Isolation and preliminary characterization of Saccharomyces cerevisiae proline auxotrophs.

abstract：:Kobayashi, George S. (Tulane University, New Orleans, La.), Lorraine Friedman, and Judith F. Kofroth. Some cytological and pathogenic properties of spheroplasts of Candida albicans. J. Bacteriol. 88:795-801. 1964.-Spheroplasts of Candida albicans were prepared by use of an enzymatic mixture from the digestive tract of...

journal_title：Journal of bacteriology

authors： KOBAYASHI GS,FRIEDMAN L,KOFROTH JF

更新日期：1964-09-01 00:00:00

abstract：:In a glycerol auxotroph of Staphylococcus aureus, the deprivation of glycerol affected the formation of certain membrane components. (i) There was synthesis of fatty acids at the predeprivation rate even though the fatty acids synthesized accumulated as free fatty acids rather than as esterified fatty acids; (ii) ther...

journal_title：Journal of bacteriology

authors： Ray PH,Lillich TT,White DC

更新日期：1972-10-01 00:00:00

abstract：:A group of enzymes of sulfur metabolism (arylsulfatase, cholinesulfatase, and a number of others) are normally repressed in Neurospora crassa by an abundant supply of a "favored" sulfur source such as methionine or inorganic sulfate. A mutant called scon(c) was isolated in which the formation of each of these enzymes ...

journal_title：Journal of bacteriology

authors： Burton EG,Metzenberg RL

更新日期：1972-01-01 00:00:00

abstract：:A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates. The gene was expressed in Escherichia coli from its own promoter. The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 7...

journal_title：Journal of bacteriology

authors： Rumbak E,Rawlings DE,Lindsey GG,Woods DR

更新日期：1991-11-01 00:00:00

abstract：:Con minus mutants, conjugation-defective mutants of Escherichia coli K-12, have been shown to be tolerant to colicins K and L. They map at approximately 14 min on the genetic map. The significance of the colicin tolerance of conjugation-defective mutants is discussed. ...

journal_title：Journal of bacteriology

authors： Davies JK,Reeves P

更新日期：1975-07-01 00:00:00

abstract：:Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

journal_title：Journal of bacteriology

authors： Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

更新日期：2008-05-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

journal_title：Journal of bacteriology

authors： Ottemann KM,DiRita VJ,Mekalanos JJ

更新日期：1992-11-01 00:00:00

abstract：:The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...

journal_title：Journal of bacteriology

authors： Jackson DW,Suzuki K,Oakford L,Simecka JW,Hart ME,Romeo T

更新日期：2002-01-01 00:00:00

abstract：:The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were...

journal_title：Journal of bacteriology

authors： Shukla HD,DasSarma S

更新日期：2004-05-01 00:00:00

abstract：:The effects of surfaces on the physiology of bacteria adhering to surfaces or immobilized within biofilms are receiving more interest. A study of the effects of hydrophobic and hydrophilic substrata on the colonization behavior of a marine bacterium, SW5, revealed major differences in the morphology of SW5 on these su...

journal_title：Journal of bacteriology

authors： Dalton HM,Poulsen LK,Halasz P,Angles ML,Goodman AE,Marshall KC

更新日期：1994-11-01 00:00:00

abstract：:Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...

journal_title：Journal of bacteriology

authors： Oertel W,Goulian M

更新日期：1979-11-01 00:00:00

abstract：:Two isoleucine analogues and two leucine analogues were examined for their ability to replace the natural amino acid preventing the accumulation of threonine deaminase-forming potential. The procedure used to study repression by the analogues distinguishes between true repression and the formation of inactive enzyme b...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1973-11-01 00:00:00

abstract：:The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents. Here, we provide evidence that oxidative lethal lesions are repaired by both endonuclease III and endonuclease VIII and that...

journal_title：Journal of bacteriology

authors： Saito Y,Uraki F,Nakajima S,Asaeda A,Ono K,Kubo K,Yamamoto K

更新日期：1997-06-01 00:00:00

abstract：:An 846-bp cryptic plasmid has been discovered in the hyperthermophilic bacterium Thermotoga sp. strain RQ7. This is the first plasmid described for an organism from this ancient bacterial lineage and the smallest plasmid described to date for any organism. Nucleotide sequencing revealed a single open reading frame pos...

journal_title：Journal of bacteriology

authors： Harriott OT,Huber R,Stetter KO,Betts PW,Noll KM

更新日期：1994-05-01 00:00:00

abstract：:Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...

journal_title：Journal of bacteriology

authors： Hsu CC,Chen CW

更新日期：2010-01-01 00:00:00

abstract：:Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...

