Subcellular localization of a small sporulation protein in Bacillus subtilis.

abstract：:We isolated an Escherichia coli mutant strain that suppresses the glycolate-negative phenotype of a strain deficient in both GlcA and LldP transporters of this compound. This suppressing phenotype was assigned to yjcG, a gene whose function was previously unknown, which was found to encode a membrane protein able to t...

journal_title：Journal of bacteriology

authors： Gimenez R,Nuñez MF,Badia J,Aguilar J,Baldoma L

更新日期：2003-11-01 00:00:00

abstract：:A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

journal_title：Journal of bacteriology

authors： Sonstein SA,Hammel JM,Bondi A

更新日期：1971-08-01 00:00:00

abstract：:In an attempt to isolate the superoxide dismutase (SOD) gene from the anaerobic sulfate-reducing bacterium Desulfoarculus baarsii, a DNA fragment was isolated which functionally complemented an Escherichia coli mutant (sodA sodB) deficient in cytoplasmic SODs. This region carries two open reading frames with sequences...

journal_title：Journal of bacteriology

authors： Pianzzola MJ,Soubes M,Touati D

更新日期：1996-12-01 00:00:00

abstract：:Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...

journal_title：Journal of bacteriology

authors： Araújo Alves L,Ganguly T,Mattos-Graner RO,Kajfasz J,Harth-Chu EN,Lemos JA,Abranches J

更新日期：2018-11-06 00:00:00

abstract：:An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of (14)C-PE and (14)C-PE were 61.5% of the total phospholipid, exhibited no significant rate of turnover in either growing or nongrowing cultures. The turnover rates of PE-(14)C and PE-(3...

journal_title：Journal of bacteriology

authors： Makula RA,Finnerty WR

更新日期：1971-09-01 00:00:00

abstract：:Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...

journal_title：Journal of bacteriology

authors： Harrison J,Jamet A,Muglia CI,Van de Sype G,Aguilar OM,Puppo A,Frendo P

更新日期：2005-01-01 00:00:00

abstract：:pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

journal_title：Journal of bacteriology

authors： Zotchev SB,Schrempf H,Hutchinson CR

更新日期：1995-08-01 00:00:00

abstract：:Smith, Rodney F. (University of Maryland, Baltimore), Donald E. Shay, and Norman J. Doorenbos. Antimicrobial action of nitrogen-containing steroids. J. Bacteriol. 85:1295-1299. 1963.-A new group of 16 synthetic nitrogen-containing steroids have been tested against a variety of microorganisms for antimicrobial properti...

journal_title：Journal of bacteriology

authors： SMITH RF,SHAY DE,DOORENBOS NJ

更新日期：1963-06-01 00:00:00

abstract：:Germination of dormant Bacillus subtilis spores with specific nutrient germinants is dependent on a number of inner membrane (IM) proteins, including (i) the GerA, GerB, and GerK germinant receptors (GRs) that respond to nutrient germinants; (ii) the GerD protein, essential for optimal GR function; and (iii) SpoVA pro...

journal_title：Journal of bacteriology

authors： Stewart KA,Setlow P

更新日期：2013-08-01 00:00:00

abstract：:The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...

journal_title：Journal of bacteriology

authors： Salzberg LI,Luo Y,Hachmann AB,Mascher T,Helmann JD

更新日期：2011-10-01 00:00:00

abstract：:Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...

journal_title：Journal of bacteriology

authors： Bulla LA,Bennett GA,Shotwell OL

更新日期：1970-12-01 00:00:00

abstract：:Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...

journal_title：Journal of bacteriology

authors： Kinsinger RF,Kearns DB,Hale M,Fall R

更新日期：2005-12-01 00:00:00

abstract：:Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

journal_title：Journal of bacteriology

authors： Hurek T,Egener T,Reinhold-Hurek B

更新日期：1997-07-01 00:00:00

abstract：:Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...

journal_title：Journal of bacteriology

authors： Faelen M,Toussaint A

更新日期：1978-11-01 00:00:00

abstract：:A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

journal_title：Journal of bacteriology

authors： Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

更新日期：1982-10-01 00:00:00

abstract：:Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1995-10-01 00:00:00

abstract：:Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthes...

journal_title：Journal of bacteriology

authors： Kuo TT,Stocker BA

更新日期：1972-10-01 00:00:00

abstract：:Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

journal_title：Journal of bacteriology

authors： Go MF,Kapur V,Graham DY,Musser JM

更新日期：1996-07-01 00:00:00

abstract：:The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...

journal_title：Journal of bacteriology

authors： Harney SJ,Simopoulos ND,Ikawa M

更新日期：1967-01-01 00:00:00

abstract：:Trichloroethylene (TCE) is the most frequently detected groundwater contaminant, and 1-naphthol is an important chemical manufacturing intermediate. Directed evolution was used to increase the activity of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 for both chlorinated ethenes and naphthalene oxidatio...

journal_title：Journal of bacteriology

authors： Canada KA,Iwashita S,Shim H,Wood TK

更新日期：2002-01-01 00:00:00

abstract：:pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...

journal_title：Journal of bacteriology

authors： Cornelis G,Bennett PM,Grinsted J

更新日期：1976-09-01 00:00:00

abstract：:The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones tha...

journal_title：Journal of bacteriology

authors： Piggot PJ,Curtis CA

更新日期：1987-03-01 00:00:00

abstract：:We previously described mutations in a gene, secB, which have pleiotropic effects on protein export in Escherichia coli. In this paper, we report the isolation of mutants in which the activity of the secB gene was eliminated. Null mutations in secB affected only a subset of exported proteins. Strains carrying these mu...

journal_title：Journal of bacteriology

authors： Kumamoto CA,Beckwith J

更新日期：1985-07-01 00:00:00

abstract：:The intermolecular interactions of the mycobacteriophage Ms6 secretion chaperone with endolysin were characterized. The 384-amino-acid lysin (lysin(384))-binding domain was found to encompass the N-terminal region of Gp1, which is also essential for a lysis phenotype in Escherichia coli. In addition, a GXXXG-like moti...

journal_title：Journal of bacteriology

authors： Catalão MJ,Gil F,Moniz-Pereira J,Pimentel M

更新日期：2011-09-01 00:00:00

abstract：:The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...

journal_title：Journal of bacteriology

authors： Haschke RH,Campbell LL

更新日期：1971-01-01 00:00:00

abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

journal_title：Journal of bacteriology

authors： Huang AM,Lee JI,King SC,Wilson TH

更新日期：1992-08-01 00:00:00

abstract：:The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...

journal_title：Journal of bacteriology

authors： Lynch WH

更新日期：1980-07-01 00:00:00

abstract：:The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...

journal_title：Journal of bacteriology

authors： Garrad RC,Bhattacharjee JK

更新日期：1992-11-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00