Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

abstract：:Methanol:5-hydroxybenzimidazolylcobamide methyltransferase (MT1) is the first of two enzymes required for transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid in Methanosarcina barkeri. MT1 binds the methyl group of methanol to its corrinoid prosthetic group only when the central cobalt atom of th...

journal_title：Journal of bacteriology

authors： Daas PJ,Gerrits KA,Keltjens JT,van der Drift C,Vogels GD

更新日期：1993-03-01 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:When transport of polyamines in Escherichia coli was examined, putrescine excretion was observed under two different physiological conditions: (i) strictly correlated to growth and (ii) following a hyperosmotic shock. Spermidine was not excreted. Characterization of a deletion mutant showed that PotE is not involved i...

journal_title：Journal of bacteriology

authors： Schiller D,Kruse D,Kneifel H,Krämer R,Burkovski A

更新日期：2000-11-01 00:00:00

abstract：:Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

journal_title：Journal of bacteriology

authors： Mickelson MN

更新日期：1967-07-01 00:00:00

abstract：:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

journal_title：Journal of bacteriology

authors： Jin H,Nikolau BJ

更新日期：2012-10-01 00:00:00

abstract：:The O-repeating unit of the Escherichia coli O7-specific lipopolysaccharide is made of galactose, mannose, rhamnose, 4-acetamido-4,6-dideoxyglucose, and N-acetyglucosamine. We have recently characterized the genes involved in the biosynthesis of the sugar precursor GDP-mannose occurring in the E. coli O7:K1 strain VW1...

journal_title：Journal of bacteriology

authors： Marolda CL,Valvano MA

更新日期：1995-10-01 00:00:00

abstract：:Mutations in any one of three genes, kdpA, -B, or -C, in Escherichia coli abolish the activity of Kdp, a multisubunit K+-ATPase that belongs to the P-type ATPase family of cation transporters. We found in this study that expression in vivo of a 135-amino-acid-long N-terminal fragment (KdpA'), less than one-quarter the...

journal_title：Journal of bacteriology

authors： Sardesai AA,Gowrishankar J

更新日期：2001-06-01 00:00:00

abstract：:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

journal_title：Journal of bacteriology

authors： Pinho MG,Filipe SR,de Lencastre H,Tomasz A

更新日期：2001-11-01 00:00:00

abstract：:Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

journal_title：Journal of bacteriology

authors： Baron SF,Franklund CV,Hylemon PB

更新日期：1991-08-01 00:00:00

abstract：:Ramakrishnan, T. (Yale University, New Haven, Conn.), and Edward A. Adelberg. Regulatory mechanisms in the biosynthesis of isoleucine and valine. II. Identification of two operator genes. J. Bacteriol. 89:654-660. 1965.-A tightly clustered set of five structural genes governs the synthesis of the five enzymes of isole...

journal_title：Journal of bacteriology

authors： RAMAKRISHNAN T,ADELBERG EA

更新日期：1965-03-01 00:00:00

abstract：:Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...

journal_title：Journal of bacteriology

authors： Futai M

更新日期：1974-11-01 00:00:00

abstract：:Gel retardation experiments indicated the presence in Pseudomonas aeruginosa cell extracts of an arginine-inducible DNA-binding protein that interacts with the control regions for the car and argF operons, encoding carbamoylphosphate synthetase and anabolic ornithine carbamoyltransferase, respectively. Both enzymes ar...

journal_title：Journal of bacteriology

authors： Park SM,Lu CD,Abdelal AT

更新日期：1997-09-01 00:00:00

abstract：:The ompA gene of Escherichia coli codes for a major protein of the outer membrane. When this gene was moved between various unrelated strains (E. coli K-12 and two clinical isolates of E. coli) by transduction, the gene was expressed very poorly. Recombinants carrying "foreign" genes produced no OmpA protein which cou...

journal_title：Journal of bacteriology

authors： Beher M,Pugsley A,Schnaitman C

更新日期：1980-07-01 00:00:00

abstract：:Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time,...

