Abstract:
:To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri, and into the S. citri oriC plasmid pBOT1, which replicates in spiroplasma cells as well as in E. coli. Electrotransformation of S. citri with the nonreplicative, recombinant plasmid pKTM1 yielded no transformants. In contrast, spiroplasmal transformants were obtained with the replicative, pBOT1-derived plasmid pCJ32. During passaging of the transformants, the plasmid was found to integrate into the chromosome by homologous recombination either at the oriC region or at the scm1 gene. In the latter case, plasmid integration by a single crossover between the scm1 gene fragment carried by the plasmid and the full-length scm1 gene carried by the chromosome led to a nonmotile phenotype. Transmission of the scm1-disrupted mutant to periwinkle (Catharanthus roseus) plants through injection into the leafhopper vector (Circulifer haematoceps) showed that the motility mutant multiplied in the insects and was efficiently transmitted to plants, in which it induced symptoms similarly to the wild-type S. citri strain. These results suggest that the spiroplasmal motility may not be essential for pathogenicity and that, more broadly, the S. citri oriC plasmids can be considered promising tools for specific gene disruption by promoting homologous recombination in S. citri, a mollicute which probably lacks a functional RecA protein.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Duret S,Danet JL,Garnier M,Renaudin Jdoi
10.1128/JB.181.24.7449-7456.1999keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
7449-56issue
24eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3466-3475.2002
更新日期:2002-07-01 00:00:00
abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.1.435-444.1975
更新日期:1975-10-01 00:00:00
abstract::Banerjee, Arun B. (University of Calcutta, Calcutta, India), and S. K. Bose. Biosynthesis of mycobacillin, a new antifungal peptide. II. Relation between streptomycin dependence and biosynthesis. J. Bacteriol. 87:1402-1405. 1964.-A streptomycin-dependent variant was isolated by a single-step mutation process from a st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.6.1402-1405.1964
更新日期:1964-06-01 00:00:00
abstract::Three evolutionarily distinct families of replicative DNA polymerases, designated polymerase B (Pol B), Pol C, and Pol D, have been identified. Members of the Pol B family are present in all three domains of life, whereas Pol C exists only in Bacteria and Pol D exists only in Archaea. Pol B enzymes replicate eukaryoti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02037-12
更新日期:2013-05-01 00:00:00
abstract::The genomic organization of Bordetella pertussis strains has been examined by using a new method. This method does not depend on the prior determination of a restriction map of the bacterial chromosome but is based on the ability to measure directly the distance between two genes. This is accomplished through the inte...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.18.5820-5826.1997
更新日期:1997-09-01 00:00:00
abstract::Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3696-3703.1989
更新日期:1989-07-01 00:00:00
abstract::Expression of the lower catabolic pathway of the TOL plasmid pWWO requires an aromatic acid inducer and the product of the xylS regulatory gene. Pseudomonas putida cells transformed with a plasmid containing the operator-promoter region of the lower pathway (OP2 [or Pm]), upstream from the catechol 2,3-dioxygenase str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3742-3746.1988
更新日期:1988-08-01 00:00:00
abstract::The growth of strain araC(c)67, which produces the enzymes of the ara operon constitutively, is inhibited by the addition of ribitol. Isolation of strains resistant to ribitol yields mutants of either the araB or araC genes. A model to account for the inhibition by ribitol is discussed. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.593-595.1970
更新日期:1970-05-01 00:00:00
abstract::In Ralstonia eutropha H16, the nitric oxide (NO)-responsive transcriptional activator NorR controls the expression of a dicistronic operon that encodes a membrane-bound NO reductase, NorB, and a protein of unknown function, NorA. The N-terminal domain (NTD) of NorR is responsible for perception of the signal molecule,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01865-06
更新日期:2007-04-01 00:00:00
abstract::In Escherichia coli, nine essential cell division proteins are known to localize to the division septum. FtsL is a 13-kDa bitopic membrane protein with a short cytoplasmic N-terminal domain, a membrane-spanning segment, and a periplasmic domain that has a repeated heptad motif characteristic of leucine zippers. Here, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.1.116-129.2000
更新日期:2000-01-01 00:00:00
abstract::Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01485-13
更新日期:2014-05-01 00:00:00
abstract::Silverman, Sidney J. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), Lynn P. Elwell, and James F. Drawdy. Influence of route of infection and other factors on growth and distribution of Listeria monocytogenes in organs of mice. J. Bacteriol. 86:355-362. 1963.-The growth of Listeria monocytogenes in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.355-362.1963
