Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.

abstract：:An in vitro transposition system, developed to facilitate gene disruption in Deinococcus radiodurans R1, has been used to inactivate the gene designated dr1819 in uvrA-1(+) and uvrA-1 backgrounds. dr1819 encodes a protein with homology to a UV DNA damage endonuclease expressed by Schizosaccharomyces pombe. Interruptio...

journal_title：Journal of bacteriology

authors： Earl AM,Rankin SK,Kim KP,Lamendola ON,Battista JR

更新日期：2002-02-01 00:00:00

abstract：:The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesse...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1998-12-01 00:00:00

abstract：:XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to clon...

journal_title：Journal of bacteriology

authors： Sirois S,Szatmari G

更新日期：1995-07-01 00:00:00

abstract：:The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...

journal_title：Journal of bacteriology

authors： Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

更新日期：2003-04-01 00:00:00

abstract：UNLABELLED:Over the course of the last 3 decades the role of the second messenger cyclic di-GMP (c-di-GMP) as a master regulator of bacterial physiology was determined. Although the control over c-di-GMP levels via synthesis and breakdown and the allosteric regulation of c-di-GMP over receptor proteins (effectors) and ...

journal_title：Journal of bacteriology

authors： Rotem O,Nesper J,Borovok I,Gorovits R,Kolot M,Pasternak Z,Shin I,Glatter T,Pietrokovski S,Jenal U,Jurkevitch E

更新日期：2015-08-31 00:00:00

abstract：:In gram-negative bacteria, numerous cell functions, including respiration-linked electron transport, have been ascribed to the cytoplasmic membrane. Gram-negative bacteria which use solid substrates (e.g., oxidized manganese or iron) as terminal electron acceptors for anaerobic respiration are presented with a unique ...

journal_title：Journal of bacteriology

authors： Myers CR,Myers JM

更新日期：1992-06-01 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:We are engaged in the genetic and physical characterization of an enterotoxin (Ent) plasmid, Ent P307, which contains genes for the production of a hear-labile and a heat-stable enterotoxin. We are using an Escherichia coli K-12 strain, 711 (P307), constructed by S. Falkow, which contains no other plasmids besides Ent...

journal_title：Journal of bacteriology

authors： Santos DS,Palchaudhuri S,Maas WK

更新日期：1975-12-01 00:00:00

abstract：:Mycoplasma virus L2 is subject to host-specific restriction and modification in Acholeplasma laidlawii strains JA1 and K2. We have examined the DNAs from both host cells and viruses propagated on these strains with respect to susceptibility to cleavage by restriction endonucleases and for DNA base modifications. We sh...

journal_title：Journal of bacteriology

authors： Dybvig K,Swinton D,Maniloff J,Hattman S

更新日期：1982-09-01 00:00:00

abstract：:Enterococcus faecalis is associated with a high proportion of nosocomial infections; however, little is known of the ability of this organism to proliferate in vivo. The ability of RNase B, a model glycoprotein with a single N-glycosylation site occupied by a family of high-mannose-type glycans (Man(5)- to Man(9)-GlcN...

journal_title：Journal of bacteriology

authors： Roberts G,Tarelli E,Homer KA,Philpott-Howard J,Beighton D

更新日期：2000-02-01 00:00:00

abstract：:The relative stability of membrane proteins in Escherichia coli was investigated to determine whether these proteins are degraded at heterogeneous rates and, if so, whether the degradative rates are correlated with the sizes or charges of the proteins. Cells growing in a glucose-limited chemostat with a generation tim...

journal_title：Journal of bacteriology

authors： Schroer DW,St John AC

更新日期：1981-05-01 00:00:00

abstract：:Auxotrophic mutants of Hydrogenomonas eutropha and H. facilis requiring utilizable amino acids were employed to demonstrate the simultaneous utilization of H(2) and an organic substrate for growth. The ratio of the cell yields under dual substrate conditions compared to heterotrophic conditions indicated the relative ...

journal_title：Journal of bacteriology

authors： DeCicco BT,Stukus PE

更新日期：1968-04-01 00:00:00

abstract：:Enterococcus sp. strain C1 is a facultative anaerobe which was coisolated with Citrobacter sp. strain A1 from a sewage oxidation pond. Strain C1 could degrade azo dyes very efficiently via azo reduction and desulfonation in a microaerophilic environment. Here the draft genome sequence of Enterococcus sp. C1 is reporte...

