Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic di-GMP levels, and enhanced dispersal.

Abstract:

:Bacteria in biofilms often undergo active dispersal events and revert to a free-swimming, planktonic state to complete the biofilm life cycle. The signaling molecule nitric oxide (NO) was previously found to trigger biofilm dispersal in the opportunistic pathogen Pseudomonas aeruginosa at low, nontoxic concentrations (N. Barraud, D. J. Hassett, S. H. Hwang, S. A. Rice, S. Kjelleberg, and J. S. Webb, J. Bacteriol. 188:7344-7353, 2006). NO was further shown to increase cell motility and susceptibility to antimicrobials. Recently, numerous studies revealed that increased degradation of the secondary messenger cyclic di-GMP (c-di-GMP) by specific phosphodiesterases (PDEs) triggers a planktonic mode of growth in eubacteria. In this study, the potential link between NO and c-di-GMP signaling was investigated by performing (i) PDE inhibitor studies, (ii) enzymatic assays to measure PDE activity, and (iii) direct quantification of intracellular c-di-GMP levels. The results suggest a role for c-di-GMP signaling in triggering the biofilm dispersal event induced by NO, as dispersal requires PDE activity and addition of NO stimulates PDE and induces the concomitant decrease in intracellular c-di-GMP levels in P. aeruginosa. Furthermore, gene expression studies indicated global responses to low, nontoxic levels of NO in P. aeruginosa biofilms, including upregulation of genes involved in motility and energy metabolism and downregulation of adhesins and virulence factors. Finally, site-directed mutagenesis of candidate genes and physiological characterization of the corresponding mutant strains uncovered that the chemotaxis transducer BdlA is involved in the biofilm dispersal response induced by NO.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Barraud N,Schleheck D,Klebensberger J,Webb JS,Hassett DJ,Rice SA,Kjelleberg S

doi

10.1128/JB.00975-09

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

7333-42

issue

23

eissn

0021-9193

issn

1098-5530

pii

JB.00975-09

journal_volume

191

pub_type

杂志文章
  • Spermidine requirement for Bacillus thuringiensis ribosomes in cell-free phenylalanine incorporation.

    abstract::A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolute...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.3.734-740.1970

    authors: Changchien LM,Aronson JN

    更新日期:1970-09-01 00:00:00

  • Efficient plasmid transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts.

    abstract::A procedure for efficient transformation of Streptomyces ambofaciens and Streptomyces fradiae protoplasts with plasmid DNA was developed. Transformation frequencies with S. fradiae protoplasts were strongly influenced by the temperatures for cell growth, protoplast formation, and protoplast regeneration. Transformatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.1.180-185.1985

    authors: Matsushima P,Baltz RH

    更新日期:1985-07-01 00:00:00

  • Temporal global changes in gene expression during temperature transition in Yersinia pestis.

    abstract::DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37 degrees C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6298-6305.2004

    authors: Motin VL,Georgescu AM,Fitch JP,Gu PP,Nelson DO,Mabery SL,Garnham JB,Sokhansanj BA,Ott LL,Coleman MA,Elliott JM,Kegelmeyer LM,Wyrobek AJ,Slezak TR,Brubaker RR,Garcia E

    更新日期:2004-09-01 00:00:00

  • DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.

    abstract::The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.2148-2150.2001

    authors: Ishigo-Oka D,Ogasawara N,Moriya S

    更新日期:2001-03-01 00:00:00

  • Evidence from Mutational Analysis for a Single Transmembrane Substrate Binding Site in the Histidine ATP-Binding Cassette Transporter of Salmonella enterica Serovar Typhimurium.

    abstract::The histidine ATP-binding cassette (ABC) transporter of Salmonella enterica serovar Typhimurium is among the best-studied type I ABC import systems. The transporter consists of two transmembrane subunits, HisQ and HisM, and a homodimer of the nucleotide-binding subunit, HisP. Substrates are delivered by two periplasmi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00521-18

    authors: Heuveling J,Landmesser H,Schneider E

    更新日期:2018-12-20 00:00:00

  • Effects of homocitrate, homocitrate lactone, and fluorohomocitrate on nitrogenase in NifV- mutants of Azotobacter vinelandii.

    abstract::Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5403-5405.1991

    authors: Madden MS,Paustian TD,Ludden PW,Shah VK

    更新日期:1991-09-01 00:00:00

  • DRS is far less divergent than streptococcal inhibitor of complement of group A streptococcus.

    abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01619-06

    authors: Sagar V,Kumar R,Ganguly NK,Menon T,Chakraborti A

    更新日期:2007-04-01 00:00:00

  • Conceptual Model of Biofilm Antibiotic Tolerance That Integrates Phenomena of Diffusion, Metabolism, Gene Expression, and Physiology.

    abstract::Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00307-19

    authors: Stewart PS,White B,Boegli L,Hamerly T,Williamson KS,Franklin MJ,Bothner B,James GA,Fisher S,Vital-Lopez FG,Wallqvist A

    更新日期:2019-10-21 00:00:00

  • Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.

    abstract::The mycoplasma Acholeplasma laidlawii was shown to have mechanisms for both host cell and ultraviolet (UV) reactivation of UV-irradiated mycoplasmaviruses. Host cell reactivation was examined by comparing the survival abilities of UV-irradiated double-stranded deoxyribonucleic acid mycoplasmavirus plated on both untre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1424-1427.1977

    authors: Das J,Nowak JA,Maniloff J

    更新日期:1977-03-01 00:00:00

  • Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence.

    abstract::The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00506-06

    authors: Petrosino JF,Xiang Q,Karpathy SE,Jiang H,Yerrapragada S,Liu Y,Gioia J,Hemphill L,Gonzalez A,Raghavan TM,Uzman A,Fox GE,Highlander S,Reichard M,Morton RJ,Clinkenbeard KD,Weinstock GM

    更新日期:2006-10-01 00:00:00

  • Mutations at several loci cause increased expression of ribonucleotide reductase in Escherichia coli.

    abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05989-11

    authors: Feeney MA,Ke N,Beckwith J

    更新日期:2012-03-01 00:00:00

  • Electron microscope studies of conditional spore cortexless mutants of Bacillus sphaericus.

    abstract::Spore cortex of conditional cortexless mutants of Bacillus sphaericus 9602 was not detectable by electron microscopy unless the medium was supplemented with meso-alpha,epsilon-diaminopimelic acid during sporulation. Other spore structures appeared normal. Spore shape was quite irregular in the absence of meso-alpha,ep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1568-1570.1976

    authors: Imae Y,Strominger MB,Strominger JL

    更新日期:1976-09-01 00:00:00

  • Isolation and characterization of vanadate-resistant mutants of Saccharomyces cerevisiae.

    abstract::Cellular vanadium metabolism was studied in Saccharomyces cerevisiae by isolating and characterizing vanadate [VO4(3-), V(V)]-resistant mutants. Vanadate growth inhibition was reversed by the removal of the vanadate from the medium, and vanadate resistance was found to be a recessive trait. Vanadate-resistant mutants ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.611-617.1985

    authors: Willsky GR,Leung JO,Offermann PV Jr,Plotnick EK,Dosch SF

    更新日期:1985-11-01 00:00:00

  • Genetic Basis Underlying the Hyperhemolytic Phenotype of Streptococcus agalactiae Strain CNCTC10/84.

    abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00504-20

    authors: Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

    更新日期:2020-11-04 00:00:00

  • Nucleotide sequence analysis and expression of the minimum REPI replication region and incompatibility determinants of pColV-K30.

    abstract::We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.2195-2201.1989

    authors: Perez-Casal JF,Gammie AE,Crosa JH

    更新日期:1989-04-01 00:00:00

  • Conditional lethality of deletions which include uvrB in strains of Escherichia coli lacking deoxyribonucleic acid polymerase I.

    abstract::Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.676-681.1972

    authors: Shizuya H,Dykhuizen D

    更新日期:1972-11-01 00:00:00

  • Deletion between direct repeats in T7 DNA stimulated by double-strand breaks.

    abstract::An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.19.5904-5911.1994

    authors: Kong D,Masker W

    更新日期:1994-10-01 00:00:00

  • The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence.

    abstract::Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.12.3031-3038.1998

    authors: Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJ

    更新日期:1998-06-01 00:00:00

  • Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus.

