Bacillus subtilis paraquat resistance is directed by sigmaM, an extracytoplasmic function sigma factor, and is conferred by YqjL and BcrC.

Abstract:

:A Bacillus subtilis sigM null mutant, lacking the extracytoplasmic function sigma(M) protein, was sensitive to paraquat (PQ), a superoxide-generating reagent, but not to the redox stress-inducing compounds hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, or diamide. Surprisingly, a sigM mutant was only sensitive to superoxide-generating compounds with a dipyridyl ring such as PQ, ethyl viologen, benzyl viologen, and diquat but not to menadione, plumbagin, pyrogallol, or nitrofurantoin. Mutational analysis of candidate sigma(M)-regulated genes revealed that both YqjL, a putative hydrolase, and BcrC, a bacitracin resistance protein, were involved in PQ resistance. Expression of yqjL, but not bcrC, from a xylose-inducible promoter restored PQ resistance to the sigM mutant.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Cao M,Moore CM,Helmann JD

doi

10.1128/JB.187.9.2948-2956.2005

keywords:

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

2948-56

issue

9

eissn

0021-9193

issn

1098-5530

pii

187/9/2948

journal_volume

187

pub_type

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