Characteristics of some multiply recombination-deficient strains of Escherichia coli.

Abstract:

:Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13. All multiple rec(-) strains carrying recA13 were similar to AB2463, which carries recA13 alone, in their UV sensitivities, recombination deficiencies, and inabilities to induce lambda phage in a lysogen. However, whereas AB2463 shows a high rate of ultraviolet (UV)-induced deoxyribonucleic acid (DNA) breakdown, the multiple rec(-) strains showed the low level characteristic of strains carrying recC22 or recB21 alone. The strain carrying both recC22 and recB21 was similar in all properties to the single mutants, suggesting that both gene products act in the same part of the recombination and UV repair pathways. It is concluded that in a Rec(+) strain, the recA(+) product acts to inhibit DNA breakdown determined by the recC(+) and recB(+) products.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Willetts NS,Clark AJ

doi

10.1128/JB.100.1.231-239.1969

subject

Has Abstract

pub_date

1969-10-01 00:00:00

pages

231-9

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

100

pub_type

杂志文章
  • Inhibitio of flagellar coordination in Spirillum volutans.

    abstract::The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.5.1431-1436.1967

    authors: Krieg NR,Tomelty JP,Wells JS Jr

    更新日期:1967-11-01 00:00:00

  • Regulation of derepressed synthesis of arylsulfatase by tyramine oxidase in Salmonella typhimurium.

    abstract::The participation of tyramine oxidase in the regulation of arylsulfatase synthesis in Salmonella typhimurium was studied. Arylsulfatase synthesis was repressed by inorganic sulfate, cysteine, methionine, or taurine. This repression was relieved by tyramine, octopamine, or dopamine, which induced tyramine oxidase synth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.796-802.1981

    authors: Murooka Y,Harada T

    更新日期:1981-02-01 00:00:00

  • Transport of glutamine by Streptococcus bovis and conversion of glutamine to pyroglutamic acid and ammonia.

    abstract::Streptococcus bovis JB1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a pH of 5.0 to 7.5; sodium had little effect on the transport rate. Because valinomycin-treated cells loaded with K and diluted into Na (pH 6.5) to create an artificial delta psi took up little gluta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.2981-2985.1989

    authors: Chen GJ,Russell JB

    更新日期:1989-06-01 00:00:00

  • Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.

    abstract::The secondary metabolite hydrogen cyanide (HCN) is produced by Pseudomonas fluorescens from glycine, essentially under microaerophilic conditions. The genetic basis of HCN synthesis in P. fluorescens CHA0 was investigated. The contiguous structural genes hcnABC encoding HCN synthase were expressed from the T7 promoter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.12.3187-3196.1998

    authors: Laville J,Blumer C,Von Schroetter C,Gaia V,Défago G,Keel C,Haas D

    更新日期:1998-06-01 00:00:00

  • Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

    abstract::Proline-requiring mutants of Saccharomyces cerevisiae were isolated. Each mutation is recessive and is inherited as expected for a single nuclear gene. Three complementation groups cold be defined which are believed to correspond to mutations in the three genes (pro1, pro2, and pro3) coding for the three enzymes of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.138.3.816-822.1979

    authors: Brandriss MC

    更新日期:1979-06-01 00:00:00

  • Mechanism of tuberculostasis in mammalian serum. II. Induction of serum tuberculostasis in guinea pigs.

    abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.64-70.1969

    authors: Kochan I,Golden CA,Bukovic JA

    更新日期:1969-10-01 00:00:00

  • Regulation and function of pilus island 1 in group B streptococcus.

    abstract::Group B streptococcus (GBS) pili may enhance colonization and infection by mediating bacterial adhesion to host cells, invasion across endothelial and epithelial barriers, and resistance to bacterial ingestion and killing by host phagocytes. However, it remains unclear how pilus expression is regulated and how modulat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00202-12

    authors: Jiang S,Park SE,Yadav P,Paoletti LC,Wessels MR

    更新日期:2012-05-01 00:00:00

  • Transport of vitamin B12 in Escherichia coli: common receptor system for vitamin B12 and bacteriophage BF23 on the outer membrane of the cell envelope.

