Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration.

Abstract:

:Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer the evolutionary history of DSR. A 1.9-kb DNA region encoding most of the alpha and beta subunits of DSR could be recovered only from organisms capable of dissimilatory sulfate reduction with a PCR primer set targeting highly conserved regions in these genes. All DNA sequences obtained were highly similar to one another (49 to 89% identity), and their inferred evolutionary relationships were nearly identical to those inferred on the basis of 16S rRNA. We conclude that the high similarity of bacterial and archaeal DSRs reflects their common origin from a conserved DSR. This ancestral DSR was either present before the split between the domains Bacteria, Archaea, and Eucarya or laterally transferred between Bacteria and Archaea soon after domain divergence. Thus, if the physiological role of the DSR was constant over time, then early ancestors of Bacteria and Archaea already possessed a key enzyme of sulfate and sulfite respiration.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

doi

10.1128/JB.180.11.2975-2982.1998

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

2975-82

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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