Assay and purification of S-adenosyl-L-methionine:precorrin-2 methyltransferase from Pseudomonas denitrificans.

Abstract:

:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chromatography on DEAE-Trisacryl, hydroxyapatite, and Mono Q HR purified the enzyme about 110-fold, with a 28% yield. For enzyme purification and characterization, a coupled-enzyme assay was developed which generated in situ the highly oxygen-sensitive substrate, precorrin-2, from delta-aminolevulinic acid. Evidence is given that the chemically reduced form of sirohydrochlorin (dihydrosirohydrochlorin) is methylated at C-20 to precorrin-3 by pure SP2MT. No subsequent SP2MT-dependent methylation reaction of precorrin-3 was detected. The native enzyme has an apparent molecular weight of 53,000, as estimated by gel filtration, and consists of two identical subunits of Mr 26,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Stepwise Edman degradation provided the N-terminal sequence of the first 17 amino acids.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche F

doi

10.1128/jb.172.11.6245-6251.1990

subject

Has Abstract

pub_date

1990-11-01 00:00:00

pages

6245-51

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

杂志文章
  • Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles.

    abstract::The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.265-275.1977

    authors: Bassford PJ Jr,Kadner RJ,Schnaitman CA

    更新日期:1977-01-01 00:00:00

  • Augmented expression of polysaccharide intercellular adhesin in a defined Staphylococcus epidermidis mutant with the small-colony-variant phenotype.

    abstract::While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00160-07

    authors: Al Laham N,Rohde H,Sander G,Fischer A,Hussain M,Heilmann C,Mack D,Proctor R,Peters G,Becker K,von Eiff C

    更新日期:2007-06-01 00:00:00

  • Comparative structural and molecular characterization of ribitol-5-phosphate-containing Streptococcus oralis coaggregation receptor polysaccharides.

    abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01532-08

    authors: Yang J,Ritchey M,Yoshida Y,Bush CA,Cisar JO

    更新日期:2009-03-01 00:00:00

  • Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition.

    abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.808-820.1978

    authors: Harley JB,Santangelo GM,Rasmussen H,Goldfine H

    更新日期:1978-06-01 00:00:00

  • Arm-in-Arm Response Regulator Dimers Promote Intermolecular Signal Transduction.

    abstract:UNLABELLED:Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00872-15

    authors: Baker AW,Satyshur KA,Moreno Morales N,Forest KT

    更新日期:2016-03-31 00:00:00

  • Genome sequence of the agar-degrading marine bacterium Alteromonadaceae sp. strain G7.

    abstract::Here, we present the high-quality draft genome sequence of the agar-degrading marine gammaproteobacterium Alteromonadaceae sp. strain G7, which was isolated from coastal seawater to be utilized as a bioresource for production of agar-derived biofuels. The 3.91-Mb genome contains a number of genes encoding algal polysa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01931-12

    authors: Kwak MJ,Song JY,Kim BK,Chi WJ,Kwon SK,Choi S,Chang YK,Hong SK,Kim JF

    更新日期:2012-12-01 00:00:00

  • The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation.

    abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.525-530.1990

    authors: Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

    更新日期:1990-02-01 00:00:00

  • Conjugational recombination in resolvase-deficient ruvC mutants of Escherichia coli K-12 depends on recG.

    abstract::ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5414-5418.1991

    authors: Lloyd RG

    更新日期:1991-09-01 00:00:00

  • Bacillus subtilis locus encoding a killer protein and its antidote.

    abstract::We have isolated mutations that block sporulation after formation of the polar septum in Bacillus subtilis. These mutations were mapped to the two genes of a new locus, spoIIS. Inactivation of the second gene, spoIISB, decreases sporulation efficiency by 4 orders of magnitude. Inactivation of the first gene, spoIISA, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3574-3581.2001

    authors: Adler E,Barák I,Stragier P

    更新日期:2001-06-01 00:00:00

  • Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles.

    abstract::We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.722-727.1971

    authors: Freifelder D,Folkmanis A,Kirschner I

    更新日期:1971-03-01 00:00:00

  • Transduction in Bacillus subtilis.

    abstract::Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.106-111.1962

    authors: THORNE CB

    更新日期:1962-01-01 00:00:00

  • cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.

    abstract::Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3080-3088.1988

    authors: Kuroda MI,Henner D,Yanofsky C

    更新日期:1988-07-01 00:00:00

  • Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes.

    abstract::The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2292-2303.1993

    authors: Neidle EL,Kaplan S

    更新日期:1993-04-01 00:00:00

  • NBU1, a mobilizable site-specific integrated element from Bacteroides spp., can integrate nonspecifically in Escherichia coli.

    abstract::NBU1 is an integrated Bacteroides element that can he mobilized from Bacteroides donors to Bacteroides recipients. Previous studies have shown that a plasmid carrying the internal mobilization region of NBU1 could be transferred by conjugation from Bacteroides thetaiotaomicron to Escherichia coli. In this report, we s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.12.3601-3607.1996

    authors: Shoemaker NB,Wang GR,Salyers AA

    更新日期:1996-06-01 00:00:00

  • Presence of polyribosomes in condiospores of Botryodiplodia theobromae harvested with nonaqueous solvents.

    abstract::Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.3.1394-1395.1975

    authors: Brambl R

    更新日期:1975-06-01 00:00:00

  • Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli.

