Abstract:
:We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by monitoring the cleavage of the fluorogenic neuraminidase substrate 2'-(4-methylumbelliferyl)-alpha-D-N-acetyl-neuraminic acid. Positive clones (BG6, BG7, BC4, and BC8) contained the identical 2-kb BclI-BglII fragment and expressed neuraminidase efficiently and constitutively using its own promoter in the heterologous host. From the nucleotide sequence analysis, an open reading frame of 1,941 bp which encodes a polypeptide with an M(r) of 68,840 was detected. The deduced amino acid sequence has five Asp boxes, -Ser-X-Asp-X-Gly-X-Thr-Trp, showing great similarity to other bacterial and viral neuraminidases. We have also identified the catalytic domain by using truncated proteins produced in S. lividans.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Sakurada K,Ohta T,Hasegawa Mdoi
10.1128/jb.174.21.6896-6903.1992keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
6896-903issue
21eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::The growth phase-dependent change in sucrose density gradient centrifugation patterns of ribosomes was analyzed for both laboratory strains of Escherichia coli and natural isolates from the ECOR collection. All of the natural isolates examined formed 100S ribosome dimers in the stationary phase, and ribosome modulatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.10.2893-2899.2000
更新日期:2000-05-01 00:00:00
abstract::Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.638-641.1979
更新日期:1979-05-01 00:00:00
abstract::Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/jb.175.6.1555-1560.1993
更新日期:1993-03-01 00:00:00
abstract::Adler, H. E. (University of California, Davis), M. Shifrine, and Herrad Ortmayer. Mycoplasma inocuum sp. n., a saprophyte from chickens. J. Bacteriol. 82:239-240. 1961.-A new species of Mycoplasma was isolated from the infraorbital sinuses of chickens. The organism, a saprophyte, differs from all known mycoplasmas of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.2.239-240.1961
更新日期:1961-08-01 00:00:00
abstract::NADPH is an intermediate in the oxidation of organic compounds coupled to Fe(III) reduction in Geobacter species, but Fe(III) reduction with NADPH as the electron donor has not been studied in these organisms. Crude extracts of Geobacter sulfurreducens catalyzed the NADPH-dependent reduction of Fe(III)-nitrilotriaceti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.15.4468-4476.2001
更新日期:2001-08-01 00:00:00
abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01569-07
更新日期:2008-10-01 00:00:00
abstract::An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.608-617.1974
更新日期:1974-11-01 00:00:00
abstract::The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.1867-1871.1997
更新日期:1997-03-01 00:00:00
abstract::An in vitro system of cell envelopes from Salmonella typhimurium with functional flagella was used to determine the minimal requirements for flagellar rotation. Rotation in the absence of cytoplasmic constituents could be driven either by respiration or by an artificially imposed chemical gradient of protons. No speci...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.3.1208-1210.1984
更新日期:1984-06-01 00:00:00
abstract::The human bacterial pathogens Chlamydia spp. possess a genus-specific lipopolysaccharide as a major surface antigen, the structure of which has been determined by analytical chemistry as Kdop alpha 2-8-Kdop alpha 2-4-Kdop alpha 2-6GlcNp beta 1-6-GlcNol (Kdo, 3-deoxy-D-manno-2-octulosonic acid). Immunochemical studies ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.1862-1866.1991
更新日期:1991-03-01 00:00:00
abstract::The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.4758-4765.1990
更新日期:1990-09-01 00:00:00
abstract::Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.) and Guy P. Youmans. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J. Bacteriol. 84:708-715. 1962.-A substance, called mycosuppressin, was found in the lungs of guinea pigs and rabbits vaccinated with BCG or...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.708-715.1962
更新日期:1962-10-01 00:00:00
abstract::In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.854-860.1985
更新日期:1985-11-01 00:00:00
abstract::The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5758-5766.1990
更新日期:1990-10-01 00:00:00
abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.245-254.1991
更新日期:1991-01-01 00:00:00
abstract::Three homofermentative (Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative (Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1284-1289.1971
更新日期:1971-12-01 00:00:00
abstract::The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.9.2635-2638.2000
更新日期:2000-05-01 00:00:00
abstract::Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.516-519.1976
更新日期:1976-04-01 00:00:00
abstract::The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.6948-6957.1999
更新日期:1999-11-01 00:00:00
abstract::Streptomyces coelicolor is the prototype for the investigation of antibiotic-producing and differentiating actinomycetes. As soil bacteria, streptomycetes can metabolize a wide variety of carbon sources and are hence vested with various specific permeases. Their activity and regulation substantially determine the nutr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.5.1362-1373.2004
更新日期:2004-03-01 00:00:00
abstract::The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.1016-1024.1967
更新日期:1967-10-01 00:00:00
abstract::Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.13.3466-3475.2002
更新日期:2002-07-01 00:00:00
abstract::Margherita, S. S. (Temple University School of Medicine, Philadelphia, Pa.), and H. Friedmann. Induction of nonspecific resistance by endotoxin in unresponsive mice. J. Bacteriol. 89:277-280. 1965.-Serratia marcescens endotoxin was tested for protective effect in animals of varying immunological competence. A quantity...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.2.277-280.1965
更新日期:1965-02-01 00:00:00
abstract::A mutational change of the initiation codon to GUA was found to reduce, but not abolish, expression of the recJ gene of Escherichia coli. Specific mutations in translational initiation factor IF3 have been isolated as second-site suppressors of this GUA initiation codon mutation. One of these, infC135, with an arginin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.21.6705-6713.1997
更新日期:1997-11-01 00:00:00
abstract::The HPB12 protein from the nucleoid of Bacillus subtilis was previously described, and its DNA binding properties have been reported previously (V. Salti, F. Le Hégarat, and L. Hirschbein, Biochim. Biophys. Acta 1009:161-167, 1989). The DNA-HPB12 complexes were examined by electron microscopy. They appeared as short, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.1.50-60.1994
更新日期:1994-01-01 00:00:00
abstract::Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00718-18
更新日期:2019-05-08 00:00:00
abstract::The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1364-1373.2000
更新日期:2000-03-01 00:00:00
abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00969-07
更新日期:2007-12-01 00:00:00
abstract::A Synechocystis sp. strain PCC 6803 mutant lacking CtaI, a main subunit of cytochrome c oxidase, is not capable of growing at light intensities below 5 micromol photons m(-2) s(-1), presumably due to an overreduced plastoquinone pool in the thylakoid membrane. Upon selection for growth at light intensities below 5 mic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1286-1294.2006
更新日期:2006-02-01 00:00:00
abstract::The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.33-41.1976
更新日期:1976-01-01 00:00:00