Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1.

Abstract:

:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modular designs, M. leprae PBP1 is related to Escherichia coli PBP1a and PBP1b, bienzymatic proteins with transglycosylase and transpeptidase activities. When produced in E. coli, His tag-labelled derivatives of M. leprae PBP1 adopt the correct membrane topology, with the bulk of the polypeptide chain on the surface of the plasma membrane. They defy attempts at solubilization with all the detergents tested except cetyltrimethylammonium bromide. The solubilized PBP1 derivatives can be purified by affinity chromatography on Ni2+-nitrilotriacetic acid agarose. They have low affinities for the usual penicillins and cephalosporins.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM

doi

10.1128/jb.178.6.1707-1711.1996

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

1707-11

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.

    abstract::The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2742-2748.1988

    authors: Miller P,Mueller J,Hill K,Taber H

    更新日期:1988-06-01 00:00:00

  • Genetic analysis of thymidine-resistant and low-thymine-requiring mutants of Escherichia coli K-12 induced by bacteriophage Mu-1.

    abstract::Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.475-484.1975

    authors: Buxton RS

    更新日期:1975-02-01 00:00:00

  • Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

    abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.816-822.1979

    authors: Brandriss MC

    更新日期:1979-06-01 00:00:00

  • Fine structure of Vibrio cholerae during toxin production.

    abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1393-1401.1969

    authors: Kennedy JR,Richardson SH

    更新日期:1969-12-01 00:00:00

  • Coupling of NAD+ biosynthesis and nicotinamide ribosyl transport: characterization of NadR ribonucleotide kinase mutants of Haemophilus influenzae.

    abstract::Previously, we characterized a pathway necessary for the processing of NAD+ and for uptake of nicotinamide riboside (NR) in Haemophilus influenzae. Here we report on the role of NadR, which is essential for NAD+ utilization in this organism. Different NadR variants with a deleted ribonucleotide kinase domain or with a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.13.4410-4420.2005

    authors: Merdanovic M,Sauer E,Reidl J

    更新日期:2005-07-01 00:00:00

  • A topological model for the general aromatic amino acid permease, AroP, of Escherichia coli.

    abstract::The general aromatic amino acid permease, AroP, of Escherichia coli is responsible for the active transport of phenylalanine, tyrosine, and tryptophan. A proposed topological model for the AroP permease, consisting of 12 hydrophobic transmembrane spans connected by hydrophilic loops, is very similar to that of the clo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.10.3317-3323.1997

    authors: Cosgriff AJ,Pittard AJ

    更新日期:1997-05-01 00:00:00

  • Solitary BioY proteins mediate biotin transport into recombinant Escherichia coli.

    abstract::Energy-coupling factor (ECF) transporters form a large group of vitamin uptake systems in prokaryotes. They are composed of highly diverse, substrate-specific, transmembrane proteins (S units), a ubiquitous transmembrane protein (T unit), and homo- or hetero-oligomeric ABC ATPases. Biotin transporters represent a spec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00350-13

    authors: Finkenwirth F,Kirsch F,Eitinger T

    更新日期:2013-09-01 00:00:00

  • Thermal death of a hydrocarbon bacterium in a nonaqueous fluid.

    abstract::A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.3.1287-1292.1973

    authors: Severance MM,LaRock PA

    更新日期:1973-12-01 00:00:00

  • Asymmetric expression of the gyrase B gene from the replication-competent chromosome in the Caulobacter crescentus predivisional cell.

    abstract::The bacterium Caulobacter crescentus undergoes an asymmetric cell division resulting in the formation of two different daughter cells, a motile swarmer cell and a nonmotile stalked cell. These two cell types differ in their program of gene expression, their ability to replicate DNA, and the physical properties of thei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.21.6970-6981.1993

    authors: Rizzo MF,Shapiro L,Gober J

    更新日期:1993-11-01 00:00:00

  • Cyclic AMP-induced conformational change of cyclic AMP receptor protein (CRP): intragenic suppressors of cyclic AMP-independent CRP mutations.

    abstract::We isolated and characterized crp mutations in Escherichia coli that allow cyclic AMP (cAMP) receptor protein to function without cAMP. These mutants defined a region involved in the cAMP-induced allosteric change of cAMP receptor protein that is necessary for activation of the protein. Currently, we have isolated int...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1417-1422.1988

    authors: Garges S,Adhya S

    更新日期:1988-04-01 00:00:00

  • A genetic analysis of Xis and FIS interactions with their binding sites in bacteriophage lambda.

