Abstract:
:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid substitutions in the regions of the putative loops (between transmembrane alpha helices) resulted in a reasonable growth rate on lactose with moderate-to-good transport activity. In one strain (glycine substituted for Trp-10), abnormal sugar recognition was found. The substitution of proline for Trp-33 (in the region of the first alpha helix) showed no activity, while four additional substitutions (lysine, leucine, cysteine, and glutamic acid) showed low activity. Altered sugar specificity was observed when Trp-33 was replaced by serine, glutamine, tyrosine, alanine, histidine, or phenylalanine. It is concluded that Trp-33 may be involved directly or indirectly in sugar recognition.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Huang AM,Lee JI,King SC,Wilson THdoi
10.1128/jb.174.16.5436-5441.1992keywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
5436-41issue
16eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::A delta tyrT::kan mutant from Escherichia coli K-12 (DTK-12) shows a transient growth lag that is caused by glycine starvation (U. Michelsen, M. Bösl, T. Dingermann, and H. Kersten, J. Bacteriol. 171:5987-5994, 1989). The same deletion, transduced into the relA1 spoT1 mutant CA274 to construct strain DTC274, caused co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.1.221-231.1994
更新日期:1994-01-01 00:00:00
abstract::The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01579-14
更新日期:2014-08-01 00:00:00
abstract::Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.861-865.1974
更新日期:1974-11-01 00:00:00
abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.2.329-334.1970
更新日期:1970-08-01 00:00:00
abstract::In Escherichia coli, ClpYQ (HslUV) is a two-component ATP-dependent protease composed of ClpY (HslU), an ATPase with unfolding activity, and ClpQ (HslV), a peptidase. In the ClpYQ proteolytic complex, the hexameric rings of ClpY (HslU) are responsible for protein recognition, unfolding, and translocation into the prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05128-11
更新日期:2011-10-01 00:00:00
abstract::Bacteriophage lambda adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of lambda, to maltose-binding protein. Solid-phase binding assays demonstrated that a purified fusion protein comprising only the last 249 residu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.508-512.2000
更新日期:2000-01-01 00:00:00
abstract::Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.925-932.1985
更新日期:1985-09-01 00:00:00
abstract::Purified bulk tRNA from Methanococcus vanielii (carbon source, formate) showed variation in the modified nucleoside pattern reported for Escherichia coli as analyzed by both ion-exchange and thin-layer chromatography. Ribothymidine and 7-methylguanosine were absent; 1-methyladenosine, 1-methylguanosine, N2-methylguano...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.1.240-250.1978
更新日期:1978-01-01 00:00:00
abstract::Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.2.460-463.1984
更新日期:1984-05-01 00:00:00
abstract::The number and disposition of flagella harbored by eubacteria are regulated by a specific trait successfully maintained over generations. The genes governing the number of flagella in Bacillus subtilis have never been identified, although the ifm locus has long been recognized to influence the motility phenotype of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.15.5356-5366.2005
更新日期:2005-08-01 00:00:00
abstract::Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.1.197-203.1966
更新日期:1966-07-01 00:00:00
abstract::There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1188-1193.1985
更新日期:1985-12-01 00:00:00
abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4594-4597.1988
更新日期:1988-10-01 00:00:00
abstract::CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Furth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.5.1320-1329.2004
更新日期:2004-03-01 00:00:00
abstract::The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00426-09
更新日期:2009-08-01 00:00:00
abstract::The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.40-46.1990
更新日期:1990-01-01 00:00:00
abstract::Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.492-498.1978
更新日期:1978-02-01 00:00:00
abstract::Irradiated L cells infected with Rickettsia tsutsugamushi were studied under the electron microscope to define the morphological growth pattern of the organism. For 2 days after inoculation, no rickettsiae were found either extra- or intracellularly; after 2 days multiple rickettsiae appeared within the host cells wit...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.2.879-892.1983
更新日期:1983-05-01 00:00:00
abstract::Shigella flexneri is a gram-negative bacterium that causes diarrhea and dysentery by invasion and spread through the colonic epithelium. Bacteria spread by assembling actin and other cytoskeletal proteins of the host into "actin tails" at the bacterial pole; actin tail assembly provides the force required to move bact...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.3.869-878.1999
更新日期:1999-02-01 00:00:00
abstract::Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.476-482.1978
更新日期:1978-05-01 00:00:00
abstract::Balish, Edward (Argonne National Laboratory, Argonne, Ill.), and George Svihla. Ultraviolet microscopy of Candida albicans. J. Bacteriol. 92:1812-1820. 1966.-Yeast and mycelial strains of Candida albicans were grown in medium supplemented with sulfur amino acids in an effort to determine factors that control the morph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.6.1812-1820.1966
更新日期:1966-12-01 00:00:00
abstract::Natural isolates of pathogenic bacteria can exhibit a broad range of phenotypic traits. To investigate the molecular mechanisms contributing to such phenotypic variability, we compared the genomes, transcriptomes, and proteomes of two natural isolates of the gram-negative bacterium Burkholderia pseudomallei, the causa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.12.4276-4285.2005
更新日期:2005-06-01 00:00:00
abstract::Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-alpha and SASP-beta) were constructed. These mutants sporulated normally, but the spores lacked either SASP-alpha, SASP-beta, or both proteins. The level of minor SASP di...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.174-178.1986
更新日期:1986-07-01 00:00:00
abstract::Copper (Cu) is a required micronutrient, but it is highly toxic at high concentrations. Therefore, the levels of Cu must be tightly regulated in all living cells. The phagosome of Mycobacterium tuberculosis has been shown to have variable levels of Cu. Previously, we showed that M. tuberculosis contains a copper-sensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01847-07
更新日期:2008-04-01 00:00:00
abstract::A Bacillus subtilis sigM null mutant, lacking the extracytoplasmic function sigma(M) protein, was sensitive to paraquat (PQ), a superoxide-generating reagent, but not to the redox stress-inducing compounds hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, or diamide. Surprisingly, a sigM mutant was only ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.9.2948-2956.2005
更新日期:2005-05-01 00:00:00
abstract::The ops and tps genes of Myxococcus xanthus have ca. 90% DNA and amino acid sequence homology and are in the same orientation separated by a spacer region of only 1.4 kilobases. The products of the two genes were found to cross-react immunologically, and both were capable of Ca2+-dependent self-assembly on the surface...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.121-125.1985
更新日期:1985-07-01 00:00:00
abstract::Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2975-2982.1998
更新日期:1998-06-01 00:00:00
abstract::Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.147.1.91-96.1981
更新日期:1981-07-01 00:00:00
abstract::Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00277-07
更新日期:2007-06-01 00:00:00
abstract::Lüderitz, O. (Max-Planck-Institut für Immunbiologie, Frieburg, Germany), H. J. Risse, H. Schulte-Holthausen, J. L. Strominger, I. W. Sutherland, and O. Westphal. Biochemical studies of the smooth-rough mutation in Salmonella minnesota. J. Bacteriol. 89:343-354. 1965.-A comparative study of the O antigen from the smoot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.2.343-354.1965
更新日期:1965-02-01 00:00:00