Regulation of sialic acid metabolism in Escherichia coli: role of N-acylneuraminate pyruvate-lyase.

Abstract:

:In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in mutants defective in sialic acid polymerization and in CMP-NeuNAc synthesis. Polymerization-defective mutants could not synthesize the polysialic acid capsule and accumulated ca. 25-fold more CMP-NeuNAc than the wild type. Mutants unable to activate sialic acid because of a defect in CMP-NeuNAc synthetase accumulated ca. sevenfold more sialic acid than the wild type. An additional threefold increase in sialic acid levels occurred when a mutation resulting in loss of N-acylneuraminate pyruvate-lysase (sialic acid aldolase) was introduced into the CMP-NeuNAc synthetase-deficient mutant. The aldolase mutation could not be introduced into the polymerization-defective mutant, suggesting that any further increase in the intracellular CMP-NeuNAc concentration was toxic. These results show that sialic acid aldolase can regulate the intracellular concentration of sialic acid and therefore the concentration of CMP-NeuNAc. We conclude that regulation of aldolase, mediated by sialic acid induction, is necessary not only for dissimilating sialic acid (E.R. Vimr and F. A. Troy, J. Bacteriol. 164:845-853, 1985) but also for modulating the level of metabolic intermediates in the sialic acid pathway. In agreement with this conclusion, an increase in the intracellular sialic acid concentration was correlated with an increase in aldolase activity. Direct evidence for the central role of aldolase in regulating the metabolic flux of sialic adid in E. coli was provided by the finding that exogenous radiolabeled sialic acid was specifically incorporated into sialyl polymer in aldolase-negative strain but not in the wild type.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Vimr ER,Troy FA

doi

10.1128/JB.164.2.854-860.1985

subject

Has Abstract

pub_date

1985-11-01 00:00:00

pages

854-60

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

164

pub_type

杂志文章
  • Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps.

    abstract::Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01003-08

    authors: Toh E,Kurtz HD Jr,Brun YV

    更新日期:2008-11-01 00:00:00

  • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.

    abstract::Ruminococcin A (RumA) is a trypsin-dependent lantibiotic produced by Ruminococcus gnavus E1, a gram-positive strict anaerobic strain isolated from a human intestinal microbiota. A 12.8-kb region from R. gnavus E1 chromosome, containing the biosynthetic gene cluster of RumA, has been cloned and sequenced. It consisted ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.1.18-28.2002

    authors: Gomez A,Ladiré M,Marcille F,Fons M

    更新日期:2002-01-01 00:00:00

  • Characterization of stress-responsive behavior in Pseudomonas aeruginosa PAO: isolation of Tn3-lacZYA fusions with novel damage-inducible (din) promoters.

    abstract::Although the pervasive soil and water microorganism Pseudomonas aeruginosa demonstrates heightened sensitivity to UV radiation, this species possesses a recA gene that, based on structural and functional properties, could mediate a DNA damage-responsive regulon similar to the SOS regulon of Escherichia coli. To determ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.6.1862-1868.1992

    authors: Warner-Bartnicki AL,Miller RV

    更新日期:1992-03-01 00:00:00

  • Cloning and sequencing of a Bacteroides ruminicola B(1)4 endoglucanase gene.

    abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.7.3620-3630.1990

    authors: Matsushita O,Russell JB,Wilson DB

    更新日期:1990-07-01 00:00:00

  • Analysis of plasmid deletional instability in Bacillus subtilis.

    abstract::Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1014-1023.1985

    authors: Hahn J,Dubnau D

    更新日期:1985-06-01 00:00:00

  • Denitrification by Alcaligenes eutrophus is plasmid dependent.

    abstract::Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of convertin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.852-854.1985

    authors: Römermann D,Friedrich B

    更新日期:1985-05-01 00:00:00

  • Regulatory effect of hydrogen on nitrogenase activity of the blue-green alga (cyanobacterium) Nostoc muscorum.

    abstract::Preincubation of the blue-green alga (cyanobacterium) Nostoc muscorum under an atmosphere of argon plus acetylene in the light led to a greater than fourfold increase of light-induced hydrogen evolution and to a 50% increase of acetylene reduction, as compared to cells that had not been preconditioned. The basic and t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1037-1040.1980

    authors: Scherer S,Kerfin W,Böger P

    更新日期:1980-03-01 00:00:00

  • Ribulose diphosphate carboxylase from autotrophic microorganisms.

    abstract::Thiobacillus denitrificans was grown anaerobically with nitrate as an acceptor in both sterile and nonsterile media. Ribulose diphosphate carboxylase was stable throughout the exponential growth phase and declined slowly only after cells reached the stationary phase. Reversible inactivation of the carboxylase occurred...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.2.633-642.1972

    authors: McFadden BA,Denend AR

    更新日期:1972-05-01 00:00:00

  • An AlgU-Regulated Antisense Transcript Encoded within the Pseudomonas syringae fleQ Gene Has a Positive Effect on Motility.

