Abstract:
:S-Adenosyl-L-methionine:precorrin-2 methyltransferase (SP2MT), which catalyzes the C-20 methylation of precorrin-2 to precorrin-3, was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans derived from a cobalamin-overproducing strain. Ammonium sulfate fractionation followed by chromatography on DEAE-Trisacryl, hydroxyapatite, and Mono Q HR purified the enzyme about 110-fold, with a 28% yield. For enzyme purification and characterization, a coupled-enzyme assay was developed which generated in situ the highly oxygen-sensitive substrate, precorrin-2, from delta-aminolevulinic acid. Evidence is given that the chemically reduced form of sirohydrochlorin (dihydrosirohydrochlorin) is methylated at C-20 to precorrin-3 by pure SP2MT. No subsequent SP2MT-dependent methylation reaction of precorrin-3 was detected. The native enzyme has an apparent molecular weight of 53,000, as estimated by gel filtration, and consists of two identical subunits of Mr 26,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Stepwise Edman degradation provided the N-terminal sequence of the first 17 amino acids.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Thibaut D,Couder M,Crouzet J,Debussche L,Cameron B,Blanche Fdoi
10.1128/jb.172.11.6245-6251.1990subject
Has Abstractpub_date
1990-11-01 00:00:00pages
6245-51issue
11eissn
0021-9193issn
1098-5530journal_volume
172pub_type
杂志文章abstract::The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.265-275.1977
更新日期:1977-01-01 00:00:00
abstract::While coagulase-negative staphylococci (CoNS), with their ability to form a thick, multilayered biofilm on foreign bodies, have been identified as the major cause of implant-associated infections, no data are available about biofilm formation by staphylococcal small-colony variants (SCVs). In the past years, a number ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00160-07
更新日期:2007-06-01 00:00:00
abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01532-08
更新日期:2009-03-01 00:00:00
abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.808-820.1978
更新日期:1978-06-01 00:00:00
abstract:UNLABELLED:Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00872-15
更新日期:2016-03-31 00:00:00
abstract::Here, we present the high-quality draft genome sequence of the agar-degrading marine gammaproteobacterium Alteromonadaceae sp. strain G7, which was isolated from coastal seawater to be utilized as a bioresource for production of agar-derived biofuels. The 3.91-Mb genome contains a number of genes encoding algal polysa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01931-12
更新日期:2012-12-01 00:00:00
abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.525-530.1990
更新日期:1990-02-01 00:00:00
abstract::ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5414-5418.1991
更新日期:1991-09-01 00:00:00
abstract::We have isolated mutations that block sporulation after formation of the polar septum in Bacillus subtilis. These mutations were mapped to the two genes of a new locus, spoIIS. Inactivation of the second gene, spoIISB, decreases sporulation efficiency by 4 orders of magnitude. Inactivation of the first gene, spoIISA, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.12.3574-3581.2001
更新日期:2001-06-01 00:00:00
abstract::We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The cau...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.722-727.1971
更新日期:1971-03-01 00:00:00
abstract::Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.106-111.1962
更新日期:1962-01-01 00:00:00
abstract::Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.3080-3088.1988
更新日期:1988-07-01 00:00:00
abstract::The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.8.2292-2303.1993
更新日期:1993-04-01 00:00:00
abstract::NBU1 is an integrated Bacteroides element that can he mobilized from Bacteroides donors to Bacteroides recipients. Previous studies have shown that a plasmid carrying the internal mobilization region of NBU1 could be transferred by conjugation from Bacteroides thetaiotaomicron to Escherichia coli. In this report, we s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3601-3607.1996
更新日期:1996-06-01 00:00:00
abstract::Polyribosomes detected in extracts of spores harvested with water also were found in extracts prepared from spores harvested with nonaqueous fluids. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.1394-1395.1975
更新日期:1975-06-01 00:00:00
abstract::In Escherichia coli, cell division is mediated by the concerted action of about 12 proteins that assemble at the division site to presumably form a complex called the divisome. Among these essential division proteins, the multimodular class B penicillin-binding protein 3 (PBP3), which is specifically involved in septa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.18.6110-6117.2004
更新日期:2004-09-01 00:00:00
abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1144-1155.1973
更新日期:1973-03-01 00:00:00
abstract::ShdA is a large outer membrane protein of the autotransporter family whose passenger domain binds the extracellular matrix proteins fibronectin and collagen I, possibly by mimicking the host ligand heparin. The ShdA passenger domain consists of approximately 1,500 amino acid residues that can be divided into two regio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.15.4931-4939.2004
更新日期:2004-08-01 00:00:00
abstract::The slime polysaccharides produced by Pseudomonas aeruginosa isolated from a variety of human infections were investigated. Slime production in culture seemed optimal when adequate amounts of carbohydrate were present and under conditions of either high osmotic pressure or inadequate protein supply. The polysaccharide...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.915-924.1973
更新日期:1973-11-01 00:00:00
abstract::Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01947-12
更新日期:2012-12-01 00:00:00
abstract::The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that it has an altered LP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.4923-4928.1987
更新日期:1987-11-01 00:00:00
abstract::We have compared the amino acid incorporating activities of extracts of Escherichia coli and Salmonella typhimurium in in vitro protein-synthesizing systems directed by bacterial messenger ribonucleic acid (mRNA) of both species and by the genomes of coliphages Qbeta and f2. E. coli and S. typhimurium extracts transla...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.116.2.757-763.1973
更新日期:1973-11-01 00:00:00
abstract::The Lyme disease spirochete Borrelia burgdorferi is unique among bacteria in its large number of lipoproteins that are encoded by a small, exceptionally fragmented, and predominantly linear genome. Peripherally anchored in either the inner or outer membrane and facing either the periplasm or the external environment, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00658-16
更新日期:2017-02-28 00:00:00
abstract::Cells of the Escherichia coli dnaE(Ts) dnaE74 and dnaE486 mutants die after 4 h of incubation at 40 degrees C in Luria-Bertani medium. Cell death is preceded by elongation, is inhibited by chloramphenicol, tetracycline, or rifampin, and is dependent on cell density. Cells survive at 40 degrees C when they are incubate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.2147-2155.2004
更新日期:2004-04-01 00:00:00
abstract::Cells of Escherichia coli take up vitamin B(12) (cyano-cobalamin [CN-Cbl]) and iron chelates by use of sequential active transport processes. Transport of CN-Cbl across the outer membrane and its accumulation in the periplasm is mediated by the TonB-dependent transporter BtuB. Transport across the cytoplasmic membrane...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.706-717.2002
更新日期:2002-02-01 00:00:00
abstract::The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.273-277.1967
更新日期:1967-01-01 00:00:00
abstract::Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.183.18.5358-5363.2001
更新日期:2001-09-01 00:00:00
abstract::Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00170-19
更新日期:2019-09-20 00:00:00
abstract::We have identified an analog of SspB, the proteolytic adaptor for transfer-messenger RNA (tmRNA)-tagged proteins, in Caulobacter crescentus. C. crescentus SspB shares limited sequence similarity with Escherichia coli SspB but binds the tmRNA tag in vitro and is required for optimal proteolysis of tagged proteins in vi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01387-06
更新日期:2007-01-01 00:00:00
abstract::Type III secretion is used by many gram-negative bacterial pathogens to directly deliver protein toxins (effectors) into targeted host cells. In all cases, secretion of effectors is triggered by host cell contact, although the mechanism is unclear. In Pseudomonas aeruginosa, expression of all type III secretion-relate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01553-07
更新日期:2008-04-01 00:00:00