Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1.


:Cosmid B577, a member of the collection of ordered clones corresponding to the genome of Mycobacterium leprae, contains a gene, provisionally called pon1, that encodes an 821-amino-acid-residue high-molecular-mass class A penicillin-binding protein, provisionally called PBP1. With similar amino acid sequences and modular designs, M. leprae PBP1 is related to Escherichia coli PBP1a and PBP1b, bienzymatic proteins with transglycosylase and transpeptidase activities. When produced in E. coli, His tag-labelled derivatives of M. leprae PBP1 adopt the correct membrane topology, with the bulk of the polypeptide chain on the surface of the plasma membrane. They defy attempts at solubilization with all the detergents tested except cetyltrimethylammonium bromide. The solubilized PBP1 derivatives can be purified by affinity chromatography on Ni2+-nitrilotriacetic acid agarose. They have low affinities for the usual penicillins and cephalosporins.


J Bacteriol


Journal of bacteriology


Basu J,Mahapatra S,Kundu M,Mukhopadhyay S,Nguyen-Distèche M,Dubois P,Joris B,Van Beeumen J,Cole ST,Chakrabarti P,Ghuysen JM




Has Abstract


1996-03-01 00:00:00












  • Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli.

    abstract::The PhoQ/PhoP two-component system is repressed by divalent cations, such as Mg(2+) and Ca(2+), in the growth medium and stimulated by low pH and certain cationic antimicrobial peptides. In Escherichia coli, it was recently shown that the histidine kinase PhoQ is also modulated by at least two additional factors, the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lippa AM,Goulian M

    更新日期:2012-03-01 00:00:00

  • The A1A0 ATPase from Methanosarcina mazei: cloning of the 5' end of the aha operon encoding the membrane domain and expression of the proteolipid in a membrane-bound form in Escherichia coli.

    abstract::Three additional ATPase genes, clustered in the order ahaH, ahaI, and ahaK, were found upstream of the previously characterized genes ahaECFABDG coding for the archaeal A1A0 ATPase from Methanosarcina mazei. ahaH, the first gene in the cluster, is preceded by a conserved promoter sequence. Northern blot analysis revea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ruppert C,Wimmers S,Lemker T,Müller V

    更新日期:1998-07-01 00:00:00

  • Impact of either elevated or decreased levels of cytochrome bd expression on Shigella flexneri virulence.

    abstract::Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Way SS,Sallustio S,Magliozzo RS,Goldberg MB

    更新日期:1999-02-01 00:00:00

  • Isolation and characterization of a mutant of Escherichia coli blocked in the synthesis of putrescine.

    abstract::A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hirshfield IN,Rosenfeld HJ,Leifer Z,Maas WK

    更新日期:1970-03-01 00:00:00

  • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.

    abstract::Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gomez A,Ladiré M,Marcille F,Fons M

    更新日期:2002-01-01 00:00:00

  • A new pathway for salvaging the coenzyme B12 precursor cobinamide in archaea requires cobinamide-phosphate synthase (CbiB) enzyme activity.

    abstract::The ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobU in Salmonella enterica). The latter activity is required for cobinamide salvaging in bacteria. This paper reports evidence that ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Woodson JD,Zayas CL,Escalante-Semerena JC

    更新日期:2003-12-01 00:00:00

  • Effect of environmental pH on morphological development of Candida albicans is mediated via the PacC-related transcription factor encoded by PRR2.

    abstract::The ability to respond to ambient pH is critical to the growth and virulence of the fungal pathogen Candida albicans. This response entails the differential expression of several genes affecting morphogenesis. To investigate the mechanism of pH-dependent gene expression, the C. albicans homolog of pacC, designated PRR...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ramon AM,Porta A,Fonzi WA

    更新日期:1999-12-01 00:00:00

  • Thermolability of Malic Dehydrogenase from the Obligate Psychrophile Vibrio marinus.

    abstract::Langridge, Patricia (Oregon State University, Corvallis), and Richard Y. Morita. Thermolability of malic dehydrogenase from the obligate psychrophile Vibrio marinus. J. Bacteriol. 92:418-423. 1966.-The thermolability of malic dehydrogenase in whole cells of Vibrio marinus MP-1 grown at 15 C was compared with that of c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Langridge P,Morita RY