    abstract::We showed previously that the outer membrane of the Escherichia coli cell envelope normally contains about 200 to 250 B12 receptors, and that these receptors function both in B12 transport and as receptors for the E colicins. This paper shows that this receptor system is also shared with bacteriophage BF23. A strong p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.1032-1039.1976

    authors: Bradbeer C,Woodrow ML,Khalifah LI

    更新日期:1976-03-01 00:00:00

  • CULTURE AND FLUORESCENT-ANTIBODY METHODS IN DIAGNOSIS OF WHOOPING COUGH.

    abstract::Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering. Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86:449-451. 1963.-Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.3.449-451.1963

    authors: HOLWERDA J,ELDERING G

    更新日期:1963-09-01 00:00:00

  • Myxococcus xanthus does not respond chemotactically to moderate concentration gradients.

    abstract::Using a number of approaches we were unable to demonstrate a chemotactic response of Myxococcus xanthus to a variety of defined and complex materials. These data in addition to a number of prima facie arguments considerably reduce the likelihood that M. xanthus possesses a mechanism for chemotactic behavior. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.437-442.1983

    authors: Dworkin M,Eide D

    更新日期:1983-04-01 00:00:00

  • A selenium-dependent xanthine dehydrogenase triggers biofilm proliferation in Enterococcus faecalis through oxidant production.

    abstract::Selenium has been shown to be present as a labile cofactor in a small class of molybdenum hydroxylase enzymes in several species of clostridia that specialize in the fermentation of purines and pyrimidines. This labile cofactor is poorly understood, yet recent bioinformatic studies have suggested that Enterococcus fae...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01063-10

    authors: Srivastava M,Mallard C,Barke T,Hancock LE,Self WT

    更新日期:2011-04-01 00:00:00

  • INTERACTION OF AN INTERFERON WITH L CELLS.

    abstract::Lockart, Royce Z., Jr. (The University of Texas, Austin) and Barbara Horn. Interaction of an interferon with L cells. J. Bacteriol. 85:996-1002. 1963.-Data were presented on the effect of time of exposure and concentration of an interferon in provoking viral inhibition in L cells. Populations of L cells which made red...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.5.996-1002.1963

    authors: LOCKART RZ Jr,HORN B

    更新日期:1963-05-01 00:00:00

  • Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus.

    abstract::The McrB restriction system of Escherichia coli K-12 is responsible for the biological inactivation of foreign DNA that contains 5-methylcytosine residues (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986). Within the McrB region of the chromosome is the mcrB gene, which encodes a protein of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.1974-1981.1989

    authors: Ross TK,Achberger EC,Braymer HD

    更新日期:1989-04-01 00:00:00

  • Propagation and growth cycle of Rickettsia quintana in a new liquid medium.

    abstract::The growth cycle of Rickettsia quintana was studied for the first time in liquid culture. Growth of the microorganism in a transparent broth medium was made possible by the finding that fetal calf serum (FCS), but not calf serum (CS), satisfied the requirement of R. quintana (Fuller strain) for red blood cell lysate. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.103.1.184-190.1970

    authors: Mason RA

    更新日期:1970-07-01 00:00:00

  • Identification of a methyl-specific restriction system mediated by a conjugative element from Streptomyces bambergiensis.

    abstract::pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.16.4809-4812.1995

    authors: Zotchev SB,Schrempf H,Hutchinson CR

    更新日期:1995-08-01 00:00:00

  • Identification of a novel nutrient-sensing histidine kinase/response regulator network in Escherichia coli.

    abstract::When carbon sources become limiting for growth, bacteria must choose which of the remaining nutrients should be used first. We have identified a nutrient-sensing signaling network in Escherichia coli that is activated at the transition to stationary phase. The network is composed of the two histidine kinase/response r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01554-14

    authors: Behr S,Fried L,Jung K

    更新日期:2014-06-01 00:00:00

  • Turnover of phosphatidylglycerol in Escherichia coli.

    abstract::In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a muta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.1.210-214.1973

    authors: Ballesta JP,De Garcia CL,Schaechter M

    更新日期:1973-10-01 00:00:00

  • Orientation of the guanine operon of Escherichia coli K-12 by utilizing strains containing guaB-xse and guaB-upp deletions.

