Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1.


:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modular designs, M. leprae PBP1 is related to Escherichia coli PBP1a and PBP1b, bienzymatic proteins with transglycosylase and transpeptidase activities. When produced in E. coli, His tag-labelled derivatives of M. leprae PBP1 adopt the correct membrane topology, with the bulk of the polypeptide chain on the surface of the plasma membrane. They defy attempts at solubilization with all the detergents tested except cetyltrimethylammonium bromide. The solubilized PBP1 derivatives can be purified by affinity chromatography on Ni2+-nitrilotriacetic acid agarose. They have low affinities for the usual penicillins and cephalosporins.


J Bacteriol


Journal of bacteriology


Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM




Has Abstract


1996-03-01 00:00:00












  • Transduction with integration-defective mutants of Salmonella typhimurium bacteriophage KB1.

    abstract::Phage KB1 has gained use as a generalized transducing phage for Salmonella typhimurium, including strains resistant to phage P22. Integration-defective mutants of KB1 have now been isolated; one of these, int-1, is recommended for transduction when nonlysogenic recombinants are desired. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McIntire SA

    更新日期:1974-02-01 00:00:00

  • Rhizobium (Sinorhizobium) meliloti phn genes: characterization and identification of their protein products.

    abstract::In Escherichia coli, the phn operon encodes proteins responsible for the uptake and breakdown of phosphonates. The C-P (carbon-phosphorus) lyase enzyme encoded by this operon which catalyzes the cleavage of C-P bonds in phosphonates has been recalcitrant to biochemical characterization. To advance the understanding of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Parker GF,Higgins TP,Hawkes T,Robson RL

    更新日期:1999-01-01 00:00:00

  • Convenient method for detecting 14CO2 in multiple samples: application to rapid screening for mutants.

    abstract::A procedure is presented for the rapid screening of bacterial colonies to detect mutants unable to produce 14CO2 from a labeled precursor. The method is especially useful for mass screening for mutants that cannot be easily detected by their phenotypic characteristics. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tabor H,Tabor CW,Hafner EW

    更新日期:1976-10-01 00:00:00

  • Effects of environmental changes on expression of the oligopeptide permease (opp) genes of Borrelia burgdorferi.

    abstract::We analyzed expression of a putative oligopeptide permease (Opp) of Borrelia burgdorferi. Unlike the opp operons of other bacteria for which there is a single substrate binding protein, B. burgdorferi codes for three substrate binding proteins (OppA-I to -III) in its opp operon and an additional two homologs on plasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Wang XG,Lin B,Kidder JM,Telford S,Hu LT

    更新日期:2002-11-01 00:00:00

  • Outer membrane of Pseudomonas aeruginosa: heat- 2-mercaptoethanol-modifiable proteins.

    abstract::A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hancock RE,Carey AM

    更新日期:1979-12-01 00:00:00

  • Polymer-Producing Species of Arthrobacter.

    abstract::Gasdorf, Helen J. (Northern Regional Research Laboratory, Peoria, Ill.), R. G. Benedict, M. C. Cadmus, R. F. Anderson, and R. W. Jackson. Polymer-producing species of Arthrobacter. J. Bacteriol. 90:147-150.1965.-Two slime-producing microorganisms, designated as NRRL B-1973 and NRRL B-1797, were isolated from a Guatema...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gasdorf HJ,Benedict RG,Cadmus MC,Anderson RF,Jackson RW

    更新日期:1965-07-01 00:00:00

  • Ultrastructure of the Bacteroides nodosus cell envelope layers and surface.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Every D,Skerman TM

    更新日期:1980-02-01 00:00:00

  • Proline transport and metabolism in Rickettsia prowazekii.

    abstract::Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Winkler HH,Daugherty RM

    更新日期:1984-05-01 00:00:00

  • Activation of ribulose 1,5-bisphosphate carboxylase from Rhodopseudomonas sphaeroides: probable role of the small subunit.

    abstract::The activation properties of the form I and form II ribulose 1,5-bisphosphate carboxylases from Rhodopseudomonas sphaeroides were examined. Both enzymes have a requirement of Mg2+ for optimal activity. Mn2+, Ni2+, and Co2+ can also support activity of the form I enzyme, whereas only Mn2+ can substitute for Mg2+ with t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gibson JL,Tabita FR

    更新日期:1979-12-01 00:00:00

  • Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.

    abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gilden RV,Carp RI

    更新日期:1966-03-01 00:00:00

  • rec-2-dependent phage recombination in Haemophilus influenzae.

    abstract::The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kupfer DM,McCarthy D

    更新日期:1992-08-01 00:00:00

  • Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli.

    abstract::Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJ

    更新日期:2002-03-01 00:00:00

  • Complete genome sequences of three Pseudomonas aeruginosa isolates with phenotypes of polymyxin B adaptation and inducible resistance.

