Purification and characterization of a primary-secondary alcohol dehydrogenase from two strains of Clostridium beijerinckii.

abstract：:We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...

journal_title：Journal of bacteriology

authors： Simonsen CC,Chen EY,Levinson AD

更新日期：1983-09-01 00:00:00

abstract：:Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...

journal_title：Journal of bacteriology

authors： Walderich B,Ursinus-Wössner A,van Duin J,Höltje JV

更新日期：1988-11-01 00:00:00

abstract：:Exfoliative toxin type B is produced by bacteriophage group II strains of Staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. In addition to exfoliative toxin B, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. These phenotypes, as well as re...

journal_title：Journal of bacteriology

authors： Jackson MP,Iandolo JJ

更新日期：1986-05-01 00:00:00

abstract：:Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...

journal_title：Journal of bacteriology

authors： Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

更新日期：2010-08-01 00:00:00

abstract：:We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance of that region for translation efficiency and mRNA stability. Transcriptional fusions of 17 mutants to lacZ expressed th...

journal_title：Journal of bacteriology

authors： Flache P,Baumeister R,Hillen W

更新日期：1992-04-01 00:00:00

abstract：:Several Pseudomonas solanacearum strains which produced no detectable extracellular polysaccharide (EPS) in planta had been reported to remain highly virulent when tested at high inoculum concentrations (P. Xu, M. Iwata, S. Leong, and L. Sequeira, J. Bacteriol. 172:3946-3951, 1990; P. Xu, S. Leong, and L. Sequeira, J....

journal_title：Journal of bacteriology

authors： Kao CC,Barlow E,Sequeira L

更新日期：1992-02-01 00:00:00

abstract：:Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...

journal_title：Journal of bacteriology

authors： Karunakaran KP,Noguchi Y,Read TD,Cherkasov A,Kwee J,Shen C,Nelson CC,Brunham RC

更新日期：2003-03-01 00:00:00

abstract：:A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...

journal_title：Journal of bacteriology

authors： Dickinson ES,Sundaram TK

更新日期：1970-03-01 00:00:00

abstract：:Clostridium perfringens type B and D isolates produce epsilon-toxin, the third most potent clostridial toxin. The epsilon-toxin gene (etx) is plasmid borne in type D isolates, but etx genetics have been poorly studied in type B isolates. This study reports the first sequencing of any etx plasmid, i.e., pCP8533etx, fro...

journal_title：Journal of bacteriology

authors： Miyamoto K,Li J,Sayeed S,Akimoto S,McClane BA

更新日期：2008-11-01 00:00:00

abstract：:Phosphorylation of lipopolysaccharide (LPS) from a psychrotrophic bacterium, Pseudomonas syringae, from Antarctica was studied by using sucrose gradient-separated membrane fractions. The bacterium was found to possess an LPS kinase which could phosphorylate more LPS postsynthetically at higher temperatures. The phosph...

journal_title：Journal of bacteriology

authors： Ray MK,Kumar GS,Shivaji S

更新日期：1994-07-01 00:00:00

abstract：:Although competence normally develops only in glucose-minimal salts media, mecA and mecB mutations permit the expression of competence and of late competence genes in complex media as well (D. Dubnau and M. Roggiani, J. Bacteriol. 172:4048-4055, 1990). The expression of late competence genes is dependent on the produc...

journal_title：Journal of bacteriology

authors： Roggiani M,Hahn J,Dubnau D

更新日期：1990-07-01 00:00:00

abstract：:Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...

journal_title：Journal of bacteriology

authors： Patel RN,Hou CT,Felix A,Lillard MO

更新日期：1976-07-01 00:00:00

abstract：:The effects of various metabolic inhibitors on the motility of Spirochaeta aurantia were investigated. After 15 min in sodium arsenate buffer, 90% of cells remained motile even though adenosine triphosphate levels dropped from 5.6 to 0.1 nmol/mg (dry weight) of cells. After 70 min in sodium arsenate, 5% of cells were ...

journal_title：Journal of bacteriology

authors： Goulbourne EA Jr,Greenberg EP

更新日期：1980-09-01 00:00:00

abstract：:A lytic enzyme active against viable, intact staphylococci is released into culture fluids upon lysis of bacteriophage-infected Staphylococcus aureus PS53 cells. This enzyme, staphylococcal phage-associated lysin (PAL), was partially purified by ammonium sulfate precipitation and gel filtration through Sephadex G-200....

journal_title：Journal of bacteriology

authors： Sonstein SA,Hammel JM,Bondi A

更新日期：1971-08-01 00:00:00

abstract：:Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.) and William Shirley. Phagocytosis inhibition and reversal. I. Effect of glycolytic intermediates and nucleotides on particle uptake. J. Bacteriol. 86:259-265. 1963.-By microscopically monitoring phagocytosis and following the biochemical changes associated wi...

journal_title：Journal of bacteriology

authors： SBARRA AJ,SHIRLEY W

更新日期：1963-08-01 00:00:00

abstract：:The intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic AMP) were measured at various intervals during growth and morphogenesis in Arthrobacter crystallopoietes. Cyclic AMP levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. Immediately after inoculat...

