Genetic and physical analyses of Caulobacter crescentus trp genes.

abstract：:Sigma 54-dependent transcriptional activators such as Escherichia coli NtrC, Rhizobium meliloti NifA, and Rhizobium leguminosarum DctD share similar central and carboxy-terminal domains but differ in the structure and function of their amino-terminal domains. We have deleted the amino-terminal and carboxy-terminal dom...

journal_title：Journal of bacteriology

authors： Huala E,Stigter J,Ausubel FM

更新日期：1992-02-01 00:00:00

abstract：:Using transposon Tn5-mediated mutagenesis, an essential Rhizobium meliloti nitrogen fixation (nif) gene was identified and located directly downstream of the regulatory gene nifA. Maxicell and DNA sequence analysis demonstrated that the new gene is transcribed in the same direction as nifA and codes for a 54-kilodalto...

journal_title：Journal of bacteriology

authors： Buikema WJ,Klingensmith JA,Gibbons SL,Ausubel FM

更新日期：1987-03-01 00:00:00

abstract：:Wild-type cells of Aspergillus nidulans synthesize a transport system which appears to be specific for l-glutamate and l-aspartate. The system is energy dependent and concentrates l-glutamate at least 60-fold. In cells grown in the presence of 1% sucrose, l-glutamate uptake activity is regulated by ammonium control, a...

journal_title：Journal of bacteriology

authors： Pateman JA,Kinghorn JR,Dunn E

更新日期：1974-08-01 00:00:00

abstract：:Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...

journal_title：Journal of bacteriology

authors： SMITH DC,FOLTZ VD,LORD TH

更新日期：1964-12-01 00:00:00

abstract：:The mobilizable Bacteroides element NBU2 (11 kbp) was found originally in two Bacteroides clinical isolates, Bacteroides fragilis ERL and B. thetaiotaomicron DOT. At first, NBU2 appeared to be very similar to another mobilizable Bacteroides element, NBU1, in a 2.5-kbp internal region, but further examination of the fu...

journal_title：Journal of bacteriology

authors： Wang J,Shoemaker NB,Wang GR,Salyers AA

更新日期：2000-06-01 00:00:00

abstract：:Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

journal_title：Journal of bacteriology

authors： Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

更新日期：2000-07-01 00:00:00

abstract：:Narkates, Annie J. (Southern Research Institute, Birmingham, Ala.), and Robert F. Pittillo. Influence of inoculum media on sensitivity of Escherichia coli to azaserine. J. Bacteriol. 90:710-714. 1965.-Escherichia coli grown in a simple synthetic medium is more than 100-fold more sensitive to inhibition by azaserine th...

journal_title：Journal of bacteriology

authors： Narkates AJ,Pittillo RF

更新日期：1965-09-01 00:00:00

abstract：UNLABELLED:Many bacteria utilize actin-like proteins to direct peptidoglycan (PG) synthesis. MreB and MreB-like proteins are thought to act as scaffolds, guiding the localization and activity of key PG-synthesizing proteins during cell elongation. Despite their critical role in viability and cell shape maintenance, ver...

journal_title：Journal of bacteriology

authors： Duan Y,Sperber AM,Herman JK

更新日期：2016-07-13 00:00:00

abstract：:Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA. DNA sequencing revealed two long open reading frames upstream of and on the same strand as...

journal_title：Journal of bacteriology

authors： Watson N,Dunyak DS,Rosey EL,Slonczewski JL,Olson ER

更新日期：1992-01-01 00:00:00

abstract：:The antibiotic cerulenin causes the immediate cessation of phospholipid biosynthesis in both chemoheterotrophic and photoheterotrophic cultures of Rhodopseudomonas sphaeroides. Macromolecule biosynthesis in photoheterotrophic cells was unaffected by cerulenin for the first 2 h after antibiotic addition and then contin...

journal_title：Journal of bacteriology

authors： Shepherd WD,Kaplan S

更新日期：1983-12-01 00:00:00

abstract：:The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

journal_title：Journal of bacteriology

authors： Moralejo P,Egan SM,Hidalgo E,Aguilar J

更新日期：1993-09-01 00:00:00

abstract：:It has been previously established that Thiobacillus neapolitanus fixes CO2 by using a form I ribulose bisphosphate carboxylase/oxygenase (RuBisCO), that much of the enzyme is sequestered into carboxysomes, and that the genes for the enzyme, cbbL and cbbS, are part of a putative carboxysome operon. In the present stud...

journal_title：Journal of bacteriology

authors： Baker SH,Jin S,Aldrich HC,Howard GT,Shively JM

更新日期：1998-08-01 00:00:00

abstract：:Bacteria often encounter environments where nutrient availability is limited, and they must adapt accordingly. To identify Pasteurella multocida genes that are differentially expressed during nutrient limitation, we utilized whole-genome microarrays to compare levels of gene expression during growth in rich and minima...

journal_title：Journal of bacteriology

authors： Paustian ML,May BJ,Kapur V

更新日期：2002-07-01 00:00:00

abstract：:Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography. Upon incubation in the presence of radioactive ATP,...