    abstract::Production of bacterial flagella is controlled by a multitiered regulatory system that coordinates the expression of 40 to 50 subunits and ordered assembly of these elaborate structures. Flagellar expression is environmentally controlled, presumably to optimize the benefits and liabilities of having these organelles o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00576-17

    authors: Markel E,Dalenberg H,Monteil CL,Vinatzer BA,Swingle B

    更新日期:2018-03-12 00:00:00

  • Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: comparison with procaryotic and eucaryotic dihydrofolate reductases.

    abstract::We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1001-1008.1983

    authors: Simonsen CC,Chen EY,Levinson AD

    更新日期:1983-09-01 00:00:00

  • Escherichia coli Lrp (leucine-responsive regulatory protein) does not directly regulate expression of the leu operon promoter.

    abstract::Studies by R. Lin et al. (J. Bacteriol. 174:1948-1955, 1992) suggested that the Escherichia coli leu operon might be a member of the Lrp regulon. Their results were obtained with a leucine auxotroph; in leucine prototrophs grown in a medium lacking leucine, there was little difference in leu operon expression between ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6547-6551.1999

    authors: Landgraf JR,Boxer JA,Calvo JM

    更新日期:1999-10-01 00:00:00

  • Isolation and preliminary characterization of auxotrophs of a filamentous Cyanobacterium.

    abstract::Auxotrophic mutants of the filamentous cyanobacterium Anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. Auxotrophs were identified by their inability to grow on minimal medium, and they were partially char...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.3.1556-1562.1977

    authors: Currier TC,Haury JF,Wolk CP

    更新日期:1977-03-01 00:00:00

  • Draft genome sequence of probiotic strain Lactobacillus rhamnosus R0011.

    abstract::Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06584-11

    authors: Tompkins TA,Barreau G,de Carvalho VG

    更新日期:2012-02-01 00:00:00

  • Effects of H2 and formate on growth yield and regulation of methanogenesis in Methanococcus maripaludis.

    abstract::Hydrogenotrophic methanogenic Archaea are defined by an H2 requirement for growth. Despite this requirement, many hydrogenotrophs are also capable of growth with formate as an electron donor for methanogenesis. While certain responses of these organisms to hydrogen availability have been characterized, responses to fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02141-12

    authors: Costa KC,Yoon SH,Pan M,Burn JA,Baliga NS,Leigh JA

    更新日期:2013-04-01 00:00:00

  • Interference with murein turnover has no effect on growth but reduces beta-lactamase induction in Escherichia coli.

    abstract::Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.23.7192-7198.1999

    authors: Kraft AR,Prabhu J,Ursinus A,Höltje JV

    更新日期:1999-12-01 00:00:00

  • Role of lipid intermediate(s) in the synthesis of serogroup B Neisseria meningitidis capsular polysaccharide.

    abstract::Neisseria meningitidis serogroup B strain M986 was examined for the involvement of lipid intermediate(s) participating in the biosynthesis of the sialic acid capsular polysaccharide. The addition of exogenous undecaprenyl phosphate, phosphatidylethanolamine, or phosphatidylglycerol to particulate membranes, in the pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.861-867.1985

    authors: Masson L,Holbein BE

    更新日期:1985-03-01 00:00:00

  • Purification of the Escherichia coli purine regulon repressor and identification of corepressors.

    abstract::The Escherichia coli pur regulon repressor protein was overproduced in a phage T7 expression system. The overexpressed repressor constituted approximately 35% of the soluble cellular protein. Pur repressor was purified to near homogeneity by two chromatographic steps. Hypoxanthine or guanine was required for binding o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.10.5637-5642.1990

    authors: Rolfes RJ,Zalkin H

    更新日期:1990-10-01 00:00:00

  • Regulation of a common amidotransferase subunit.

    abstract::In Bacillus subtilis the trpX locus specifies a glutamine-binding protein designated subunit X, which forms a complex with subunit E to constitute the anthranilate synthase enzyme aggregate (EX) and subunit A to constitute the p-aminobenzoate synthase enzyme aggregate (AX). Subunit X confers upon these enzyme complexe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.419-425.1977

    authors: Kane JF

    更新日期:1977-11-01 00:00:00

  • Regulation of perR expression by iron and PerR in Campylobacter jejuni.