    abstract::The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.19.5954-5963.1991

    authors: Numrych TE,Gumport RI,Gardner JF

    更新日期:1991-10-01 00:00:00

  • Genetic dissection of T4 lysis.

    abstract::t is the holin gene for coliphage T4, encoding a 218-amino-acid (aa) protein essential for the inner membrane hole formation that initiates lysis and terminates the phage infection cycle. T is predicted to be an integral membrane protein that adopts an N(in)-C(out) topology with a single transmembrane domain (TMD). Th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01548-14

    authors: Moussa SH,Lawler JL,Young R

    更新日期:2014-06-01 00:00:00

  • Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells.

    abstract::THREE PREVIOUSLY STUDIED R FACTORS WERE USED: 222/R4, controlling transmissible resistance to sulfonamide, streptomycin, chloromycetin, and tetracycline (SU(r) SM(r) CM(r) TC(r)); 222/R3, a derivative of 222/R4 (now termed 222/R3W) having lost TC(r); and R15, controlling infectious resistance to SU and SM only. Two ty...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.1.166-177.1970

    authors: Nisioka T,Mitani M,Clowes RC

    更新日期:1970-07-01 00:00:00

  • Nucleotide and derived amino acid sequences of the major porin of Comamonas acidovorans and comparison of porin primary structures.

    abstract::The DNA sequence of the gene which codes for the major outer membrane porin (Omp32) of Comamonas acidovorans has been determined. The structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. Comparisons with amino acid sequences of outer membrane protei...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.7.2196-2205.1991

    authors: Gerbl-Rieger S,Peters J,Kellermann J,Lottspeich F,Baumeister W

    更新日期:1991-04-01 00:00:00

  • Uranyl nitrate inhibition of transport systems in Saccharomyces cerevisiae.

    abstract::Uranyl nitrate inhibited the transport of several amino acids, a vitamin, and a disaccharide into yeast. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.1205-1206.1971

    authors: Maxwell WA,Metzler R,Spoerl E

    更新日期:1971-03-01 00:00:00

  • ABC Transporters Required for Hexose Uptake by Clostridium phytofermentans.

    abstract::The mechanisms by which bacteria uptake solutes across the cell membrane broadly impact their cellular energetics. Here, we use functional genomic, genetic, and biophysical approaches to reveal how Clostridium (Lachnoclostridium) phytofermentans, a model bacterium that ferments lignocellulosic biomass, uptakes plant h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00241-19

    authors: Cerisy T,Iglesias A,Rostain W,Boutard M,Pelle C,Perret A,Salanoubat M,Fierobe HP,Tolonen AC

    更新日期:2019-07-10 00:00:00

  • Transfer and incorporation of genes controlling beta-D-galactosidase synthesis from Hfr and F' donors of Escherichia coli.

    abstract::Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling beta-d-galactosidase synthesis from Hfr and F' donors of Escherichia coli. J. Bacteriol. 92:1378-1382. 1966.-Comparisons were made between Hfr(1) and F(13) donors with respect t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.5.1378-1382.1966

    authors: Ganesan AK,Rotman B

    更新日期:1966-11-01 00:00:00

  • Shuttle plasmids for Escherichia coli and Clostridium perfringens.

    abstract::Small plasmids which replicate in both Escherichia coli and Clostridium perfringens were made by recombining E. coli plasmid pBR322 with three different small (less than 4 kilobases) plasmids native to C. perfringens. Subsequently, two homologous, though distinct, tetracycline resistance determinants (tet) from other ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.465-471.1984

    authors: Squires CH,Heefner DL,Evans RJ,Kopp BJ,Yarus MJ

    更新日期:1984-08-01 00:00:00

  • The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster.

    abstract::The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.17.4930-4932.2002

    authors: Gál J,Szvetnik A,Schnell R,Kálmán M

    更新日期:2002-09-01 00:00:00

  • The Rickettsia prowazekii ExoU homologue possesses phospholipase A1 (PLA1), PLA2, and lyso-PLA2 activities and can function in the absence of any eukaryotic cofactors in vitro.