journal_title：Journal of bacteriology

authors： Chan GF,Gan HM,Rashid NA

更新日期：2012-10-01 00:00:00

abstract：:Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...

journal_title：Journal of bacteriology

authors： Stewart PS,White B,Boegli L,Hamerly T,Williamson KS,Franklin MJ,Bothner B,James GA,Fisher S,Vital-Lopez FG,Wallqvist A

更新日期：2019-10-21 00:00:00

abstract：:Cell suspensions of Bacteroides fragilis were allowed to ferment glucose and lactate labeled with (14)C in different positions. The fermentation products, propionate and acetate, were isolated, and the distribution of radioactivity was determined. An analysis of key enzymes of possible pathways was also made. The resu...

journal_title：Journal of bacteriology

authors： Macy JM,Ljungdahl LG,Gottschalk G

更新日期：1978-04-01 00:00:00

abstract：:Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

journal_title：Journal of bacteriology

authors： Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

更新日期：2003-01-01 00:00:00

abstract：:The antibacterial protein toxin colicin V is secreted from Escherichia coli cells by a dedicated export system that is a member of the multicomponent ATP-binding cassette (ABC) transporter family. At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway. In...

journal_title：Journal of bacteriology

authors： Skvirsky RC,Reginald S,Shen X

更新日期：1995-11-01 00:00:00

abstract：:The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...

journal_title：Journal of bacteriology

authors： Irazabal N,Marín I,Amils R

更新日期：1997-03-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Escherichia coli K-12 can utilize a gamma-glutamyl peptide as an amino acid source, for which gamma-glutamyltranspeptidase (EC 2.3.2.2) is essential. We suggest that the gamma-glutamyl linkage of a gamma-glutamyl peptide is hydrolyzed by gamma-glutamyltranspeptidase located in the periplasmic space, and the released a...

journal_title：Journal of bacteriology

authors： Suzuki H,Hashimoto W,Kumagai H

更新日期：1993-09-01 00:00:00

abstract：:SopE is a bacteriophage-encoded effector protein of Salmonella enterica serovar Typhimurium that is translocated into the cytosol of eukaryotic cells by a type III secretion system (TTSS) (W.-D. Hardt, H. Urlaub, and J. E. Galán, Proc. Natl. Acad. Sci. USA 95:2574-2579, 1998; M. W. Wood, R. Rosqvist, P. B. Mullan, M. ...

journal_title：Journal of bacteriology

authors： Lee SH,Galán JE

更新日期：2003-12-01 00:00:00

abstract：:Two bile acid-inducible polypeptides from Eubacterium sp. strain VPI 12708 with molecular weights of 27,000 and approximately 45,000 have previously been shown to be encoded by genes residing on a 2.9-kb EcoRI fragment. We now report the cloning and sequencing of three additional overlapping DNA fragments upstream fro...

journal_title：Journal of bacteriology

authors： Mallonee DH,White WB,Hylemon PB

更新日期：1990-12-01 00:00:00

abstract：:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

journal_title：Journal of bacteriology

authors： Vartak NB,Liu L,Wang BM,Berg CM

更新日期：1991-06-01 00:00:00

abstract：:The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

journal_title：Journal of bacteriology

authors： Laspia MF,Wallace SS

更新日期：1988-08-01 00:00:00

abstract：:A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

journal_title：Journal of bacteriology

authors： Calsou P,Salles B

更新日期：1985-06-01 00:00:00

abstract：:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chr...

journal_title：Journal of bacteriology

authors： Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

更新日期：1990-11-01 00:00:00

abstract：:Rhodobacter sphaeroides maintained intracellular ammonium pools of 1.1 to 2.6 mM during growth in several fixed nitrogen sources as well as during diazotrophic growth. Addition of 0.15 mM NH4+ to washed, nitrogen-free cell suspensions was followed by linear uptake of NH4+ from the medium and transient formation of int...

journal_title：Journal of bacteriology

authors： Cordts ML,Gibson J

更新日期：1987-04-01 00:00:00

abstract：:The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it...

journal_title：Journal of bacteriology

authors： DiRienzo JM,MacLeod RA

更新日期：1978-10-01 00:00:00

abstract：:The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

journal_title：Journal of bacteriology

authors： Merryweather A,Barth PT,Wilkins BM

更新日期：1986-07-01 00:00:00

abstract：:Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

journal_title：Journal of bacteriology

authors： HARPER MK,CURRAN HR,PALLANSCH MJ

更新日期：1964-11-01 00:00:00