Selection of araB and araC mutants of Escherichia coli B-r by resistance to ribitol.

abstract：:We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of rec...

journal_title：Journal of bacteriology

authors： Pacelli LZ,Edmiston SH,Mount DW

更新日期：1979-01-01 00:00:00

abstract：:The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants r...

journal_title：Journal of bacteriology

authors： Perlman D,Twose TM,Holland MJ,Rownd RH

更新日期：1975-09-01 00:00:00

abstract：:Myxococcus xanthus, a gram-negative bacterium, has a complex life cycle. In response to starvation, most cells in a population participate in the formation of multicellular aggregates (i.e., fruiting bodies) in which cells differentiate into spores. However, some cells do not enter aggregates. In this and the two acco...

journal_title：Journal of bacteriology

authors： O'Connor KA,Zusman DR

更新日期：1991-06-01 00:00:00

abstract：:Nocardia brasiliensis possess proteolytic activities that can be readily detected in a variety of media. In a modified formulation of a growth medium originally used for Streptomyces aureofaciens, N. brasiliensis was found to secrete proteolytic enzymes, one of which was capable of hydrolyzing casein. This enzyme was ...

journal_title：Journal of bacteriology

authors： Zlotnik H,Schramm VL,Buckley HR

更新日期：1984-02-01 00:00:00

abstract：:The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...

journal_title：Journal of bacteriology

authors： Piggot PJ,Curtis CA

更新日期：1987-03-01 00:00:00

abstract：:Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

journal_title：Journal of bacteriology

authors： Stewart RC,Roth AF,Dahlquist FW

更新日期：1990-06-01 00:00:00

abstract：:The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...

journal_title：Journal of bacteriology

authors： Xu C,Liu B,Hu B,Han Y,Feng L,Allingham JS,Szarek WA,Wang L,Brockhausen I

更新日期：2011-01-01 00:00:00

abstract：:Mutations in two chromosomal genes of Escherichia coli, cpxA and cpxB, produced a temperature-sensitive growth defect that was remedied specifically by the addition of isoleucine and valine to the minimal medium. This auxotrophy was manifested only when the medium contained exogenous leucine, suggesting that mutant ce...

journal_title：Journal of bacteriology

authors： McEwen J,Silverman P

更新日期：1980-10-01 00:00:00

abstract：:Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

journal_title：Journal of bacteriology

authors： Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

更新日期：2009-04-01 00:00:00

abstract：:Analysis of the deoxyribonucleic acid prepared from Proteus mirabilis, Escherichia coli, and Serratia marcescens in an alkaline CsCl gradient has shown that there is an asymmetric distribution of guanine plus thymine residues between the complementary strands of the deoxyribonucleic acid. ...

journal_title：Journal of bacteriology

authors： Hershberger C,Mickel S,Rownd R

更新日期：1971-04-01 00:00:00

abstract：:In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

journal_title：Journal of bacteriology

authors： Oliver EJ,Mortlock RP

更新日期：1971-10-01 00:00:00

abstract：:A human isolate of Salmonella enteritidis which displayed strong pellicle formation during static broth culture and mannose-sensitive hemagglutination produced fimbriae which were morphologically indistinguishable from type 1 fimbriae of members of the family Enterobacteriaceae. Fimbrin was purified to homogeneity, an...

journal_title：Journal of bacteriology

authors： Feutrier J,Kay WW,Trust TJ

更新日期：1986-10-01 00:00:00

abstract：:A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...

journal_title：Journal of bacteriology

authors： Chen JL,Tritz GJ

更新日期：1975-01-01 00:00:00

abstract：:Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...

journal_title：Journal of bacteriology

authors： Oertel W,Goulian M

更新日期：1979-11-01 00:00:00

abstract：:The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Halofe...

journal_title：Journal of bacteriology

authors： Bochar DA,Brown JR,Doolittle WF,Klenk HP,Lam W,Schenk ME,Stauffacher CV,Rodwell VW

更新日期：1997-06-01 00:00:00

abstract：:Flavin-based electron bifurcation has recently been characterized as an essential energy conservation mechanism that is utilized by hydrogenotrophic methanogenic Archaea to generate low-potential electrons in an ATP-independent manner. Electron bifurcation likely takes place at the flavin associated with the α subunit...

journal_title：Journal of bacteriology

authors： Costa KC,Lie TJ,Xia Q,Leigh JA

更新日期：2013-11-01 00:00:00

abstract：:A previous bioinformatics-based search for small RNAs in Escherichia coli identified a novel RNA named IS183. The gene encoding this small RNA is located between and on the opposite strand of genes encoding two transcriptional regulators of the acid response, gadX (yhiX) and gadW (yhiW). Given that IS183 is encoded in...

