Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles.

abstract：:The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

journal_title：Journal of bacteriology

authors： Kim SW,Kim CY,Benisek WF,Choi KY

更新日期：1994-11-01 00:00:00

abstract：:Streptococcus faecalis transposon Tn917 was cloned in Escherichia coli on plasmid vector pBR325. The erythromycin resistance determinant of Tn917 was not expressed in the E. coli background. The nucleotide sequence of Tn917 was determined and found to be 5,257 base pairs in length. Six open reading frames (ORFs) were ...

journal_title：Journal of bacteriology

authors： Shaw JH,Clewell DB

更新日期：1985-11-01 00:00:00

abstract：:Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...

journal_title：Journal of bacteriology

authors： Kloft N,Forchhammer K

更新日期：2005-10-01 00:00:00

abstract：:Some strains of Bradyrhizobium japonicum have the ability to catabolize indole-3-acetic acid. Indoleacetic acid (IAA), 4-chloro-IAA (4-Cl-IAA), and 5-Cl-IAA were metabolized to different extents by strains 61A24 and 110. Metabolites were isolated and analyzed by high-performance liquid chromatography and conventional ...

journal_title：Journal of bacteriology

authors： Jensen JB,Egsgaard H,Van Onckelen H,Jochimsen BU

更新日期：1995-10-01 00:00:00

abstract：:Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

journal_title：Journal of bacteriology

authors： MOULDER JW,NOVOSEL DL,TRIBBY II

更新日期：1965-01-01 00:00:00

abstract：:A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

journal_title：Journal of bacteriology

authors： Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

更新日期：1995-05-01 00:00:00

abstract：:Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...

journal_title：Journal of bacteriology

authors： Jones DT,Woods DR

更新日期：1981-01-01 00:00:00

abstract：:The decline in colony-forming ability observed during tryptophan starvation of Bacillus subtilis auxotrophs is a concentration-dependent phenomenon. It does not manifest itself when the initial cell concentration is 10(6) cells/ml or lower. This property has been used to test the killing activity of different fraction...

journal_title：Journal of bacteriology

authors： Barlati S,Majerfeld I

更新日期：1970-02-01 00:00:00

abstract：:The diversity of regulatory systems encoded by bacteria provides an indication of the variety of stresses and interactions that these organisms encounter in nature. We have been investigating how the plant pathogen Pseudomonas syringae pv. tomato DC3000 responds to iron limitation and have focused on the iron starvati...

journal_title：Journal of bacteriology

authors： Markel E,Maciak C,Butcher BG,Myers CR,Stodghill P,Bao Z,Cartinhour S,Swingle B

更新日期：2011-10-01 00:00:00

abstract：:The bacteriophage P22-based challenge-phage system was used to study the binding of Xis and FIS to their sites in attP of bacteriophage lambda. Challenge phages were constructed that contained the X1, X2, and F sites within the P22 Pant promoter, which is required for expression of antirepressor. If Xis and FIS bind t...

journal_title：Journal of bacteriology

authors： Numrych TE,Gumport RI,Gardner JF

更新日期：1991-10-01 00:00:00

abstract：:Studies of biosynthetic pathways with purines as substrates showed that Methanococcus vannielii was capable of degrading xanthine to an extent that several of the carbon atoms were converted to CO2. Experiments to determine whether this catabolic activity could satisfy the entire nitrogen requirement for growth of M. ...

journal_title：Journal of bacteriology

authors： DeMoll E,Tsai L

更新日期：1986-08-01 00:00:00

abstract：:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

journal_title：Journal of bacteriology

authors： Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

更新日期：1988-08-01 00:00:00

abstract：:An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the...

journal_title：Journal of bacteriology

authors： Kong D,Masker W

更新日期：1994-10-01 00:00:00

abstract：:The overlapping fdhA and fdhB genes of Methanobacterium formicicum, which encode the alpha and beta subunits, respectively, of formate dehydrogenase, were cotranscribed as part of an approximately 4.5-kb transcript. An additional gene (fdhC) upstream of fdhA was cotranscribed with fdhA and fdhB. The deduced amino acid...

journal_title：Journal of bacteriology

authors： White WB,Ferry JG

更新日期：1992-08-01 00:00:00

abstract：:The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized. In addition to the known cellulose (cel) and acidic exopolysaccharide (EPS) (pss) genes, w...

journal_title：Journal of bacteriology

authors： Williams A,Wilkinson A,Krehenbrink M,Russo DM,Zorreguieta A,Downie JA

更新日期：2008-07-01 00:00:00

abstract：:Neely, W. Brock (The Dow Chemical Co., Midland, Mich.). Action of formaldehyde on microorganisms. I. Correlation of activity with formaldehyde metabolism. J. Bacteriol. 85:1028-1031. 1963.-The action of formaldehyde in concentrations sublethal for the culture appeared to be at least twofold. The first phase caused a d...

journal_title：Journal of bacteriology

authors： NEELY WB

更新日期：1963-05-01 00:00:00

abstract：:The ESX-1 (ESAT-6 system 1) secretion system plays a conserved role in the virulence of diverse mycobacterial pathogens, including the human pathogen Mycobacterium tuberculosis and M. marinum, an environmental mycobacterial species. The ESX-1 system promotes the secretion of protein virulence factors to the extracytop...