    更新日期:1966-08-01 00:00:00

  • Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12.

    abstract::The aim of this study was to identify the compatible solutes accumulated by Pseudomonas putida S12 subjected to osmotic stress. In response to reduced water activity, P. putida S12 accumulated Nalpha-acetylglutaminylglutamine amide (NAGGN) simultaneously with a novel compatible solute identified as mannitol (using 13C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kets EP,Galinski EA,de Wit M,de Bont JA,Heipieper HJ

    更新日期:1996-12-01 00:00:00

  • Control of chromosome replication in thymine-requiring strains of Bacillus subtilis 168.

    abstract::Study of the replication pattern of a number of B. subtilis 168 strains under controlled physiological conditions revealed great interstrain variation in control of replication. Replication patterns were calculated from ratios of purA16/leu-8 and purA16/metB5 transformation frequency. The thymine-independent strains a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gillin FD,Ganesan AT

    更新日期:1975-09-01 00:00:00

  • Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: cheD mutations affect the structure and function of the Tsr transducer.

    abstract::The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Callahan AM,Parkinson JS

    更新日期:1985-01-01 00:00:00

  • Coupling of asymmetric division to polar placement of replication origin regions in Bacillus subtilis.

    abstract::Entry into sporulation in Bacillus subtilis is characterized by the formation of a polar septum, which asymmetrically divides the developing cell into forespore (the smaller cell) and mother cell compartments, and by migration of replication origin regions to extreme opposite poles of the cell. Here we show that polar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Graumann PL,Losick R

    更新日期:2001-07-01 00:00:00

  • EutR is a direct regulator of genes that contribute to metabolism and virulence in enterohemorrhagic Escherichia coli O157:H7.

    abstract::Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. EHEC encodes the ethanolamine utilization (eut) operon that allows EHEC to metabolize EA and gain a competitive advan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Luzader DH,Clark DE,Gonyar LA,Kendall MM

    更新日期:2013-11-01 00:00:00

  • Analysis of hemF gene function and expression in Rhodobacter sphaeroides 2.4.1.

    abstract::The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Zeilstra-Ryalls JH,Schornberg KL

    更新日期:2006-01-01 00:00:00

  • Contribution of σ70 and σN Factors to Expression of Class II pilE in Neisseria meningitidis.

    abstract::Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lobanovska M,Tang CM,Exley RM

    更新日期:2019-09-20 00:00:00

  • Rhodobacter capsulatus OlsA is a bifunctional enzyme active in both ornithine lipid and phosphatidic acid biosynthesis.

    abstract::The Rhodobacter capsulatus genome contains three genes (olsA [plsC138], plsC316, and plsC3498) that are annotated as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate) acyltransferase (AGPAT). Of these genes, olsA was previously shown to be an O-acyltransferase in the second step of ornithine lipid biosynthesis, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Aygun-Sunar S,Bilaloglu R,Goldfine H,Daldal F

    更新日期:2007-12-01 00:00:00

  • Suppressed nonsense mutations in the araC gene of Escherichia coli provide three novel variant proteins.

    abstract::A total of 400 suppressible mutations have been isolated in the araC gene of Escherichia coli. Based on deletion mapping, growth patterns when suppressed, and intragenic recombination, 37 mutants have been determined to contain unique mutations. Rapid plate assays were developed to test for each of the three AraC prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schecter JB

    更新日期:1983-06-01 00:00:00

  • Regulation of acetate kinase isozymes and its importance for mixed-acid fermentation in Lactococcus lactis.

    abstract::Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Puri P,Goel A,Bochynska A,Poolman B

    更新日期:2014-04-01 00:00:00

  • Identification of methylation sites in Thermotoga maritima chemotaxis receptors.

    abstract::Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Perez E,Zheng H,Stock AM

    更新日期:2006-06-01 00:00:00


    abstract::Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章



    更新日期:1965-06-01 00:00:00

  • Spy1, a histidine-containing phosphotransfer signaling protein, regulates the fission yeast cell cycle through the Mcs4 response regulator.

    abstract::Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Aoyama K,Mitsubayashi Y,Aiba H,Mizuno T

