

authors： Julio SM,Heithoff DM,Mahan MJ

更新日期：2000-03-01 00:00:00

abstract：:A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

journal_title：Journal of bacteriology

authors： Kim C,Lorenz WW,Hoopes JT,Dean JF

更新日期：2001-08-01 00:00:00

abstract：:Polysomes were extracted from Bacillus subtilis cells starved for a required amino acid. The monosome peak appeared soon after starvation; no difference in the rate of degradation was detected when the cells were starved for arginine or tryptophan in a double auxotroph. RNA production during starvation was not inhibit...

journal_title：Journal of bacteriology

authors： Barlati S

更新日期：1970-03-01 00:00:00

abstract：:Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...

journal_title：Journal of bacteriology

authors： Wang J,Hofnung M,Charbit A

更新日期：2000-01-01 00:00:00

abstract：:Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro except at an acidic pH. M. tuberculosis is uniquely susceptible to PZA, but other mycobacteria as well as nonmycobacteria...

journal_title：Journal of bacteriology

authors： Zhang Y,Scorpio A,Nikaido H,Sun Z

更新日期：1999-04-01 00:00:00

abstract：:The products of the bvgAS locus coordinately regulate expression of the Bordetella pertussis virulence regulon in response to environmental signals. Transcription of bvgAS-activated genes is nearly eliminated by several modulating conditions, including the presence of sulfate anion or nicotinic acid and growth at low ...

journal_title：Journal of bacteriology

authors： Miller JF,Johnson SA,Black WJ,Beattie DT,Mekalanos JJ,Falkow S

更新日期：1992-02-01 00:00:00

abstract：:Outer membrane preparations of Bdellovibrio bacteriovorus grown intraperiplasmically on Escherichia coli containing OmpF were prepared by the Triton X-100 procedure of Schnaitman (J. Bacteriol. 108:545-552, 1971). They contained a protein that migrated to almost the same position as E. coli OmpF in sodium dodecyl sulf...

journal_title：Journal of bacteriology

authors： Rayner JR,Cover WH,Martinez RJ,Rittenberg SC

更新日期：1985-08-01 00:00:00

abstract：:Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...

journal_title：Journal of bacteriology

authors： Inselburg J

更新日期：1974-08-01 00:00:00

abstract：:Bacterial spores have long been recognized as the sturdiest known life forms on earth, revealing extraordinary resistance to a broad range of environmental assaults. A family of highly conserved spore-specific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP), plays a major role in...

journal_title：Journal of bacteriology

authors： Frenkiel-Krispin D,Sack R,Englander J,Shimoni E,Eisenstein M,Bullitt E,Horowitz-Scherer R,Hayes CS,Setlow P,Minsky A,Wolf SG

更新日期：2004-06-01 00:00:00