Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

abstract：:Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies reveal that the plasmids coding for the production of heat stable and heat labile enteroxtoxins of Escherichia coli, regardless of their origin, have a majority of their polynucleotide sequences in common, but are not related in any significant way to t...

journal_title：Journal of bacteriology

authors： So M,Crosa JH,Falkow S

更新日期：1975-01-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli O157:H7 (EHEC) causes bloody diarrhea and hemolytic-uremic syndrome. EHEC encodes the sRNA chaperone Hfq, which is important in posttranscriptional regulation. In EHEC strain EDL933, Hfq acts as a negative regulator of the locus of enterocyte effacement (LEE), which encodes most of t...

journal_title：Journal of bacteriology

authors： Kendall MM,Gruber CC,Rasko DA,Hughes DT,Sperandio V

更新日期：2011-12-01 00:00:00

abstract：:Escherichia coli recF mutants are hypermutable when treated with methyl methanesulfonate (G. C. Walker, Mol. Gen. Genet. 152:93-103, 1977). In this study, methylation hypermutability of recF mutant strains was examined, and it was found that recF+ is required for normal induction of the adaptive response to alkylation...

journal_title：Journal of bacteriology

authors： Volkert MR

更新日期：1989-01-01 00:00:00

abstract：:The protein SpoIVB plays a key role in signaling in the final sigma(K) checkpoint of Bacillus subtilis. This regulatory mechanism coordinates late gene expression during development in this organism and we have recently shown SpoIVB to be a serine peptidase. SpoIVB signals by transiting a membrane, undergoing self-cle...

journal_title：Journal of bacteriology

authors： Hoa NT,Brannigan JA,Cutting SM

更新日期：2002-01-01 00:00:00

abstract：:A DNA sequence (brpA) which regulates the expression of the genes of the bialaphos biosynthesis pathway (bap) in Streptomyces hygroscopicus was identified and characterized. A newly isolated nonproducing mutant (NP57) had a pleiotropic defect involving at least 6 of the 13 known bap genes; only the step 6 conversion c...

journal_title：Journal of bacteriology

authors： Anzai H,Murakami T,Imai S,Satoh A,Nagaoka K,Thompson CJ

更新日期：1987-08-01 00:00:00

abstract：:Fourteen bacterial strains of marine origin, which formed colonies containing crystals of a blue-black pigment, were submitted to a morphological, physiological, and nutritional characterization. The results indicated that these organisms form a new species of the genus Beneckea. ...

journal_title：Journal of bacteriology

authors： Baumann P,Baumann L,Mandel M,Allen RD

更新日期：1971-12-01 00:00:00

abstract：:Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB...

journal_title：Journal of bacteriology

authors： Willetts NS,Clark AJ

更新日期：1969-10-01 00:00:00

abstract：:Teichoic acid-like material extracted by cold trichloroacetic acid from lyophilized whole cells of streptococci from groups A,D,E,O, and T was shown to give a positive precipitin reaction with group antisera. Similar material from cells of groups B,C,F,G,H,K,L,M,N,P,Q,R, and S did not give a positive reaction with gro...

journal_title：Journal of bacteriology

authors： Matsuno T,Slade HD

更新日期：1970-06-01 00:00:00

abstract：:Bacterial biofilms are important in natural settings, biotechnology, and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread...

journal_title：Journal of bacteriology

authors： Bartolini M,Cogliati S,Vileta D,Bauman C,Rateni L,Leñini C,Argañaraz F,Francisco M,Villalba JM,Steil L,Völker U,Grau R

更新日期：2018-12-20 00:00:00

abstract：:Commonly used procedures effected the extraction of only 10% of the lipoteichoic acid of stationary-phase cells of Staphylococcus aureus and Streptococcus faecium, unless the cells were first disrupted. ...

journal_title：Journal of bacteriology

authors： Huff E

更新日期：1982-01-01 00:00:00

abstract：:In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...

journal_title：Journal of bacteriology

authors： Hall JA,Pajor AM

更新日期：2007-02-01 00:00:00

abstract：:Previously, the effects of three point mutations (at amino acid positions 31, 84, and 194) in the gene coding for a sulfonamide-resistant dihydropteroate synthase of Neisseria meningitidis were analyzed by site-directed mutagenesis. Changes at positions 31 and 194 abolished the phenotypic expression of sulfonamide res...

journal_title：Journal of bacteriology

authors： Fermér C,Swedberg G

更新日期：1997-02-01 00:00:00

abstract：:The fine structure of Paracoccidioides brasiliensis undergoing temperature-dependent transformation from mycelium to yeast and vice versa (M right harpoon over left harpoon Y) was studied. The transitional form to mycelium from the yeast appears as an elongated bud that extends from the yeast and which has a mixture o...