journal_title：Journal of bacteriology

authors： Hamilton RW,Achberger EC,Kolenbrander PE

更新日期：1977-02-01 00:00:00

abstract：:Three gene libraries of Bordetella avium 197 DNA were prepared in Escherichia coli LE392 by using the cosmid vectors pCP13 and pYA2329, a derivative of pCP13 specifying spectinomycin resistance. The cosmid libraries were screened with convalescent-phase anti-B. avium turkey sera and polyclonal rabbit antisera against ...

journal_title：Journal of bacteriology

authors： Gentry-Weeks CR,Hultsch AL,Kelly SM,Keith JM,Curtiss R 3rd

更新日期：1992-12-01 00:00:00

abstract：:Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino...

journal_title：Journal of bacteriology

authors： Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJ

更新日期：1998-06-01 00:00:00

abstract：:Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...

journal_title：Journal of bacteriology

authors： Setlow JK,Brown DC,Boling ME,Mattingly A,Gordon MP

更新日期：1968-02-01 00:00:00

abstract：:Accumulation of K+ ions and glutamate plays a primary role in maintaining osmotic balance in Escherichia coli, as illustrated by the high concentrations of these ions present in cells growing in medium of high osmolality. We found that two gamma-glutamyl peptides and glutamine also accumulated during growth at high os...

journal_title：Journal of bacteriology

authors： McLaggan D,Logan TM,Lynn DG,Epstein W

更新日期：1990-07-01 00:00:00

abstract：:Synthetic analogs of enterochelin (enterobactin) were tested for their ability to support the growth of Escherichia coli K-12 under iron-limiting conditions. The cyclic compound MECAM [1,3,5-N.N'; N"-tris-(2,3-dihydroxybenzoyl)-triamino-methylbenzene] and its N-methyl derivative Me3MECAM promoted growth, whereas the 2...

journal_title：Journal of bacteriology

authors： Heidinger S,Braun V,Pecoraro VL,Raymond KN

更新日期：1983-01-01 00:00:00

abstract：:A novel of Escherichia coli endopeptidase was used for a selective partial hydrolysis of the peptide bridges which interlink the glycan chains in E. coli sacculi. The loosening of the murein network revealed, in the electron microscope, a preferential orientation of the glycan chains, more or less perpendicular to the...

journal_title：Journal of bacteriology

authors： Verwer RW,Nanninga N,Keck W,Schwarz U

更新日期：1978-11-01 00:00:00

abstract：:The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in rec...

journal_title：Journal of bacteriology

authors： Wang WB,Tessman ES

更新日期：1985-07-01 00:00:00

abstract：:Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...

journal_title：Journal of bacteriology

authors： Jackson AA,Gross MJ,Daniels EF,Hampton TH,Hammond JH,Vallet-Gely I,Dove SL,Stanton BA,Hogan DA

更新日期：2013-07-01 00:00:00

abstract：:We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...

journal_title：Journal of bacteriology

authors： Zuberi AR,Ying CW,Parker HM,Ordal GW

更新日期：1990-12-01 00:00:00

abstract：:An Escherichia coli strain carrying the recB21 and res-1 mutations showed an abnormally low level of colony-forming ability although it grew essentially normally in liquid medium. The recB21 res-1 strain showed little, if any, of the ultraviolet (UV)-induced deoxyribonucleic acid (DNA) breakdown characteristic of the ...

journal_title：Journal of bacteriology

authors： Kato T

更新日期：1972-12-01 00:00:00

abstract：:Englesberg, E. (University of Pittsburgh, Pittsburgh, Pa.), R L. Anderson, R. Weinberg, N. Lee, P. Hoffee, G. Huttenhauer, and H. Boyer. l-Arabinose-sensitive, l-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli. J. Bacteriol. 84:137-146. 1962-l-Arabinose-negative mutants of Escherichia coli B/r, ...

journal_title：Journal of bacteriology

authors： ENGLESBERG E,ANDERSON RL,WEINBERG R,LEE N,HOFFEE P,HUTTENHAUER G,BOYER H

更新日期：1962-07-01 00:00:00

abstract：:In this report we describe the functional expression of EmrE, a 110-amino-acid multidrug transporter from Escherichia coli, in the yeast Saccharomyces cerevisiae. To allow for phenotypic complementation, a mutant strain sensitive to a series of cationic lipophilic drugs was first identified. A hemagglutinin epitope-ta...

journal_title：Journal of bacteriology

authors： Yelin R,Rotem D,Schuldiner S

更新日期：1999-02-01 00:00:00

abstract：:Synthesis of the autoinducer signal involved in the cell density-dependent activation of Vibrio fischeri luminescence is directed by luxI. The autoinducer is N-(3-oxohexanoyl)homoserine lactone, and little is known about its synthesis. We have measured autoinducer synthesis by amino acid auxotrophs of Escherichia coli...

journal_title：Journal of bacteriology

authors： Hanzelka BL,Greenberg EP

更新日期：1996-09-01 00:00:00

abstract：:Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

journal_title：Journal of bacteriology

authors： Samhita L,Nanjundiah V,Varshney U

更新日期：2014-07-01 00:00:00