    abstract::Clinical "superbug" isolates of Pseudomonas aeruginosa were previously observed to be resistant to several antibiotics, including polymyxin B, and/or to have a distinct, reproducible adaptive polymyxin resistance phenotype, identified by observing "skipped" wells (appearance of extra turbid wells) during broth microdi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Boyle B,Fernandez L,Laroche J,Kukavica-Ibrulj I,Mendes CM,Hancock RW,Levesque RC

    更新日期:2012-01-01 00:00:00

  • Deoxyribonucleic acid relatedness among species of Erwinia and between Erwinia species and other enterobacteria.

    abstract::Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brenner DJ,Fanning GR,Steigerwalt AG

    更新日期:1972-04-01 00:00:00


    abstract::Freeman, B. M. (Houghton Poultry Research Station, Huntingdon, England), and L. G. Chubb. Effect of Salmonella gallinarum on certain Krebs cycle intermediates of domestic fowl. J. Bacteriol. 88:93-95. 1964.-In chickens infected with Salmonella gallinarum, the citric acid content of the blood rose but that of the liver...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: FREEMAN BM,CHUBB LG

    更新日期:1964-07-01 00:00:00

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    abstract::Mycelial mats of Ascodesmis sphaerospora were fixed and embedded for electron microscopy, and thin sections of 1-mm blocks, taken from the 1st to the 7th mm behind the hyphal tips, were cut parallel to the long axis of the hyphae. The hyphal tip region is characterized by an outer zone of electron-transparent vesicles...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brenner DM,Carroll GC

    更新日期:1968-02-01 00:00:00

  • VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system.

    abstract::The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

    更新日期:2008-07-01 00:00:00

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    abstract::The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Krieg NR,Tomelty JP,Wells JS Jr

    更新日期:1967-11-01 00:00:00

  • Analysis of autolysins in temperature-sensitive morphological mutants of Bacillus subtilis.

    abstract::The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Brown WC,Wilson CR,Lukehart S,Young FE,Shiflett MA

    更新日期:1976-01-01 00:00:00

  • Regulation of glutamine synthetase. VI. Interactions of inhibitors for Bacillus licheniformis glutamine synthetase.

    abstract::The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hubbard JS,Stadtman ER

    更新日期:1967-10-01 00:00:00

  • Cell division of cycle of Bacillus subtilis: evidence of variability in period D.

    abstract::In Bacillus subtilis the deoxyribonucleic acid content and the extent of cell division during inhibition of chromosome replication increased as a function of the average cell mass, independent of the growth rate. At each growth rate, mass, deoxyribonucleic acid, and residual division varied in different cultures. The ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Holmes M,Rickert M,Pierucci O

    更新日期:1980-04-01 00:00:00

  • Chromosomal and extrachromosomal synthesis of exfoliative toxin from Staphylococcus hyicus.

    abstract::Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sato H,Watanabe T,Higuchi K,Teruya K,Ohtake A,Murata Y,Saito H,Aizawa C,Danbara H,Maehara N

    更新日期:2000-07-01 00:00:00

  • 16S ribosomal DNA amplification for phylogenetic study.

    abstract::A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Weisburg WG,Barns SM,Pelletier DA,Lane DJ

    更新日期:1991-01-01 00:00:00

  • Observations on replication and cell division in synchronous cultures of the blue-green alga Anacystis nidulans.

    abstract::A second synchrony induction treatment, half-way through the deoxyribonucleic acid replication process of an already synchronized culture, resulted in defective subsequent cell growth. This supports a previous explanation for the partial loss of viability after synchrony induction. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Herdman M,Carr NG

    更新日期:1971-08-01 00:00:00

  • VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa.

    abstract::Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor closely related plasmids of about 70 kb that are essential for virulence. At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a decrease in growth rate and the release of large amounts of proteins called Yops. Here we describe ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Allaoui A,Scheen R,Lambert de Rouvroit C,Cornelis GR

    更新日期:1995-08-01 00:00:00

  • Fluorescein amine media for rapid differentiation of staphylococci.

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    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: KIMLER A

    更新日期:1961-07-01 00:00:00

  • Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant.

    abstract::The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sciochetti SA,Blakely GW,Piggot PJ

    更新日期:2001-05-01 00:00:00

  • NosR, a membrane-bound regulatory component necessary for expression of nitrous oxide reductase in denitrifying Pseudomonas stutzeri.

    abstract::The regulatory element NosR was identified within the nos region of the denitrification gene cluster of Pseudomonas stutzeri ZoBell (ATCC 14405) and characterized. It is essential for expression of the N2O reductase encoded by nosZ immediately downstream of nosR. The nosR region was initially identified by Tn5 mutagen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Cuypers H,Viebrock-Sambale A,Zumft WG

    更新日期:1992-08-01 00:00:00

  • Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

    abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Stitt BL,Mosig G

    更新日期:1989-07-01 00:00:00

  • Quantification of group A colicin import sites.

    abstract::Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Duché D,Letellier L,Géli V,Bénédetti H,Baty D

    更新日期:1995-09-01 00:00:00