journal_title：Journal of bacteriology

authors： Carbonell LM

更新日期：1969-11-01 00:00:00

abstract：:Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

journal_title：Journal of bacteriology

authors： Iqbal J,Qureshi SA

更新日期：2010-06-01 00:00:00

abstract：:The O antigen of the Shigella flexneri lipopolysaccharide (LPS) is an important virulence determinant and immunogen. We have isolated S. flexneri mutants which produce a semi-rough LPS by using an O-antigen-specific phage, Sf6c. Western immunoblotting was used to show that the LPS produced by the semi-rough mutants co...

journal_title：Journal of bacteriology

authors： Morona R,Mavris M,Fallarino A,Manning PA

更新日期：1994-02-01 00:00:00

abstract：:We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...

journal_title：Journal of bacteriology

authors： Ericsson M,Hanstorp D,Hagberg P,Enger J,Nyström T

更新日期：2000-10-01 00:00:00

abstract：:We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

journal_title：Journal of bacteriology

authors： Perez-Casal JF,Gammie AE,Crosa JH

更新日期：1989-04-01 00:00:00

abstract：:Sulfolobus sulfataricus ATCC 35091, Haloferax volcanii, and Methanosarcina thermophila TM-1, representing the Euryarchaeota and Crenarchaeota subdomains of the Archaea, contain proteins which are phosphorylated on tyrosine. These data raise fundamental questions as to the origin and evolution of tyrosine phosphorylati...

journal_title：Journal of bacteriology

authors： Smith SC,Kennelly PJ,Potts M

更新日期：1997-04-01 00:00:00

abstract：:Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...

journal_title：Journal of bacteriology

authors： Hsu CC,Chen CW

更新日期：2010-01-01 00:00:00

abstract：:Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....

journal_title：Journal of bacteriology

authors： NAKATA HM

更新日期：1963-09-01 00:00:00

abstract：:A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

journal_title：Journal of bacteriology

authors： Lee JH,Wendt JC,Shanmugam KT

更新日期：1990-04-01 00:00:00

abstract：:The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl ...

journal_title：Journal of bacteriology

authors： Maier E,Polleichtner G,Boeck B,Schinzel R,Benz R

更新日期：2001-01-01 00:00:00

abstract：:A cell-free system from Bacillus thuringiensis was found to actively incorporate phenylalanine into hot trichloroacetic acid-precipitable material in the presence of synthetic polynucleotide, ribosomes, S-100 supernatant, an energy-generating system, and guanosine triphosphate. Phenylalanine incorporation was absolute...

journal_title：Journal of bacteriology

authors： Changchien LM,Aronson JN

更新日期：1970-09-01 00:00:00

abstract：:Rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. An effective live attenuated vaccine would be extremely useful in the prevention of R. equi disease in horses. Toward that end, we have developed an efficient transposon mu...

journal_title：Journal of bacteriology

authors： Ashour J,Hondalus MK

更新日期：2003-04-01 00:00:00

abstract：:Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...

journal_title：Journal of bacteriology

authors： Gardner SP,Olson JW

更新日期：2018-10-10 00:00:00

abstract：:Extracytoplasmic function σ factors that are stress inducible are often sequestered in an inactive complex with a membrane-associated anti-σ factor. Mycobacterium tuberculosis membrane-associated anti-σ factors have a small, stable RNA gene A (ssrA)-like degron for targeted proteolysis. Interaction between the unfolda...

journal_title：Journal of bacteriology

authors： Joshi AC,Kaur P,Nair RK,Lele DS,Nandicoori VK,Gopal B

更新日期：2019-02-25 00:00:00

abstract：:Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5...

journal_title：Journal of bacteriology

authors： Jankowitsch F,Schwarz J,Rückert C,Gust B,Szczepanowski R,Blom J,Pelzer S,Kalinowski J,Mack M

更新日期：2012-12-01 00:00:00

abstract：:Nocardia brasiliensis possess proteolytic activities that can be readily detected in a variety of media. In a modified formulation of a growth medium originally used for Streptomyces aureofaciens, N. brasiliensis was found to secrete proteolytic enzymes, one of which was capable of hydrolyzing casein. This enzyme was ...

journal_title：Journal of bacteriology

authors： Zlotnik H,Schramm VL,Buckley HR

更新日期：1984-02-01 00:00:00

abstract：:A mutant of Mycobacterium smegmatis unable to use the dipeptide carnosine (beta-alanyl-L-histidine) as a sole carbon or nitrogen source was isolated. Carnosinase activity and the ability to grow on beta-Ala and/or L-His were similar in the mutant and the wild type. However, the mutant showed significant impairment in ...

journal_title：Journal of bacteriology

authors： Bhatt A,Green R,Coles R,Condon M,Connell ND

更新日期：1998-12-01 00:00:00

abstract：:Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...

journal_title：Journal of bacteriology

authors： Du W,Brown JR,Sylvester DR,Huang J,Chalker AF,So CY,Holmes DJ,Payne DJ,Wallis NG

更新日期：2000-08-01 00:00:00