    abstract::Here we have characterized the Rickettsia prowazekii RP534 protein, a homologue of the Pseudomonas aeruginosa ExoU phospholipase A (PLA) secreted cytotoxin. Our studies showed that purified recombinant RP534 PLA possessed the predicted PLA(2) and lyso-PLA(2) activities based on what has been published for P. aeruginos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00141-11

    authors: Housley NA,Winkler HH,Audia JP

    更新日期:2011-09-01 00:00:00

  • Genome sequence of Rhizobium grahamii CCGE502, a broad-host-range symbiont with low nodulation competitiveness in Phaseolus vulgaris.

    abstract::Here we present the genome sequence of Rhizobium grahamii CCGE502. R. grahamii groups with other newly described broad-host-range species, which are not very efficient Phaseolus vulgaris symbionts, with a wide geographic distribution and which constitutes a novel Rhizobium clade. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01785-12

    authors: Althabegoiti MJ,Lozano L,Torres-Tejerizo G,Ormeño-Orrillo E,Rogel MA,González V,Martínez-Romero E

    更新日期:2012-12-01 00:00:00

  • Functional interaction of the tonA/tonB receptor system in Escherichia coli.

    abstract::Host range mutants of phage T1 (T1h), which productively infected tonB mutants of Escherichia coli, were isolated. The phage mutants were inactivated by isolated outer membranes of E. coli in contrast to the wild-type phage, which only adsorbed reversibly. For the infection process, the tonB function is apparently onl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.1.190-197.1978

    authors: Hantke K,Braun V

    更新日期:1978-07-01 00:00:00

  • Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii.

    abstract::Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00779-17

    authors: Gelsinger DR,DiRuggiero J

    更新日期:2018-04-09 00:00:00

  • FpvIR control of fpvA ferric pyoverdine receptor gene expression in Pseudomonas aeruginosa: demonstration of an interaction between FpvI and FpvR and identification of mutations in each compromising this interaction.

    abstract::FpvR is a presumed cytoplasmic membrane-associated anti-sigma factor that controls the activities of extracytoplasmic function sigma factors PvdS and FpvI responsible for transcription of pyoverdine biosynthetic genes and the ferric pyoverdine receptor gene, fpvA, respectively. Using deletion analysis and an in vivo b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5648-5657.2005

    authors: Rédly GA,Poole K

    更新日期:2005-08-01 00:00:00

  • Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis.

    abstract::The spoIIG promoter is used by RNA polymerase containing sigma A (E sigma A), the primary form of RNA polymerase found in vegetative cells in Bacillus subtilis. However, the spoIIG promoter is active only after the onset of sporulation. Activation of the spoIIG promoter requires the product of the spo0A gene (Spo0A). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.5.1448-1453.1992

    authors: Satola SW,Baldus JM,Moran CP Jr

    更新日期:1992-03-01 00:00:00

  • Functional complementation between bacterial MDR-like export systems: colicin V, alpha-hemolysin, and Erwinia protease.

    abstract::The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7549-7556.1991

    authors: Fath MJ,Skvirsky RC,Kolter R

    更新日期:1991-12-01 00:00:00

  • A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis.

    abstract::Lactococcus lactis, a gram-positive organism widely used in the food industry, is a potential candidate for the secretion of biologically useful proteins. We examined the secretion efficiency and capacity of L. lactis by using the Staphylococcus aureus nuclease (Nuc) as a heterologous model protein. When expressed in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1895-1903.1998

    authors: Le Loir Y,Gruss A,Ehrlich SD,Langella P

    更新日期:1998-04-01 00:00:00

  • DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

    abstract::Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.5.1266-1276.1964

    authors: KONDO E,MITSUHASHI S

    更新日期:1964-11-01 00:00:00

  • Amino acid uptake and energy coupling dependent on photosynthesis in Anacystis nidulans.

    abstract::The photoautotrophic cyanobacterium Anacystis nidulans was used to investigate the membrane transport of branched-chain, neutral amino acids and its dependence on photosynthetic reactions. The uptake of alpha-amino [1-14C]isobutyric acid and L-[1-14C]leucine followed Michaelis, Menten kinetics and resulted in an energ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.1.229-236.1982

    authors: Lee-Kaden J,Simonis W

    更新日期:1982-07-01 00:00:00

  • Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract::The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6672-6676.1994

    authors: Kim SW,Kim CY,Benisek WF,Choi KY

    更新日期:1994-11-01 00:00:00