Extent of host deletions associated with bacteriophage P2-mediated eduction.

abstract：:Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...

journal_title：Journal of bacteriology

authors： Paredes-Sabja D,Torres JA,Setlow P,Sarker MR

更新日期：2008-02-01 00:00:00

abstract：:It is thought that in the gram-negative soil bacterium Sinorhizobium meliloti the protein ExoP is involved in biosynthesis of the acidic exopolysaccharide succinoglycan (EPS I). The amounts and compositions of EPS I produced by mutants expressing ExoP proteins characterized by specific amino acid substitutions in the ...

journal_title：Journal of bacteriology

authors： Niemeyer D,Becker A

更新日期：2001-09-01 00:00:00

abstract：:Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

journal_title：Journal of bacteriology

authors： Bouillaut L,Self WT,Sonenshein AL

更新日期：2013-02-01 00:00:00

abstract：:An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...

journal_title：Journal of bacteriology

authors： Li T,Rosazza JP

更新日期：1997-06-01 00:00:00

abstract：:Transposon Tn5-induced C4-dicarboxylate transport mutants of Rhizobium meliloti 2011 which could be complemented by cosmid pRmSC121 were subdivided into two classes. Class I mutants (RMS37 and RMS938) were defective in symbiotic C4-dicarboxylate transport and in nitrogen fixation. They were mutated in the structural g...

journal_title：Journal of bacteriology

authors： Engelke T,Jording D,Kapp D,Pühler A

更新日期：1989-10-01 00:00:00

abstract：:Using an auxotrophic strain of Saccharomyces cerevisiae, we examined the kinetics of ribonucleic acid (RNA) synthesis following inhibition of protein synthesis caused by amino acid starvation or cycloheximide. Removal of a required amino acid immediately stopped net protein synthesis. After a brief lag, RNA synthesis ...

journal_title：Journal of bacteriology

authors： Roth RM,Dampier C

更新日期：1972-02-01 00:00:00

abstract：:Pseudomonas aeruginosa PAO1 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). meta ring cleavage was mediated by 3,4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-d...

journal_title：Journal of bacteriology

authors： Cuskey SM,Olsen RH

更新日期：1988-01-01 00:00:00

abstract：:The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the a...

journal_title：Journal of bacteriology

authors： Ciche TA,Bintrim SB,Horswill AR,Ensign JC

更新日期：2001-05-01 00:00:00

abstract：:A reversal in the relative amounts of the two major species of tyrosine transfer ribonucleic acid (tRNATyr) (I and II) has been previously observed by others during the development of Bacillus subtilis. These species have been purified by benzoylated diethylaminoethyl-cellulose chromatography and were shown to differ ...

journal_title：Journal of bacteriology

authors： Menichi B,Heyman T

更新日期：1976-07-01 00:00:00

abstract：:A characteristic feature of biofilm formation is the production of a protective extracellular polymeric matrix. In the gram-positive bacterium Bacillus subtilis, the biofilm matrix is synthesized by the products of the epsABCDEFGHIJKLMNO operon (hereafter called the eps operon) and yqxM-sipW-tasA loci. Transcription f...

journal_title：Journal of bacteriology

authors： Murray EJ,Strauch MA,Stanley-Wall NR

更新日期：2009-11-01 00:00:00

abstract：:NO, a free radical gas, is the signal for Nitrosomonas europaea cells to switch between different growth modes. At an NO concentration of more than 30 ppm, biofilm formation by N. europaea was induced. NO concentrations below 5 ppm led to a reversal of the biofilm formation, and the numbers of motile and planktonic (m...

journal_title：Journal of bacteriology

authors： Schmidt I,Steenbakkers PJ,op den Camp HJ,Schmidt K,Jetten MS

更新日期：2004-05-01 00:00:00

abstract：:On the basis of the constitutive phenotypes of two catM mutants of Acinetobacter calcoaceticus, the CatM protein was proposed to repress expression of two different loci involved in catechol degradation, catA and catBCIJFD (E. Neidle, C. Hartnett, and L. N. Ornston, J. Bacteriol. 171:5410-5421, 1989). In spite of its ...

journal_title：Journal of bacteriology

authors： Romero-Arroyo CE,Schell MA,Gaines GL 3rd,Neidle EL

更新日期：1995-10-01 00:00:00

abstract：:The regulation by two transcriptional activators of flagellar expression (FlhD and FlhC) and the chemotaxis methyl-accepting protein Aer was studied with glass slide DNA microarrays. An flhD::Kan insertion and an aer deletion were independently introduced into two Escherichia coli K-12 strains, and the effects upon ge...

