MULTIPLE CELLULASE SYSTEM FROM STREPTOMYCES ANTIBIOTICUS.

abstract：:A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolute...

journal_title：Journal of bacteriology

authors： Changchien LM,Aronson JN

更新日期：1970-09-01 00:00:00

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Immunogenic activity of a ribosomal fraction obtained from Mycobacterium tuberculosis. J. Bacteriol. 89:1291-1298. 1965.-The highly immunogenic particulate fraction obtained from mechanically ruptured cells of the H37Ra strai...

journal_title：Journal of bacteriology

authors： YOUMANS AS,YOUMANS GP

更新日期：1965-05-01 00:00:00

abstract：:Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...

journal_title：Journal of bacteriology

authors： McCarty JS,Walker GC

更新日期：1994-02-01 00:00:00

abstract：:The PriA protein, a component of the phiX174-type primosome, was previously shown to be essential for damage-inducible DNA replication in Escherichia coli, termed inducible stable DNA replication. Here, we show that priA::kan null mutants are defective in transductional and conjugational homologous recombination and a...

journal_title：Journal of bacteriology

authors： Kogoma T,Cadwell GW,Barnard KG,Asai T

更新日期：1996-03-01 00:00:00

abstract：:Our previous work showed that pap pilin gene transcription is subject to a thermoregulatory control mechanism under which pap pilin is not transcribed at a low temperature (23 degrees C) (L. B. Blyn, B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, and D. A. Low, EMBO J. 8:613-620, 1989). In order to isolate genes i...

journal_title：Journal of bacteriology

authors： White-Ziegler CA,Low DA

更新日期：1992-11-01 00:00:00

abstract：:Both Salmonella enterica serovar Typhimurium and Escherichia coli contain the cspH gene encoding CspH, one of the cold shock proteins (CSPs). In this study, we investigated the expression of cspH in S. enterica serovar Typhimurium and found that it was induced in response to a temperature downshift during exponential ...

journal_title：Journal of bacteriology

authors： Kim BH,Bang IS,Lee SY,Hong SK,Bang SH,Lee IS,Park YK

更新日期：2001-10-01 00:00:00

abstract：:Lockart, Royce Z., Jr. (The University of Texas, Austin). Variations in the abilities of several lines of L cells to produce and to be affected by interferon. J. Bacteriol. 89:117-122. 1965.-The amounts of interferon produced by three different lines of L cells and mouse embryonic cells when inoculated with two strain...

journal_title：Journal of bacteriology

authors： LOCKART RZ Jr

更新日期：1965-01-01 00:00:00

abstract：:The host range of an octopine Ti plasmid is limited to Rhizobiaceae. This has been extended also to Escherichia coli in the form of a stable cointegrate with the wide-host-range plasmid R772. Its structure was studied by constructing a physical map of R772 and of the R772::pTiB6 cointegrate. An insertion sequence pres...

journal_title：Journal of bacteriology

authors： Hille J,van Kan J,Klasen I,Schilperoort R

更新日期：1983-05-01 00:00:00

abstract：:Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

journal_title：Journal of bacteriology

authors： Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

更新日期：2010-08-01 00:00:00

abstract：:Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...

journal_title：Journal of bacteriology

authors： MORELLO JA,DIGENIO TA,BAKER EE

更新日期：1965-06-01 00:00:00

abstract：:Random transposon Tn5 mutagenesis of Bradyrhizobium sp. (Arachis) strain NC92, a member of the cowpea cross-inoculation group, was carried out, and kanamycin-resistant transconjugants were tested for their symbiotic phenotype on three host plants: groundnut, siratro, and pigeonpea. Two nodulation (Nod- phenotype) muta...

journal_title：Journal of bacteriology

authors： Wilson KJ,Anjaiah V,Nambiar PT,Ausubel FM

更新日期：1987-05-01 00:00:00

abstract：:The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...

journal_title：Journal of bacteriology

authors： Montrone M,Eisenbach M,Oesterhelt D,Marwan W

更新日期：1998-07-01 00:00:00

abstract：:Compared with the other class B multimodular penicillin- binding proteins (PBPs), the low-affinity PBP5 responsible for penicillin resistance in Enterococcus hirae R40, has an extended non-penicillin-binding module because of the presence of an approximately 110-amino-acid E-46(-)D-160 insert downstream from the membr...

journal_title：Journal of bacteriology

authors： Mollerach ME,Partoune P,Coyette J,Ghuysen JM

更新日期：1996-03-01 00:00:00

abstract：:The bacterial plant pathogen Pseudomonas syringae depends on a type III protein secretion system and the effector proteins that it translocates into plant cells to cause disease and to elicit the defense-associated hypersensitive response on resistant plants. The availability of the P. syringae pv. tomato DC3000 genom...