journal_title：Journal of bacteriology

authors： Vincent C,Doublet P,Grangeasse C,Vaganay E,Cozzone AJ,Duclos B

更新日期：1999-06-01 00:00:00

abstract：:The cell walls of a selected isolate of Staphylococcus aureus FDA 209P were observed undergoing progressive disintegration when exposed to lysostaphin (1 unit/ml) in 24% NaCl solution. Electron micrographs of ultrathin sections of test cells after exposure to lysostaphin for 2 min showed only superficial evidence of l...

journal_title：Journal of bacteriology

authors： Schuhardt VT,Huber TW,Pope LM

更新日期：1969-01-01 00:00:00

abstract：:A series of independent Escherichia coli K eductants has been isolated and tested to determine the extent of their deletions. The deletions cover the P2 prophage in location H, the his operon, a suppressor of the recBC phenotype (sbcB), the gene for gluconate-6-phosphate dehydrogenase (gnd), a locus involved in cell w...

journal_title：Journal of bacteriology

authors： Sunshine MG,Kelly B

更新日期：1971-11-01 00:00:00

abstract：:Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

journal_title：Journal of bacteriology

authors： Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

更新日期：1995-11-01 00:00:00

abstract：:We investigated the capacity of the hyperthermophile Pyrococcus furiosus for DNA repair by measuring survival at high levels of 60Co gamma-irradiation. The P. furiosus 2-Mb chromosome was fragmented into pieces ranging from 500 kb to shorter than 30 kb at a dose of 2,500 Gy and was fully restored upon incubation at 95...

journal_title：Journal of bacteriology

authors： DiRuggiero J,Santangelo N,Nackerdien Z,Ravel J,Robb FT

更新日期：1997-07-01 00:00:00

abstract：:Spores of Bacillus subtilis have a thick outer layer of relatively insoluble protein called the coat, which protects spores against a number of treatments and may also play roles in spore germination. However, elucidation of precise roles of the coat in spore properties has been hampered by the inability to prepare sp...

journal_title：Journal of bacteriology

authors： Ghosh S,Setlow B,Wahome PG,Cowan AE,Plomp M,Malkin AJ,Setlow P

更新日期：2008-10-01 00:00:00

abstract：:The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...

journal_title：Journal of bacteriology

authors： Zhu H,Paradis FW,Krell PJ,Phillips JP,Forsberg CW

更新日期：1994-07-01 00:00:00

abstract：:Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen. It has caused a very large economic loss in the swine industry of China and has become a threat to human health. We announce the complete genome sequence of S. equi subsp. zooepidemicus strain ATCC 35246, which provides opportunities to understand i...

journal_title：Journal of bacteriology

authors： Ma Z,Geng J,Zhang H,Yu H,Yi L,Lei M,Lu CP,Fan HJ,Hu S

更新日期：2011-10-01 00:00:00

abstract：:In Salmonella, ilv-linked rfe genes participate in the biosynthesis of the enterobacterial common antigen (CA) as well as of certain types of O antigen (serogroups C1 and L). rff genes, probably in the same cluster with rfe, are required for CA synthesis (P.H. Mäkelä et al., in preparation). Several Escherichia coli s...

journal_title：Journal of bacteriology

authors： Schmidt G,Mayer H,Mäkelä PH

更新日期：1976-08-01 00:00:00

abstract：:Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...

journal_title：Journal of bacteriology

authors： Rottem S,Markowitz O

更新日期：1979-12-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Acetyl coenzyme A synthetase (Acs) activates acetate to acetyl coenzyme A through an acetyladenylate intermediate; two other enzymes, acetate kinase (Ack) and phosphotransacetylase (Pta), activate acetate through an acetyl phosphate intermediate. We subcloned acs, the Escherichia coli open reading frame purported to e...

journal_title：Journal of bacteriology

authors： Kumari S,Tishel R,Eisenbach M,Wolfe AJ

更新日期：1995-05-01 00:00:00

abstract：:The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

journal_title：Journal of bacteriology

authors： Gray JE,Wallen JW,Calhoun DH

更新日期：1982-07-01 00:00:00

abstract：:In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...

journal_title：Journal of bacteriology

authors： Newell SL,Brill WJ

更新日期：1972-08-01 00:00:00

abstract：:Until the question of symmetry or asymmetry in the facilitated diffusion of monosaccharides by Saccharomyces cerevisiae is resolved, attempts to study the transport process cannot be based on assumptions of symmetry, such as equal concentrations at equilibrium or kinetic parameters that are equal in opposite direction...

journal_title：Journal of bacteriology

authors： Kalsow CM,Doyle RJ

更新日期：1973-02-01 00:00:00

abstract：:The activities of phosphofructokinase (PFK), fructose diphosphatase (FDP), nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP)-linked isocitrate dehydrogenases (IDHNAD, IDHNADP), two NAD-linked glutamate dehydrogenases (GDH1, GDH2), and isocitrate lyase were studied during the development of the two pheno...

journal_title：Journal of bacteriology

authors： Ingebretsen OC,Sanner T

更新日期：1976-06-01 00:00:00

abstract：:A 3,373-base-pair DNA segment from a clone fortuitously isolated from Saccharopolyspora erythraea by hybridization to an oligodeoxynucleotide probe was sequenced. Computer-assisted analysis of the nucleotide sequence reveals three closely linked Streptomyces open reading frames plus a fourth converging on the others. ...

journal_title：Journal of bacteriology

authors： Donadio S,Shafiee A,Hutchinson CR

更新日期：1990-01-01 00:00:00