journal_title：Journal of bacteriology

authors： Tulum I,Yabe M,Uenoyama A,Miyata M

更新日期：2014-05-01 00:00:00

abstract：:The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...

journal_title：Journal of bacteriology

authors： Ofek I,Simpson WA,Beachey EH

更新日期：1982-02-01 00:00:00

abstract：:Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...

journal_title：Journal of bacteriology

authors： Kumar S

更新日期：1976-02-01 00:00:00

abstract：:Pseudomonas putida DOT-T1E is a solvent-resistant strain that is able to grow in the presence of high concentrations of toluene. We have cloned and sequenced the cti gene of this strain, which encodes the cis/trans isomerase, termed Cti, that catalyzes the cis-trans isomerization of esterified fatty acids in phospholi...

journal_title：Journal of bacteriology

authors： Junker F,Ramos JL

更新日期：1999-09-01 00:00:00

abstract：:Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...

journal_title：Journal of bacteriology

authors： Iyehara H,Otsuji N

更新日期：1975-02-01 00:00:00

abstract：:Myxococcus xanthus RB5, a rough-colony-forming, nondispersed growing mutant of strain FBt, forms macroscopic, multicellular masses of radially oriented cells in shake cultures. The cells appear to be held together by slime fibrils. Physical and enzymatic methods to disrupt the spheres were unsuccessful as were attempt...

journal_title：Journal of bacteriology

authors： Burchard RP

更新日期：1975-04-01 00:00:00

abstract：:Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...

journal_title：Journal of bacteriology

authors： Rigel NW,Schwalm J,Ricci DP,Silhavy TJ

更新日期：2012-03-01 00:00:00

abstract：:Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, sugges...

journal_title：Journal of bacteriology

authors： Kapteyn JC,Montijn RC,Dijkgraaf GJ,Van den Ende H,Klis FM

更新日期：1995-07-01 00:00:00

abstract：:Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

journal_title：Journal of bacteriology

authors： Puskas A,Greenberg EP,Kaplan S,Schaefer AL

更新日期：1997-12-01 00:00:00

abstract：:The autolytic capacity of Streptococcus faecium (S. faecalis ATCC 9790) varied during synchronous cell division. This phenomenon was initially observed in rapidly dividing populations (TD=30 to 33 min) synchronized by a combination of induction and size selection techniques. To minimize the problems inherent in studie...

journal_title：Journal of bacteriology

authors： Hinks RP,Daneo-Moore L,Shockman GD

更新日期：1978-02-01 00:00:00

abstract：:Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widesprea...

journal_title：Journal of bacteriology

authors： Tetu SG,Holmes AJ

更新日期：2008-07-01 00:00:00

abstract：:To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By scoring prophage indu...

journal_title：Journal of bacteriology

authors： Dutreix M,Moreau PL,Bailone A,Galibert F,Battista JR,Walker GC,Devoret R

更新日期：1989-05-01 00:00:00

abstract：:The suf operon is composed of four genes (sufB, sufC, sufD, and sufS) and is highly conserved in the genomes of cyanobacteria. Open reading frame sll0088 in Synechocystis sp. strain PCC 6803 is located near the 5' end of the suf operon but is transcribed in the direction opposite that of the suf operon. We previously ...

journal_title：Journal of bacteriology

authors： Wang T,Shen G,Balasubramanian R,McIntosh L,Bryant DA,Golbeck JH

更新日期：2004-02-01 00:00:00

abstract：:Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene-inducing conditions in the presence and absence of bile. Microarray analysis revealed that the expressio...

journal_title：Journal of bacteriology

authors： Cerda-Maira FA,Ringelberg CS,Taylor RK

更新日期：2008-11-01 00:00:00

abstract：:CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more...

journal_title：Journal of bacteriology

authors： Ohtake H,Cervantes C,Silver S

更新日期：1987-08-01 00:00:00

abstract：:The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ft...

journal_title：Journal of bacteriology

authors： Dai K,Lutkenhaus J

更新日期：1991-06-01 00:00:00

abstract：:A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...

journal_title：Journal of bacteriology

authors： Baumann P,Baumann L,Reichelt JL

更新日期：1973-03-01 00:00:00