journal_title：Journal of bacteriology

authors： Opdyke JA,Kang JG,Storz G

更新日期：2004-10-01 00:00:00

abstract：:Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

journal_title：Journal of bacteriology

authors： Hurek T,Egener T,Reinhold-Hurek B

更新日期：1997-07-01 00:00:00

abstract：:A systematic survey for the presence of plasmids in 17 different xenobiotic-degrading Sphingomonas strains was performed. In almost all analyzed strains, two to five plasmids with sizes of about 50 to 500 kb were detected by using pulsed-field gel electrophoresis. A comparison of plasmid preparations untreated or trea...

journal_title：Journal of bacteriology

authors： Basta T,Keck A,Klein J,Stolz A

更新日期：2004-06-01 00:00:00

abstract：:Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

journal_title：Journal of bacteriology

authors： Milunovic B,diCenzo GC,Morton RA,Finan TM

更新日期：2014-02-01 00:00:00

abstract：:Sphingomonas wittichii RW1 degrades chlorinated dibenzofurans and dibenzo-p-dioxins via meta cleavage. We used inverse PCR to amplify dxnB2, a gene encoding one of three meta-cleavage product (MCP) hydrolases identified in the organism that are homologues of BphD involved in biphenyl catabolism. Purified DxnB2 catalyz...

journal_title：Journal of bacteriology

authors： Seah SY,Ke J,Denis G,Horsman GP,Fortin PD,Whiting CJ,Eltis LD

更新日期：2007-06-01 00:00:00

abstract：:DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

journal_title：Journal of bacteriology

authors： Vujaklija D,Horinouchi S,Beppu T

更新日期：1993-05-01 00:00:00

abstract：:Fukuyama T. T. (University of Pennsylvania, Philadelphia) and D. J. O'Kane. Galactose metabolism. I. Pathway of carbon in fermentation by Streptococcus faecalis. J. Bacteriol. 84:793-796. 1962.-The pathway by which galactose-1-C(14) is fermented in Streptococcus faecalis was investigated using dried-cell preparations....

journal_title：Journal of bacteriology

authors： FUKUYAMA TT,O'KANE DJ

更新日期：1962-10-01 00:00:00

abstract：:A newly isolated bacterium, Pseudomonas geniculata N1, can efficiently degrade nicotine. Here we present a 4.51-Mb assembly of its genome, which is the first sequence of the P. geniculata group. The sequence contains the genes related to nicotine catabolism and may provide insights into its molecular mechanism for N-h...

journal_title：Journal of bacteriology

authors： Tang H,Yu H,Tai C,Huang K,Liu Y,Wang L,Yao Y,Wu G,Xu P

更新日期：2012-07-01 00:00:00

abstract：:The plasmid-encoded epidermin biosynthetic gene epiC of Staphylococcus epidermidis Tü3298 was expressed in Escherichia coli by using the T7 RNA polymerase-promoter system, and the gene product EpiC was identified by Western blotting (immunoblotting) with an anti-EpiC-peptide antiserum. EpiC was a hydrophobic but solub...

journal_title：Journal of bacteriology

authors： Kupke T,Gotz F

更新日期：1996-03-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide...

journal_title：Journal of bacteriology

authors： Lee CA,Grossman AD

更新日期：2007-10-01 00:00:00

abstract：:A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely me...

journal_title：Journal of bacteriology

authors： Karreman C,de Waard A

更新日期：1988-06-01 00:00:00

abstract：:Mutants of Anabaena sp. strain PCC 7120 that are incapable of sustained growth with air as the sole source of nitrogen were generated by using Tn5-derived transposons. Nitrogenase was expressed only in mutants that showed obvious morphological signs of heterocyst differentiation. Even under rigorously anaerobic condit...

journal_title：Journal of bacteriology

authors： Ernst A,Black T,Cai Y,Panoff JM,Tiwari DN,Wolk CP

更新日期：1992-10-01 00:00:00

abstract：:A number of heterologous plasmid deoxyribonucleic acids (DNAs) coding for erythromycin, tylosin, lincomycin, tetracycline, or chloramphenicol resistance have been introduced into Streptococcus pneumoniae via genetic transformation with frequencies that varied between 10(-5) to as high as 5 x 10(-1) per colony-forming ...

journal_title：Journal of bacteriology

authors： Barany F,Tomasz A

更新日期：1980-11-01 00:00:00

abstract：:Conjugative transfer and replacement of hundreds of kilobases of a bacterial chromosome can occur in vitro, but replacements in nature are either an order of magnitude smaller or involve the movement of mobile genetic elements. We discovered that two lineages of Staphylococcus aureus, including a pandemic methicillin-...

journal_title：Journal of bacteriology

authors： Robinson DA,Enright MC

更新日期：2004-02-01 00:00:00