Involvement of gamma-glutamyl peptides in osmoadaptation of Escherichia coli.

Abstract:

:Accumulation of K+ ions and glutamate plays a primary role in maintaining osmotic balance in Escherichia coli, as illustrated by the high concentrations of these ions present in cells growing in medium of high osmolality. We found that two gamma-glutamyl peptides and glutamine also accumulated during growth at high osmolarity. In a mutant unable to make trehalose growing in 1.3 osM medium, glutathione, gamma-glutamylglutamine, and glutamine accumulated to levels of 73, 33, and 140 mumol/g of protein, respectively. In such cells, K+ was present at 1,450 mumol/g of protein, indicating that glutathione and gamma-glutamylglutamine accounted for less than 10% of the low-molecular-weight anions accumulated with K+. However, glutathione is needed for wild-type osmotolerance in this species. A mutant deficient in glutathione because of an insertion in the gshA gene was unable to grow above 1.4 osM, grew more slowly at intermediate osmolarities, and took longer to adapt to growth following osmotic upshock. The involvement of glutathione in osmoregulation was independent of the effect of glutathione on K+ retention.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

McLaggan D,Logan TM,Lynn DG,Epstein W

doi

10.1128/jb.172.7.3631-3636.1990

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

3631-6

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

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