journal_title：Journal of bacteriology

authors： Chandra TS,Friedrich CG

更新日期：1986-05-01 00:00:00

abstract：:In Escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein MotA and the rotor protein FliG. These charged residues are conserved in the Na+-driven polar flagellum of Vibrio alginolyticus, but mutational studies in V. algi...

journal_title：Journal of bacteriology

authors： Yakushi T,Yang J,Fukuoka H,Homma M,Blair DF

更新日期：2006-02-01 00:00:00

abstract：:Schwartz, Norman M. (Yale University, New Haven, Conn.). Genetic instability in Escherichia coli. J. Bacteriol. 89:712-717. 1965.-Results obtained from a transductional analysis demonstrated that an extremely unstable reversion of lac(-) is a suppressor mutation. This mutation suppresses 2 of 22 different lac(-) allel...

journal_title：Journal of bacteriology

authors： SCHWARTZ NM

更新日期：1965-03-01 00:00:00

abstract：:Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a ...

journal_title：Journal of bacteriology

authors： Sawers G,Hesslinger C,Muller N,Kaiser M

更新日期：1998-07-01 00:00:00

abstract：:A protein carboxyl methyltransferase (EC 2.1.1.77) that recognizes age-damaged proteins for potential repair or degradation reactions has been found in all vertebrate tissues and cells examined to date. This enzyme catalyzes the transfer of methyl groups from S-adenosylmethionine to the carboxyl groups of D-aspartyl o...

journal_title：Journal of bacteriology

authors： Li C,Clarke S

更新日期：1992-01-01 00:00:00

abstract：:A number of laboratories are now engaged in the genetic analysis of Candida albicans. This diploid yeast, the major fungal pathogen of humans, is imperfect. Parasexual techniques have been devised for complementation and recombination analysis in this organism. This paper attempts to address the question of the extent...

journal_title：Journal of bacteriology

authors： Poulter R,Hanrahan V

更新日期：1983-11-01 00:00:00

abstract：:Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

journal_title：Journal of bacteriology

authors： Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

更新日期：2005-03-01 00:00:00

abstract：:The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

journal_title：Journal of bacteriology

authors： Kim KS,Chilton WS,Farrand SK

更新日期：1996-06-01 00:00:00

abstract：:The bacteriophage T4 MotA protein is a transcriptional activator of T4-modified host RNA polymerase and is required for activation of the middle class of T4 promoters. MotA alone binds to the -30 region of T4 middle promoters, a region that contains the MotA box consensus sequence [(t/a)(t/a)TGCTT(t/c)A]. We report th...

journal_title：Journal of bacteriology

authors： Gerber JS,Hinton DM

更新日期：1996-11-01 00:00:00

abstract：:Woody-Karrer, Pearl (Palo Alto Medical Research Foundation, Palo Alto, Calif.) and Joseph Greenberg. Resistance and cross resistance of Escherichia coli S mutants to the radiomimetic agent nitrofurazone. J. Bacteriol. 85:1208-1216. 1963.-Cross-resistance relationships are described for 73 mutants of Escherichia coli s...

journal_title：Journal of bacteriology

authors： WOODY-KARRER P,GREENBERG J

更新日期：1963-06-01 00:00:00

abstract：:An alcohol dehydrogenase (ADH) from hyperthermophilic archaeon Thermococcus guaymasensis was purified to homogeneity and was found to be a homotetramer with a subunit size of 40 ± 1 kDa. The gene encoding the enzyme was cloned and sequenced; this gene had 1,095 bp, corresponding to 365 amino acids, and showed high seq...

journal_title：Journal of bacteriology

authors： Ying X,Ma K

更新日期：2011-06-01 00:00:00

abstract：:Xylanase activity of Clostridium cellulovorans, an anaerobic, mesophilic, cellulolytic bacterium, was characterized. Most of the activity was secreted into the growth medium when the bacterium was grown on xylan. Furthermore, when the extracellular material was separated into cellulosomal and noncellulosomal fractions...

journal_title：Journal of bacteriology

authors： Kosugi A,Murashima K,Doi RH

更新日期：2001-12-01 00:00:00

abstract：:ruvC mutants of Escherichia coli appear to lack an activity that resolves Holliday intermediates into recombinant products. Yet, these strains produce close to normal numbers of recombinants in genetic crosses. This recombination proficiency was found to be a function of recG. A "mini-kan" insertion in recG was introd...

journal_title：Journal of bacteriology

authors： Lloyd RG

更新日期：1991-09-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil-dwelling alphaproteobacterium that engages in a nitrogen-fixing root nodule symbiosis with leguminous plants. Cell surface polysaccharides are important both for adapting to stresses in the soil and for the development of an effective symbiotic interaction. Among the polysaccharides ch...

journal_title：Journal of bacteriology

authors： Barnett MJ,Long SR

更新日期：2018-01-10 00:00:00