    更新日期:2000-09-01 00:00:00

  • In situ analysis of sulfur species in sulfur globules produced from thiosulfate by Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes.

    abstract::The Firmicutes Thermoanaerobacter sulfurigignens and Thermoanaerobacterium thermosulfurigenes convert thiosulfate, forming sulfur globules inside and outside cells. X-ray absorption near-edge structure analysis revealed that the sulfur consisted mainly of sulfur chains with organic end groups similar to sulfur formed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lee YJ,Prange A,Lichtenberg H,Rohde M,Dashti M,Wiegel J

    更新日期:2007-10-01 00:00:00

  • Characterization of Mycoplasma pulmonis variants isolated from rabbits. I. Identification and properties of isolates.

    abstract::Mycoplasma showing at least two colony types were isolated from the nares and oropharynx of New Zealand white rabbits. Two strains were purified by single-colony passages and characterized. Morphology by phase-contrast and electron microscopy was typical of Mycoplasmataceae. Both grew anaerobically as well as aerobica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Deeb BJ,Kenny GE

    更新日期:1967-04-01 00:00:00

  • Genome sequence of the Spinosyns-producing bacterium Saccharopolyspora spinosa NRRL 18395.

    abstract::Saccharopolyspora spinosa is a Gram-positive bacterium that produces spinosad, a well-known biodegradable insecticide that is used for agricultural pest control and has an excellent environmental and mammalian toxicological profile. Here, we present the first draft genome sequence of the type strain Saccharopolyspora ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pan Y,Yang X,Li J,Zhang R,Hu Y,Zhou Y,Wang J,Zhu B

    更新日期:2011-06-01 00:00:00

  • Cloning of the gene for myxobacterial hemagglutinin and isolation and analysis of structural gene mutations.

    abstract::Myxobacterial hemagglutinin (MBHA) is a major developmentally induced protein that accumulates during the period of cellular aggregation in the bacterium Myxococcus xanthus. It has been shown that this lectin is targeted to the cell surface and periplasmic space of developmental cells, suggesting that it may play a ro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Romeo JM,Zusman DR

    更新日期:1987-08-01 00:00:00

  • Genome sequence of Lactococcus garvieae 8831, isolated from rainbow trout lactococcosis outbreaks in Spain.

    abstract::Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Aguado-Urda M,López-Campos GH,Gibello A,Cutuli MT,López-Alonso V,Fernández-Garayzábal JF,Blanco MM

    更新日期:2011-08-01 00:00:00

  • A Bacillus subtilis gene encoding a protein similar to nucleotide sugar transferases influences cell shape and viability.

    abstract::Bacillus subtilis gene ypfP, which is located at 196 degrees on the genetic map, shows similarity to both the monogalactosyldiacylglycerol synthase gene of Cucumis sativus, which encodes a galactosyltransferase, and the murG genes of B. subtilis, Escherichia coli, Haemophilus influenzae, and Synechocystis sp. strain P...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Price KD,Roels S,Losick R

    更新日期:1997-08-01 00:00:00

  • Polyribosomes from Bacillus subtilis during amino acid starvation in the presence and in the absence of actinomycin.

    abstract::Polysomes were extracted from Bacillus subtilis cells starved for a required amino acid. The monosome peak appeared soon after starvation; no difference in the rate of degradation was detected when the cells were starved for arginine or tryptophan in a double auxotroph. RNA production during starvation was not inhibit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Barlati S

    更新日期:1970-03-01 00:00:00

  • Conditions affecting transformation of a group H streptococcus.

    abstract::Schlissel, Harvey J. (The University of Kansas, Lawrence), and C. P. Sword. Conditions affecting transformation of a group H streptococcus. J. Bacteriol. 92:1357-1363. 1966.-A defined transforming medium (DTM) containing buffer and 5 to 10 mug per ml of deoxyribonucleic acid was developed to study the physical and che...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Schlissel HJ,Sword CP

    更新日期:1966-11-01 00:00:00

  • Isolation and characterization of an Escherichia coli seryl-tRNA synthetase mutant with a large increase in Km for serine.

    abstract::A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, resulting in the substitution of the residue alanine 262 by valine in motif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Willison JC,Härtlein M,Leberman R

    更新日期:1995-06-01 00:00:00