journal_title：Journal of bacteriology

authors： Prüss BM,Campbell JW,Van Dyk TK,Zhu C,Kogan Y,Matsumura P

更新日期：2003-01-01 00:00:00

abstract：:A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

journal_title：Journal of bacteriology

authors： Smith CJ

更新日期：1985-10-01 00:00:00

abstract：:Clostridium perfringens type B and D isolates produce epsilon-toxin, the third most potent clostridial toxin. The epsilon-toxin gene (etx) is plasmid borne in type D isolates, but etx genetics have been poorly studied in type B isolates. This study reports the first sequencing of any etx plasmid, i.e., pCP8533etx, fro...

journal_title：Journal of bacteriology

authors： Miyamoto K,Li J,Sayeed S,Akimoto S,McClane BA

更新日期：2008-11-01 00:00:00

abstract：:In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...

journal_title：Journal of bacteriology

authors： Kim YJ,Ko IJ,Lee JM,Kang HY,Kim YM,Kaplan S,Oh JI

更新日期：2007-08-01 00:00:00

abstract：:FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

journal_title：Journal of bacteriology

authors： Grantcharova N,Lustig U,Flärdh K

更新日期：2005-05-01 00:00:00

abstract：:Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...

journal_title：Journal of bacteriology

authors： Rosey EL,Kennedy MJ,Petrella DK,Ulrich RG,Yancey RJ Jr

更新日期：1995-10-01 00:00:00

abstract：:Zymomonas mobilis is endowed with two isoenzymes of fermentative alcohol dehydrogenase, a zinc-containing enzyme (ADH I) and an iron-containing enzyme (ADH II). The activity of ADH I remains fully conserved, while ADH II activity decays when anaerobic cultures are shifted to aerobiosis. This differential response depe...

journal_title：Journal of bacteriology

authors： Tamarit J,Cabiscol E,Aguilar J,Ros J

更新日期：1997-02-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...

journal_title：Journal of bacteriology

authors： Peschek GA

更新日期：1983-01-01 00:00:00

abstract：:A new method for measuring the secretion of H(2)O(2) has been based upon an H(2)O(2)-dependent inhibition of catalase by 3-amino-1,2,4-triazole. The conversion of an H(2)O(2)-secretion rate into a catalase inhibition rate amplified a relatively small molar concentration of H(2)O(2) and provided a highly specific and s...

journal_title：Journal of bacteriology

authors： Cohen G,Somerson NL

更新日期：1969-05-01 00:00:00

abstract：:Salmonella typhimurium prototrophs carrying a trpR mutation synthesize tryptophan biosynthetic enzymes constitutively. When feedback inhibition of anthranilate synthetase but not 5'-phosphoribosylpyrophosphate phosphoribosyltransferase activity was by-passed by growing cells on media supplemented with anthranilic acid...

journal_title：Journal of bacteriology

authors： Stuttard C

更新日期：1975-09-01 00:00:00

abstract：:Investigation of the chromosomal region downstream of the lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus revealed the presence of a gene (prtB) encoding a proteinase of 1,946 residues with a predicted molecular mass of 212 kDa. The deduced amino acid sequence showed that PrtB proteinase displays significan...

journal_title：Journal of bacteriology

authors： Gilbert C,Atlan D,Blanc B,Portailer R,Germond JE,Lapierre L,Mollet B

更新日期：1996-06-01 00:00:00

abstract：:H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...

journal_title：Journal of bacteriology

authors： Pilonieta MC,Bodero MD,Munson GP

更新日期：2007-07-01 00:00:00

abstract：:Cultural characteristics and serological relationships of pathogenic marine vibrios isolated from fish in the Pacific Northwest were studied. These organisms were compared with cultures of Vibrio anguillarum, a known fish pathogen. On the basis of morphological and cultural characteristics, the Pacific Northwest strai...

journal_title：Journal of bacteriology

authors： Pacha RE,Kiehn ED

更新日期：1969-12-01 00:00:00

abstract：:Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...

journal_title：Journal of bacteriology

authors： Bizzini A,Zhao C,Budin-Verneuil A,Sauvageot N,Giard JC,Auffray Y,Hartke A

更新日期：2010-02-01 00:00:00

abstract：:Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

journal_title：Journal of bacteriology

authors： Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

更新日期：1995-11-01 00:00:00

abstract：:The quorum-sensing system of Staphylococcus aureus, the accessory gene regulator (Agr) system, is responsible for increased transcription of certain exoprotein genes and decreased transcription of certain cell wall-associated proteins during the postexponential phase of growth. This regulation is important for virulen...

journal_title：Journal of bacteriology

authors： Tseng CW,Zhang S,Stewart GC

更新日期：2004-03-01 00:00:00

abstract：:The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...

journal_title：Journal of bacteriology

authors： Rolfes RJ,Zalkin H

更新日期：1990-10-01 00:00:00