journal_title：Journal of bacteriology

authors： Wehling MD,Guo M,Fu ZQ,Alfano JR

更新日期：2004-06-01 00:00:00

abstract：:SpoVM is an unusually small (26-residue-long) protein that is produced in the mother cell chamber of the sporangium during the process of sporulation in Bacillus subtilis. We investigated the subcellular localization of SpoVM, which is believed to be an amphipathic alpha-helix, by using a fusion of the sporulation pro...

journal_title：Journal of bacteriology

authors： van Ooij C,Losick R

更新日期：2003-02-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:The CpxA-CpxR two-component signal transduction pathway of Escherichia coli was studied in a mutant (pss-93) lacking phosphatidylethanolamine (PE). Several properties of this mutant are comparable to phenotypes of cpxA point mutants, indicating that this two-component pathway is activated in PE-deficient cells. In con...

journal_title：Journal of bacteriology

authors： Mileykovskaya E,Dowhan W

更新日期：1997-02-01 00:00:00

abstract：:The importance of the physiological state of a culture of Saccharomyces cerevisiae for tolerance to sudden osmotic dehydration was studied, and it was investigated whether specific osmotolerance factors were demonstrable. The microcalorimeter was used to monitor growth, and different physiological states of the cultur...

journal_title：Journal of bacteriology

authors： Blomberg A,Larsson C,Gustafsson L

更新日期：1988-10-01 00:00:00

abstract：:We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...

journal_title：Journal of bacteriology

authors： Zuberi AR,Ying CW,Parker HM,Ordal GW

更新日期：1990-12-01 00:00:00

abstract：:The catB and catC genes encode cis,cis-muconate lactonizing enzyme I (EC 5.5.1.1) and muconolactone isomerase (EC 5.3.3.4), respectively. These enzymes are required for the dissimilation of benzoate to beta-ketoadipate by Pseudomonas putida and are under coordinate transcriptional regulation. By deletion analysis and ...

journal_title：Journal of bacteriology

authors： Aldrich TL,Chakrabarty AM

更新日期：1988-03-01 00:00:00

abstract：:The RepA replication protein of plasmid pSC101 binds as a monomer to three repeated sequences (RS1, RS2, and RS3) in the replication origin of the plasmid to initiate duplication and binds as a dimer to two inversely repeated sequences (IR1 and IR2) in its promoter region (D. Manen, L. C. Upegui-Gonzalez, and L. Caro,...

journal_title：Journal of bacteriology

authors： Xia G,Manen D,Yu Y,Caro L

更新日期：1993-07-01 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:The fatty acid pattern in three hydrocarbon-utilizing bacteria during growth on various substrates was examined. The predominant fatty acids in acetate-grown cells were C(16), C(16:1), C(18:1), and Br-C(19) and the major fatty acids in propane-grown cells were C(15), C(17), C(17:1), C(18:1), and Br-C(18). When one org...

journal_title：Journal of bacteriology

authors： Dunlap KR,Perry JJ

更新日期：1967-12-01 00:00:00

abstract：:The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transpo...

journal_title：Journal of bacteriology

authors： Belitsky BR,Gustafsson MC,Sonenshein AL,Von Wachenfeldt C

更新日期：1997-09-01 00:00:00

abstract：:By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

journal_title：Journal of bacteriology

authors： Niemann H,Kwiatkowski B,Westphal U,Stirm S

更新日期：1977-04-01 00:00:00

abstract：:Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane...

journal_title：Journal of bacteriology

authors： Gregory MA,Till R,Smith MC

更新日期：2003-09-01 00:00:00

abstract：:A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

journal_title：Journal of bacteriology

authors： Bonsen PP,Spudich JA,Nelson DL,Kornberg A

更新日期：1969-04-01 00:00:00

abstract：:Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(-) vec...

journal_title：Journal of bacteriology

authors： Crasta KC,Chua KL,Subramaniam S,Frey J,Loh H,Tan HM

更新日期：2002-04-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP) is synthesized de novo in Salmonella typhimurium and is a required cofactor for many enzymes in the cell. Five kinase activities have been implicated in TPP synthesis, which involves joining a 4-methyl-5-(beta-hydroxyethyl)thiazole (THZ) moiety and a 4-amino-5-hydroxymethyl-2-methylpyrimid...

journal_title：Journal of bacteriology

authors： Petersen LA,Downs DM

更新日期：1997-08-01 00:00:00