journal_title：Journal of bacteriology

authors： Bosserman RE,Nicholson KR,Champion MM,Champion PA

更新日期：2019-06-21 00:00:00

abstract：:Bacterial biofilms are important in natural settings, biotechnology, and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread...

journal_title：Journal of bacteriology

authors： Bartolini M,Cogliati S,Vileta D,Bauman C,Rateni L,Leñini C,Argañaraz F,Francisco M,Villalba JM,Steil L,Völker U,Grau R

更新日期：2018-12-20 00:00:00

abstract：:Conditions have been established for the preparation of spheroplasts of Saccharomyces cerevisiae which are able to increase their net content of protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), several-fold upon incubation in a medium stabilized with 1 m sorbitol. The rate of RNA and protein synthesis...

journal_title：Journal of bacteriology

authors： Hutchison HT,Hartwell LH

更新日期：1967-11-01 00:00:00

abstract：:Resistance to the antifolates methasquin and amethopterin has been studied in new strains of Streptococcus faecium var. durans. Two methasquin-resistant strains (SF/MQ, SF/MQ(T)) and an amethopterin-resistant strain (SF/AM) were selected independently from the wild-type S. faecium var. durans (SF/O). SF/MQ(T) is a thy...

journal_title：Journal of bacteriology

authors： Rader JI,Hutchison DJ

更新日期：1972-04-01 00:00:00

abstract：:Pigmentation mutants of Pseudomonas aeruginosa, selected by observed visual differences in coloration from the wild-type strain, were examined for altered patterns of phenazine synthesis. Three classes of mutants that were incapable of pyocyanine production were identified. Pigmentation patterns that were found to cha...

journal_title：Journal of bacteriology

authors： Byng GS,Eustice DC,Jensen RA

更新日期：1979-06-01 00:00:00

abstract：:DivIVA is involved in Bacillus subtilis cell division and is located at the cell poles. Previous experiments suggested that the cell division proteins FtsZ and PBP 2B are required for polar targeting of DivIVA. By using outgrowing spores, we show that DivIVA accumulates at the cell poles independent of the presence of...

journal_title：Journal of bacteriology

authors： Hamoen LW,Errington J

更新日期：2003-01-01 00:00:00

abstract：:Most isolates of Salmonella enterica serovar Typhimurium contain a 90-kb virulence plasmid. This plasmid is reported to be mobilizable but nonconjugative. However, we have determined that the virulence plasmid of strains LT2, 14028, and SR-11 is indeed self-transmissible. The plasmid of strain SL1344 is not. Optimal c...

journal_title：Journal of bacteriology

authors： Ahmer BM,Tran M,Heffron F

更新日期：1999-02-01 00:00:00

abstract：:Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...

journal_title：Journal of bacteriology

authors： Maynard A,Butler NL,Ito T,da Silva AJ,Murai M,Chen T,Koffas MAG,Miyoshi H,Barquera B

更新日期：2019-05-08 00:00:00

abstract：:To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

journal_title：Journal of bacteriology

authors： Hatfull GF,Joyce CM

更新日期：1986-06-01 00:00:00

abstract：:The mechanisms by which Legionella pneumophila, a facultative intracellular parasite and the agent of Legionnaires' disease, acquires iron are largely unexplained. Several earlier studies indicated that L. pneumophila does not elaborate siderophores. However, we now present evidence that supernatants from L. pneumophi...

journal_title：Journal of bacteriology

authors： Liles MR,Scheel TA,Cianciotto NP

更新日期：2000-02-01 00:00:00

abstract：:A segment of early RNA from Bacillus subtilis bacteriophage SP82 was shown to function as a 5' stabilizer in B. subtilis. Several heterologous RNA sequences were stabilized by the presence of the SP82 sequence at the 5' end, and expression of downstream coding sequences was increased severalfold. The SP82 RNA segment ...

journal_title：Journal of bacteriology

authors： Hue KK,Cohen SD,Bechhofer DH

更新日期：1995-06-01 00:00:00

abstract：:The medium in which Rec(+) strains of Escherichia coli K-12 are grown affected their sensitivity to treatment with methyl methane sulfonate (MMS). Rec(+) cells grown to the stationary phase in glucose-enriched nutrient broth (GNB) were more resistant to MMS than cells grown in nutrient broth (NB). The repair of MMS-in...

journal_title：Journal of bacteriology

authors： Scudiero DA,Friesen BS,Baptist JE

更新日期：1973-04-01 00:00:00

abstract：:The genes for protein synthesis elongation factors Tu and G were cloned from the cyanobacterium Anacystis nidulans. The locations of these genes were mapped within the cloned DNA fragment by hybridization with Escherichia coli probes. The organization of the cloned fragment and the DNA flanking it in the A. nidulans c...

journal_title：Journal of bacteriology

authors： Mickel FS,Spremulli LL

更新日期：1986-04-01 00:00:00

abstract：:The role of the carboxy terminus of the Escherichia coli cell division protein FtsA in bacterial division has been studied by making a series of short sequential deletions spanning from residue 394 to 420. Deletions as short as 5 residues destroy the biological function of the protein. Residue W415 is essential for th...

journal_title：Journal of bacteriology

authors： Yim L,Vandenbussche G,Mingorance J,Rueda S,Casanova M,Ruysschaert JM,Vicente M

更新日期：2000-11-01 00:00:00