Abstract:
:The control of expression of the Bacillus subtilis spoIIA locus was analyzed by titrating gene expression against gene copy number. A plasmid integrated into the B. subtilis chromosome and carrying the spoIIA control region fused to Escherichia coli lacZ was forced to form tandem repeats by the selection of clones that grow on high levels of chloramphenicol, the antibiotic against which the plasmid determines resistance. DNA from the clones was digested with BglII, which did not cut in the reiterated region, and the size of the fragment was determined by orthogonal-field-alternation gel electrophoresis to determine the copy number. Most clones had fairly homogeneous copy numbers. Gene expression was monitored by beta-galactosidase activity. The results indicate that spoIIA was under positive control by a moiety present at about five copies per chromosome. Spore formation was not affected by amplification, so spoIIA-lacZ reiteration did not sequester a molecule required elsewhere for sporulation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Piggot PJ,Curtis CAdoi
10.1128/jb.169.3.1260-1266.1987subject
Has Abstractpub_date
1987-03-01 00:00:00pages
1260-6issue
3eissn
0021-9193issn
1098-5530journal_volume
169pub_type
杂志文章abstract::Cell size at bud initiation shows the same relationship to growth rate in wild-type and petite yeast cells. The nutrient-modulated size control is related to growth rate rather than a change from fermentative to oxidative metabolism. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.1051-1052.1982
更新日期:1982-08-01 00:00:00
abstract::Fatty acid desaturation catalyzed by fatty acid desaturases requires molecular oxygen (O(2)). Saccharomyces cerevisiae cells derepress expression of OLE1 encoding Delta9 fatty acid desaturase under hypoxic conditions to allow more-efficient use of limited O(2). It has been proposed that aerobic conditions lead to repr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.745-751.2001
更新日期:2001-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1772-1779.1995
更新日期:1995-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4741-4746.1997
更新日期:1997-08-01 00:00:00
abstract::The recent emergence of multidrug resistance (MDR) in Acinetobacter baumannii has raised concern in health care settings worldwide. In order to understand the repertoire of resistance determinants and their organization and origins, we compared the genome sequences of three MDR and three drug-susceptible A. baumannii ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00834-08
更新日期:2008-12-01 00:00:00
abstract::Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01008-10
更新日期:2011-01-01 00:00:00
abstract::The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5758-5766.1990
更新日期:1990-10-01 00:00:00
abstract::A key step in the regulation of heat shock genes in Escherichia coli is the stress-dependent degradation of the heat shock promoter-specific sigma(32) subunit of RNA polymerase by the AAA protease, FtsH. Previous studies implicated the C termini of protein substrates, including sigma(32), as degradation signals for AA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.5911-5917.2001
更新日期:2001-10-01 00:00:00
abstract::Experiments are described in which the tensile strength, the initial (Youngs') modulus, and other mechanical properties of the bacterial cell wall were obtained as functions of relative humidity (RH) in the range of 20 to 95%. These properties were deduced from tensile tests on bacterial thread, a fiber consisting of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.197-203.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.11.3439-3446.2004
更新日期:2004-06-01 00:00:00
abstract::FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.3227-3237.2005
更新日期:2005-05-01 00:00:00
abstract::The composition of the molecular species of acidic phospholipids in Escherichia coli B during the late exponential growth phase at 37 degrees C was determined. Two phosphatidyl groups of cardiolipin, the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of cardiolipin, were isolated by limited hydrolysis with p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1047-1051.1980
更新日期:1980-03-01 00:00:00
abstract::DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experime...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00944-10
更新日期:2011-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1850-1856.1994
更新日期:1994-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.3.1189-1199.1975
更新日期:1975-03-01 00:00:00
abstract::Oceaniovalibus guishaninsula, as a representative of a new genus within the family Rhodobacteraceae, was isolated from surface seawater that was sulfidic. Here, we present the draft genome sequence of the type strain, JLT2003(T). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01874-12
更新日期:2012-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5762-5766.1995
更新日期:1995-10-01 00:00:00
abstract::Phase-partitioning studies of the euryhaline bacterium Halomonas elongata demonstrated that the hydrophobic-hydrophilic nature of the cell surface changed as the bacterium grew in different NaCl concentrations. Mid-log-phase cells grown in a high (3.4 M) NaCl concentration were more hydrophilic than were cells grown i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.1.132-135.1988
更新日期:1988-01-01 00:00:00
abstract::Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.176-182.1985
更新日期:1985-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5200-5206.1991
更新日期:1991-08-01 00:00:00
abstract:UNLABELLED:Bacterial cell division is an essential and highly coordinated process. It requires the polymerization of the tubulin homologue FtsZ to form a dynamic ring (Z-ring) at midcell. Z-ring formation relies on a group of FtsZ-associated proteins (Zap) for stability throughout the process of division. In Escherichi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00969-15
更新日期:2016-05-13 00:00:00
abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.404-410.1970
更新日期:1970-05-01 00:00:00
abstract::The 2,647-bp nucleotide sequence of cryptic plasmid pTD1, isolated from the oral spirochete Treponema denticola, was determined. The sequence revealed two open reading frames, A and B, which encode polypeptides of 335 and 235 amino acids, respectively. Open reading frame A shows sequence similarity to genes that encod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2724-2728.1992
更新日期:1992-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.367-379.1976
更新日期:1976-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5959-5970.1995
更新日期:1995-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.2.469-476.1999
更新日期:1999-01-01 00:00:00
abstract::The intestinal pathogen Clostridium difficile is known to grow only within the intestines of mammals, yet little is known about how the bacterium subsists in this environment. In the intestine, C. difficile must contend with innate defenses within the host, such as cationic antimicrobial peptides (CAMPs) produced by t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00166-13
更新日期:2013-06-01 00:00:00
abstract::The effect of CheY and fumarate on switching frequency and rotational bias of the bacterial flagellar motor was analyzed by computer-aided tracking of tethered Escherichia coli. Plots of cells overexpressing CheY in a gutted background showed a bell-shaped correlation curve of Switching frequency and bias centering at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3375-3380.1998
更新日期:1998-07-01 00:00:00
abstract::Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.4935-4939.1995
更新日期:1995-09-01 00:00:00
abstract::The tdh promoter of Escherichia coli is induced seven- to eightfold when cells are grown in the presence of exogenous leucine. A scheme was devised to select mutants that exhibited high constitutive expression of the tdh promoter. The mutations in these strains were shown to lie within a previously identified gene (lr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.19.5944-5953.1991
更新日期:1991-10-01 00:00:00