更新日期:1963-09-01 00:00:00
abstract::Cohen, Jay O. (Communicable Disease Center, Atlanta, Ga.), Glenda S. Cowart, and William B. Cherry. Antibodies against Staphylococcus aureus in nonimmunized rabbits. J. Bacteriol. 82:110-114. 1961.-Antibody against staphylococci was demonstrated in the serum of each of 36 nonimmunized specific-pathogen-free rabbits th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.1.110-114.1961
更新日期:1961-07-01 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00584-08
更新日期:2008-11-01 00:00:00
abstract::Flac mutants have been isolated in Escherichia coli K-12 which carry dominant mutations resulting in insensitivity to transfer inhibition by the Fin(+) I-like plasmid R62. These mutants were still sensitive to transfer inhibition by the fin(+) F-like plasmid R100 and, conversely, FlactraO(-) and traP(-) mutants, which...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.101-105.1974
更新日期:1974-10-01 00:00:00
abstract::ATP-dependent glucokinase is suggested to have evolved from a hypothetical polyphosphate (polyP)-dependent glucokinase (polyP-GK) via a bifunctional polyP/ATP glucokinase (polyP/ATP-GK). Here we showed that polyP-GK is present in a polyP-accumulating bacterium, Microlunatus phosphovorus. The polyP-GK produced glucose-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.18.5654-5656.2003
更新日期:2003-09-01 00:00:00
abstract::The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.3.557-561.1967
更新日期:1967-09-01 00:00:00
abstract::In a previous paper it was shown that cocultivation of Rhizobium leguminosarum with the plant Vicia sativa subsp. nigra on solid medium causes a changed mode of growth of the plant roots, resulting in thick and short roots (Tsr). The Sym plasmid present in the bacterium appeared to be essential for causing Tsr (A. A. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.2.517-522.1986
更新日期:1986-02-01 00:00:00
abstract::Disruption of gene HI0894 or HI0895 in Haemophilus influenzae Rd, homologs of Escherichia coli acrAB multidrug efflux genes, caused hypersusceptibility to erythromycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumulation of radioactive erythromycin, showing that these ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6855-6857.1997
更新日期:1997-11-01 00:00:00
abstract::Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces lividans. This pathway is used when lysine is supplied as the sole source of nitrogen for the organism. A second pathway...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.299-302.1989
更新日期:1989-01-01 00:00:00
abstract::Lockart, Royce Z., Jr. (The University of Texas, Austin). Variations in the abilities of several lines of L cells to produce and to be affected by interferon. J. Bacteriol. 89:117-122. 1965.-The amounts of interferon produced by three different lines of L cells and mouse embryonic cells when inoculated with two strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.1.117-122.1965
更新日期:1965-01-01 00:00:00
abstract::Studies with cell-free protein preparations from a series of recombinant strains of Pseudomonas denitrificans demonstrated that precorrin-3 is converted into a further trimethylated intermediate, named precorrin-3B, along the pathway to coenzyme B12. It was then shown that the part of the pathway from precorrin-3 (cal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7430-7440.1993
更新日期:1993-11-01 00:00:00
abstract:UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00820-15
更新日期:2016-07-28 00:00:00
abstract::A fragment of the Lactococcus lactis chromosome containing an open reading frame of 265 codons, denoted bglR, has been characterized. The polypeptide encoded by bglR shares 36 to 30% sequence identity with a family of regulatory proteins including ArbG from Erwinia chrysanthemi, BglG from Escherichia coli, and SacT an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5681-5685.1994
更新日期:1994-09-01 00:00:00
abstract::Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or sugg...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7196-7204.2004
更新日期:2004-11-01 00:00:00
abstract::Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01844-07
更新日期:2008-02-01 00:00:00
abstract::The regrowth of 27 temperature-sensitive division mutants of Streptococcus faecium ATCC 9790 was examined after various periods of incubation at the nonpermissive temperature. Several of the mutants blocked at various stages of septum formation or of daughter-cell separation divided in a partially or completely synchr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.427-429.1984
更新日期:1984-10-01 00:00:00
abstract::Overexpression of vsr in Escherichia coli stimulates transition and frameshift mutations. The pattern of mutations suggests that mutagenesis is due to saturation or inactivation of dam-directed mismatch repair. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.14.4294-4296.1996
更新日期:1996-07-01 00:00:00
abstract::Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23-27. 1965.-Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.1.23-27.1965
更新日期:1965-01-01 00:00:00