Abstract:
:Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E. coli cya mutant. Further, overexpression of a malE::cyaC fusion protein allowed the detection of significant AC activity levels in cell extracts of an E. coli cya mutant. CyaC is unrelated to any known AC or to any other protein exhibiting a currently known function. Thus, CyaC represents the first member of a novel class of ACs (class VI). Hypothetical genes of unknown function similar to cyaC have been identified in the genomes of the related bacterial species Mesorhizobium loti, Sinorhizobium meliloti, and Agrobacterium tumefaciens. The cyaC gene is cotranscribed with a gene similar to ohr of Xanthomonas campestris and is expressed only in the presence of organic hydroperoxides. The physiological performance of an R. etli cyaC mutant was indistinguishable from that of the wild-type parent strain both under free-living conditions and during symbiosis.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Téllez-Sosa J,Soberón N,Vega-Segura A,Torres-Márquez ME,Cevallos MAdoi
10.1128/jb.184.13.3560-3568.2002keywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
3560-8issue
13eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::Payne, W. J. (University of Georgia, Athens), and R. L. Todd. Flavin-linked dehydrogenation of ether glycols by cell-free extracts of a soil bacterium. J. Bacteriol. 91:1533-1536. 1966.-Cell-free extracts of bacterium TEG-5 grown on tetraethylene glycol dehydrogenated a variety of ether glycols and nonylphenoxy and se...
journal_title:Journal of bacteriology
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doi:10.1128/JB.91.4.1533-1536.1966
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abstract::Holwerda, Jack (Michigan Department of Health, Grand Rapids) and Grace Eldering. Culture and fluorescent-antibody methods in diagnosis of whooping cough. J. Bacteriol. 86:449-451. 1963.-Nasopharyngeal swabs from 517 suspected whooping-cough patients were examined by culture and by fluorescent-antibody (FA) staining pr...
journal_title:Journal of bacteriology
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doi:10.1128/JB.86.3.449-451.1963
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doi:10.1128/JB.157.3.746-750.1984
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.553-555.1976
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journal_title:Journal of bacteriology
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doi:10.1128/JB.00545-16
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.3.1524-1526.1978
更新日期:1978-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.159.3.951-957.1984
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.13.4475-4481.1992
更新日期:1992-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4772-4779.1993
更新日期:1993-08-01 00:00:00
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更新日期:1981-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1988-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-12-01 00:00:00
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更新日期:2009-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.3.1289-1295.1973
更新日期:1973-03-01 00:00:00