    abstract::Temperature induction of an Escherichia coli strains with lambda cI1857 integrated in the guaB gene has been used to produce strains containing chromosomal deletions extending into the xse and upp genes. By utilizing strains containing these deletions, it has been possible to order the genes in the guanine operon with...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.1.320-322.1979

    authors: Vales LD,Chase JW,Murphy JB

    更新日期:1979-07-01 00:00:00

  • The Rhizobium etli cyaC product: characterization of a novel adenylate cyclase class.

    abstract::Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.13.3560-3568.2002

    authors: Téllez-Sosa J,Soberón N,Vega-Segura A,Torres-Márquez ME,Cevallos MA

    更新日期:2002-07-01 00:00:00

  • Assimilatory sulfur metabolism in marine microorganisms: characteristics and regulation of sulfate transport in Pseudomonas halodurans and Alteromonas luteo-violaceus.

    abstract::Sulfate transport capacity was not regulated by cysteine, methionine, or glutathione in Pseudomonas halodurans, but growth on sulfate or thiosulfate suppressed transport. Subsequent sulfur starvation of cultures grown on all sulfur sources except glutathione stimulated uptake. Only methionine failed to regulate sulfat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.2.340-349.1981

    authors: Cuhel RL,Taylor CD,Jannasch HW

    更新日期:1981-08-01 00:00:00

  • Control of arginine utilization in Neurospora.

    abstract::The response of Neurospora to changes in the availibility of exogenous arginine was investigated. Upon addition of arginine to the growth medium, catabolism is initiated within minutes. This occurs prior to expansion of the arginine pool or augmentation of catabolic enzyme levels. (Basal levels are approximately 25% o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.866-873.1977

    authors: Weiss RL,Davis RH

    更新日期:1977-02-01 00:00:00

  • Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones.

    abstract::Decreased intracellular levels of FtsH, a membrane-bound ATPase, led to retardation of growth and protein export, as well as to an abnormal translocation of alkaline phosphatase that had been attached to a cytoplasmic domain of a multispanning membrane protein, SecY. The last phenotype is designated Std (stop transfer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.4.1141-1145.1996

    authors: Shirai Y,Akiyama Y,Ito K

    更新日期:1996-02-01 00:00:00

  • Genetic and physical analyses of Caulobacter crescentus trp genes.

    abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.279-287.1984

    authors: Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

    更新日期:1984-10-01 00:00:00

  • Genome sequence of proteorhodopsin-containing sea ice bacterium Glaciecola punicea ACAM 611T.

    abstract::Here, we report the draft genome sequence of Antarctic sea ice bacterium Glaciecola punicea ACAM 611(T), the type species of the genus Glaciecola. A blue-light-absorbing proteorhodopsin gene is present in the 3.08-Mb genome. This genome sequence can facilitate the study of the physiological metabolisms and ecological ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00463-12

    authors: Qin QL,Xie BB,Shu YL,Rong JC,Zhao DL,Zhang XY,Chen XL,Zhou BC,Zhang YZ

    更新日期:2012-06-01 00:00:00

  • Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the beta subclass.

    abstract::Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.13.4172-4178.1997

    authors: Hurek T,Egener T,Reinhold-Hurek B

    更新日期:1997-07-01 00:00:00

  • Sequence and analysis of a plasmid-encoded mercury resistance operon from Mycobacterium marinum identifies MerH, a new mercuric ion transporter.

    abstract::In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01063-08

    authors: Schué M,Dover LG,Besra GS,Parkhill J,Brown NL

    更新日期:2009-01-01 00:00:00

  • Distribution of Tn551 insertion sites responsible for auxotrophy on the Staphylococcus aureus chromosome.

    abstract::A method was devised to efficiently select isolates of Staphylococcus aureus 8325 in which Tn551, a transposon originating on the pI258 plasmid responsible for erythromycin resistance (Emr), had translocated to the host chromosome. This method consisted of selecting for Emr at 43 degrees C with a strain in which the p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.1.479-488.1981

    authors: Pattee PA

    更新日期:1981-01-01 00:00:00

  • Potassium permeability of Rickettsia prowazekii.

    abstract::The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.1.197-201.1984

    authors: Winkler HH

    更新日期:1984-01-01 00:00:00

  • Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome.

    abstract::Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.479-489.1979

    authors: Linn T,Goman M,Scaife J

    更新日期:1979-11-01 00:00:00

  • Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

    abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.125-135.1976

    authors: Guild WR,Shoemaker NB

    更新日期:1976-01-01 00:00:00