    abstract::Guanosine tetraphosphate (ppGpp) is a key mediator of stringent control, an adaptive response of bacteria to amino acid starvation, and has thus been termed a bacterial alarmone. Previous X-ray crystallographic analysis has provided a structural basis for the transcriptional regulation of RNA polymerase activity by pp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00574-06

    authors: Kasai K,Nishizawa T,Takahashi K,Hosaka T,Aoki H,Ochi K

    更新日期:2006-10-01 00:00:00

  • New mutation (mmrA1) in Escherichia coli K-12 that affects minimal medium recovery and postreplication repair after UV irradiation.

    abstract::After UV irradiation, Escherichia coli uvrA mutant cells show higher survival on minimal than on rich growth medium, i.e., they show minimal-medium recovery. This effect of rich growth medium on survival is not observed in a uvrA mutant carrying an mmrA1 mutation, and the uvrA mmrA strain showed the same survival rate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.743-747.1983

    authors: Sharma RC,Sargentini NJ,Smith KC

    更新日期:1983-05-01 00:00:00

  • How many initiator tRNA genes does Escherichia coli need?

    abstract::Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01620-14

    authors: Samhita L,Nanjundiah V,Varshney U

    更新日期:2014-07-01 00:00:00

  • Draft genome sequence of Virgibacillus halodenitrificans 1806.

    abstract::Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01280-12

    authors: Lee SJ,Lee YJ,Jeong H,Lee SJ,Lee HS,Pan JG,Kim BC,Lee DW

    更新日期:2012-11-01 00:00:00

  • Growth and survival of Mycoplasma neurolyticum in liquid media.

    abstract::Hottle, G. A. (Naval Biological Laboratory, University of California, Berkeley), and D. N. Wright. Growth and survival of Mycoplasma neurolyticum in liquid media. J. Bacteriol. 91:1834-1839. 1966.-Maximal growth of Mycoplasma neurolyticum (between 10(8) and 10(9) colony-forming units per ml) was obtained after 3 days ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.5.1834-1839.1966

    authors: Hottle GA,Wright DN

    更新日期:1966-05-01 00:00:00

  • Analysis of the peptidoglycan structure of Bacillus subtilis endospores.

    abstract::Peptidoglycan was prepared from purified Bacillus subtilis spores of wild-type and several mutant strains. Digestion with muramidase resulted in cleavage of the glycosidic bonds adjacent to muramic acid replaced by peptide or alanine side chains but not the bonds adjacent to muramic lactam. Reduction of the resulting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.22.6451-6458.1996

    authors: Popham DL,Helin J,Costello CE,Setlow P

    更新日期:1996-11-01 00:00:00

  • Growth rate-dependent control of the rrnB P1 core promoter in Escherichia coli.

    abstract::We have extended our previous studies of the DNA sequences required for growth rate-dependent control of rRNA transcription in Escherichia coli. Utilizing a reporter system suitable for evaluation of promoters with low activities, we have found that the core promoter region of rrnB P1 (-41 to +1 with respect to the tr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.17.5560-5564.1994

    authors: Bartlett MS,Gourse RL

    更新日期:1994-09-01 00:00:00

  • Effects of the Gram stain on microspheres from thermal polyamino acids.

    abstract::Fox, Sidney W. (The Florida State University, Tallahassee) and Shuhei Yuyama. Effects of the Gram stain on microspheres from thermal polyamino acids. J. Bacteriol. 85:279-283. 1963.-Microspheres produced from acid proteinoid accept the Gram stain. The stain is negative, but microspheres produced from mixtures containi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.2.279-283.1963

    authors: FOX SW,YUYAMA S

    更新日期:1963-02-01 00:00:00

  • Crystal structure of the hyperthermophilic archaeal DNA-binding protein Sso10b2 at a resolution of 1.85 Angstroms.

    abstract::The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.14.4066-4073.2003

    authors: Chou CC,Lin TW,Chen CY,Wang AH

    更新日期:2003-07-01 00:00:00

  • Rok regulates yuaB expression during architecturally complex colony development of Bacillus subtilis 168.

    abstract::Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01170-10

    authors: Kovács AT,Kuipers OP

    更新日期:2011-02-01 00:00:00

  • Carbon monoxide metabolism of the methylotrophic acidogen Butyribacterium methylotrophicum.

    abstract::The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multip...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.1.255-263.1982

    authors: Lynd L,Kerby R,Zeikus JG

    更新日期:1982-01-01 00:00:00

  • Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci.

    abstract::The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.23.7372-7374.1994

    authors: Haft RF,Wessels MR

    更新日期:1994-12-01 00:00:00