    abstract::In Escherichia coli, cell division is mediated by the concerted action of about 12 proteins that assemble at the division site to presumably form a complex called the divisome. Among these essential division proteins, the multimodular class B penicillin-binding protein 3 (PBP3), which is specifically involved in septa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.18.6110-6117.2004

    authors: Piette A,Fraipont C,Den Blaauwen T,Aarsman ME,Pastoret S,Nguyen-Distèche M

    更新日期:2004-09-01 00:00:00

  • Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica.

    abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1144-1155.1973

    authors: Baumann P,Baumann L,Reichelt JL

    更新日期:1973-03-01 00:00:00

  • Fibronectin binding to the Salmonella enterica serotype Typhimurium ShdA autotransporter protein is inhibited by a monoclonal antibody recognizing the A3 repeat.

    abstract::ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.15.4931-4939.2004

    authors: Kingsley RA,Abi Ghanem D,Puebla-Osorio N,Keestra AM,Berghman L,Bäumler AJ

    更新日期:2004-08-01 00:00:00

  • Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa.

    abstract::The slime polysaccharides produced by Pseudomonas aeruginosa isolated from a variety of human infections were investigated. Slime production in culture seemed optimal when adequate amounts of carbohydrate were present and under conditions of either high osmotic pressure or inadequate protein supply. The polysaccharide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.915-924.1973

    authors: Evans LR,Linker A

    更新日期:1973-11-01 00:00:00

  • Draft genome sequence of the flocculating Zymomonas mobilis strain ZM401 (ATCC 31822).

    abstract::Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01947-12

    authors: Zhao N,Bai Y,Zhao XQ,Yang ZY,Bai FW

    更新日期:2012-12-01 00:00:00

  • Characterization of the lipopolysaccharide from a Rhizobium phaseoli mutant that is defective in infection thread development.

    abstract::The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that it has an altered LP...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.4923-4928.1987

    authors: Carlson RW,Kalembasa S,Turowski D,Pachori P,Noel KD

    更新日期:1987-11-01 00:00:00

  • Comparison of the activities of extracts of Escherichia coli and Salmonella typhimurium in amino acid incorporation.

    abstract::We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.757-763.1973

    authors: Bassel BA,Curry ME

    更新日期:1973-11-01 00:00:00

  • Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome Reveals a Compartmentalization Bias toward the Bacterial Surface.

    abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00658-16

    authors: Dowdell AS,Murphy MD,Azodi C,Swanson SK,Florens L,Chen S,Zückert WR

    更新日期:2017-02-28 00:00:00

  • Cell death in Escherichia coli dnaE(Ts) mutants incubated at a nonpermissive temperature is prevented by mutation in the cydA gene.

    abstract::Cells of the Escherichia coli dnaE(Ts) dnaE74 and dnaE486 mutants die after 4 h of incubation at 40 degrees C in Luria-Bertani medium. Cell death is preceded by elongation, is inhibited by chloramphenicol, tetracycline, or rifampin, and is dependent on cell density. Cells survive at 40 degrees C when they are incubate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.7.2147-2155.2004

    authors: Strauss B,Kelly K,Dincman T,Ekiert D,Biesieda T,Song R

    更新日期:2004-04-01 00:00:00

  • Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli.

    abstract::Cells of Escherichia coli take up vitamin B(12) (cyano-cobalamin [CN-Cbl]) and iron chelates by use of sequential active transport processes. Transport of CN-Cbl across the outer membrane and its accumulation in the periplasm is mediated by the TonB-dependent transporter BtuB. Transport across the cytoplasmic membrane...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.3.706-717.2002

    authors: Cadieux N,Bradbeer C,Reeger-Schneider E,Köster W,Mohanty AK,Wiener MC,Kadner RJ

    更新日期:2002-02-01 00:00:00

  • Cell wall constituents of Leuconostoc citrovorum and Leuconostoc mesenteroides.

    abstract::The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.273-277.1967

    authors: Harney SJ,Simopoulos ND,Ikawa M

    更新日期:1967-01-01 00:00:00

  • Functional analysis of OleY L-oleandrosyl 3-O-methyltransferase of the oleandomycin biosynthetic pathway in Streptomyces antibioticus.

    abstract::Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.183.18.5358-5363.2001

    authors: Rodríguez L,Rodríguez D,Olano C,Braña AF,Méndez C,Salas JA

    更新日期:2001-09-01 00:00:00

  • Contribution of σ70 and σN Factors to Expression of Class II pilE in Neisseria meningitidis.

    abstract::Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00170-19

    authors: Lobanovska M,Tang CM,Exley RM

    更新日期:2019-09-20 00:00:00

  • Proteolytic adaptor for transfer-messenger RNA-tagged proteins from alpha-proteobacteria.

    abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01387-06

    authors: Lessner FH,Venters BJ,Keiler KC

    更新日期:2007-01-01 00:00:00

  • ExoS controls the cell contact-mediated switch to effector secretion in Pseudomonas aeruginosa.

    abstract::Type III secretion is used by many gram-negative bacterial pathogens to directly deliver protein toxins (effectors) into targeted host cells. In all cases, secretion of effectors is triggered by host cell contact, although the mechanism is unclear. In Pseudomonas aeruginosa, expression of all type III secretion-relate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01553-07

    authors: Cisz M,Lee PC,Rietsch A

    更新日期:2008-04-01 00:00:00