Abstract:
:A segment of early RNA from Bacillus subtilis bacteriophage SP82 was shown to function as a 5' stabilizer in B. subtilis. Several heterologous RNA sequences were stabilized by the presence of the SP82 sequence at the 5' end, and expression of downstream coding sequences was increased severalfold. The SP82 RNA segment encodes a B. subtilis RNase III cleavage site, but cleavage by B. subtilis RNase III was not required for stabilization. The sequence that specifies 5' stabilizer function was localized to a polypurine sequence that resembles a ribosome binding site. The ability of the SP82 sequence to stabilize downstream RNA was dependent on its position relative to the 5' end of the RNA. These results demonstrate the existence of a new type of 5' stabilizer in B. subtilis and indicate that attack at the 5' end is a principal mechanism for initiation of mRNA decay in B. subtilis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Hue KK,Cohen SD,Bechhofer DHdoi
10.1128/jb.177.12.3465-3471.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
3465-71issue
12eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1295-1297.1966
更新日期:1966-03-01 00:00:00
abstract::A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identifi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5968-5979.1990
更新日期:1990-10-01 00:00:00
abstract::Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.3.874-881.2006
更新日期:2006-02-01 00:00:00
abstract::The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T(53)) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T(531)) cultured in BHI without sucrose; and (iii) an unstable...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.73-75.1973
更新日期:1973-07-01 00:00:00
abstract::IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00989-08
更新日期:2009-02-01 00:00:00
abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1153-1161.1982
更新日期:1982-09-01 00:00:00
abstract::PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.23.6592-6597.2000
更新日期:2000-12-01 00:00:00
abstract::Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.940-943.1969
更新日期:1969-02-01 00:00:00
abstract::The deoxyribonucleic acid base composition (percent guanine + cytosine [GC]) was determined for 29 strains, representing 18 species of the genus Kluyveromyces. It was concluded that on the basis of GC content (47.4%) and other properties K. veronae occupies an uncertain position in the genus Kluyveromyces. The GC cont...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.2.481-487.1972
更新日期:1972-08-01 00:00:00
abstract::An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.5.2063-2069.1988
更新日期:1988-05-01 00:00:00
abstract::Several environmental stresses have been demonstrated to increase polysaccharide intercellular adhesin (PIA) synthesis and biofilm formation by the human pathogens Staphylococcus aureus and Staphylococcus epidermidis. In this study we characterized an adaptive response of S. aureus SA113 to nitrite-induced stress and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00598-07
更新日期:2007-11-01 00:00:00
abstract::Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01392-12
更新日期:2012-11-01 00:00:00
abstract::Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.712-717.1965
更新日期:1965-03-01 00:00:00
abstract::Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.595-603.1971
更新日期:1971-02-01 00:00:00
abstract::This study examined the catabolism of xanthone by an Arthrobacter sp. (strain GFB100) capable of growth on xanthone as its main source of carbon and energy. An early catabolic intermediate was 3,4-dihydroxyxanthone. This compound was isolated from the growth medium of a mutant strain of the Arthrobacter sp. which lack...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.3.818-827.1986
更新日期:1986-09-01 00:00:00
abstract::The penicillin-binding proteins (PBPs) are a set of enzymes that participate in bacterial peptidoglycan assembly. The absolute numbers of each PBP were determined by direct measurement and have been reported for two Staphylococcus aureus strains, RN4220 (methicillin-sensitive S. aureus) and RN450M (methicillin-resista...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.2.588-591.2002
更新日期:2002-01-01 00:00:00
abstract::Helicobacter pylori is a microaerophilic bacterium associated with gastric inflammation and peptic ulcers. Knowledge of how pathogenic organisms produce energy is important from a therapeutic point of view. We found d-amino acid dehydrogenase-mediated electron transport from d-proline or d-alanine to oxygen via the re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01111-09
更新日期:2010-03-01 00:00:00
abstract::Azotobacter vinelandii DJ71, which contains a mutation in the nifV gene, was derepressed for nitrogenase in the presence of homocitrate. When dinitrogenase was isolated from this culture, it was found to be identical to the wild-type dinitrogenase. However, when the same NifV- strain was derepressed in the presence of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.17.5403-5405.1991
更新日期:1991-09-01 00:00:00
abstract::The generalized transducing phage T4GT7 grew well on recA strains of Escherichia coli and transduced recA into F- and Hfr strains of E. coli at high frequency. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.3.1072-1073.1984
更新日期:1984-09-01 00:00:00
abstract::Six uvr mutants of Saccharomyces cerevisiae with hypersensitivity to ultraviolet (UV) light were isolated after mutagen treatment with ethylmethanesulfonate. UV sensitivity ranges from moderate to extreme, and four of the mutants are also sensitive to nitrous acid. Ranking in terms of UV sensitivity does not parallel ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.3.571-575.1967
更新日期:1967-09-01 00:00:00
abstract::A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6732-6741.1991
更新日期:1991-11-01 00:00:00
abstract::The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00891-06
更新日期:2007-02-01 00:00:00
abstract::SwmB is required for swimming motility in Synechococcus sp. strain WH8102. This highly repetitive 1.12-MDa polypeptide is associated with the cell surface, where it is arranged in a punctate manner. Inactivation of swmB does not affect the localization of SwmA, an S-layer protein also required for swimming. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01500-06
更新日期:2007-02-01 00:00:00
abstract::The activities of DNA methyltransferases are important for a variety of cellular functions in bacteria. In this study, we developed a modified high-throughput technique called methyl homopolymer tail mediated sequencing (methyl HTM-seq) to identify the undermethylated sites in the Vibrio cholerae genome for the two DN...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02112-12
更新日期:2013-05-01 00:00:00
abstract::Vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. Motility is thought to be an important factor for the virulence of V. cholerae. The genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.6.1927-1930.1999
更新日期:1999-03-01 00:00:00
abstract::Mutants lacking an enzyme of the oxidative branch of the hexose monophosphate shunt, 6-phosphogluconolactonase (pgl), have been selected as a new class of glucose-negative derivatives of a phosphoglucose isomerase (pgi) mutant. Glucose negativity is not as complete as in mutants lacking phosphoglucose isomerase and gl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1296-1301.1969
更新日期:1969-12-01 00:00:00
abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02474-14
更新日期:2015-04-01 00:00:00
abstract::Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00336-17
更新日期:2017-12-20 00:00:00
abstract::The diphtheria toxin repressor (DtxR) uses Fe(2+) as a corepressor and inhibits transcription from iron-regulated promoters (IRPs) in Corynebacterium diphtheriae. A new IRP, designated IRP6, was cloned from C. diphtheriae by a SELEX-like procedure. DtxR bound to IRP6 in vitro only in the presence of appropriate divale...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.17.4846-4856.2002
更新日期:2002-09-01 00:00:00
abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3285-3292.1996
更新日期:1996-06-01 00:00:00