    abstract::Campylobacter jejuni is a leading food-borne pathogen causing gastroenteritis in humans. Although OxyR is a widespread oxidative stress regulator in many Gram-negative bacteria, C. jejuni lacks OxyR and instead possesses the metalloregulator PerR. Despite the important role played by PerR in oxidative stress defense, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05493-11

    authors: Kim M,Hwang S,Ryu S,Jeon B

    更新日期:2011-11-01 00:00:00

  • Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi.

    abstract::A thermosensitive episome bearing the transposon Tn10, F(Ts)::Tn10 Lac+, has been successfully transferred from Escherichia coli to several wild strains of the enterobacteria Erwinia carotovora subsp. chrysanthemi, which are pathogenic on Saintpaulia ionantha. In one of these strains, all of the characters controlled ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.1.122-131.1982

    authors: Kotoujansky A,Lemattre M,Boistard P

    更新日期:1982-04-01 00:00:00

  • Characterization of genes for synthesis and catabolism of a new rhizopine induced in nodules by Rhizobium meliloti Rm220-3: extension of the rhizopine concept.

    abstract::Rhizopines are selective growth substrates synthesized in nodules only by strains of rhizobia capable of their catabolism. We report the isolation and study of genes for the synthesis and catabolism of a new rhizopine, scyllo-inosamine (sIa), from alfalfa nodules induced by Rhizobium meliloti Rm220-3. This compound is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.16.5205-5215.1993

    authors: Saint CP,Wexler M,Murphy PJ,Tempé J,Tate ME,Murphy PJ

    更新日期:1993-08-01 00:00:00

  • Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus.

    abstract::A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1424-1428.1975

    authors: Novick R,Zouzias D,Rush M

    更新日期:1975-12-01 00:00:00

  • Draft genome sequence of Tsukamurella sp. strain 1534.

    abstract::A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01279-12

    authors: Oulmi L,Gorlas A,Gimenez G,Robert C,Boulahrouf A,Raoult D,Roux V

    更新日期:2012-10-01 00:00:00

  • Suppression of the defects in rdgB mutants of Escherichia coli K-12 by the cloned purA gene.

    abstract::A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.3.1360-1362.1991

    authors: Clyman J,Cunningham RP

    更新日期:1991-02-01 00:00:00

  • Deoxyribonucleic acid base composition of species in the yeast genus Kluyveromyces van der Walt emend. van der Walt.

    abstract::The deoxyribonucleic acid base composition (percent guanine + cytosine [GC]) was determined for 29 strains, representing 18 species of the genus Kluyveromyces. It was concluded that on the basis of GC content (47.4%) and other properties K. veronae occupies an uncertain position in the genus Kluyveromyces. The GC cont...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.481-487.1972

    authors: Martini A,Phaff HJ,Douglass SA

    更新日期:1972-08-01 00:00:00

  • The chromosomal response regulatory gene chvI of Agrobacterium tumefaciens complements an Escherichia coli phoB mutation and is required for virulence.

    abstract::In an effort to identify the Agrobacterium tumefaciens phosphate regulatory gene(s), we isolated a clone from an A. tumefaciens cosmid library that restored regulated alkaline phosphatase activity to an Escherichia coli phoB mutant. The gene that complemented phoB was localized by subcloning and deletion analysis, and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.20.6626-6636.1993

    authors: Mantis NJ,Winans SC

    更新日期:1993-10-01 00:00:00

  • Complete genome sequence of Listeria seeligeri, a nonpathogenic member of the genus Listeria.

    abstract::We report the complete and annotated genome sequence of the nonpathogenic Listeria seeligeri SLCC3954 serovar 1/2b type strain harboring the smallest completely sequenced genome of the genus Listeria. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01415-09

    authors: Steinweg C,Kuenne CT,Billion A,Mraheil MA,Domann E,Ghai R,Barbuddhe SB,Kärst U,Goesmann A,Pühler A,Weisshaar B,Wehland J,Lampidis R,Kreft J,Goebel W,Chakraborty T,Hain T

    更新日期:2010-03-01 00:00:00

  • Role of genetic redundancy in polyhydroxyalkanoate (PHA) polymerases in PHA biosynthesis in Rhodospirillum rubrum.

    abstract::This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01111-12

    authors: Jin H,Nikolau BJ

    更新日期:2012-10-01 00:00:00

  • Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli.

    abstract::Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4945-4952.1989

    authors: Pickett CL,Twiddy EM,Coker C,Holmes RK

    更新日期:1989-09-01 00:00:00

  • Effect of glutamine on enzymes of nitrogen metabolism in Bacillus subtilis.

    abstract::An earlier study of the regulation of glutamate synthase (GOGAT) in Bacillus subtilis (Deshpande et al., Bichem. Biophys. Res. Commun. 95:55--60, 1980) revealed an inverse relationship between the specific activity of this essential ammonia-assimilatory enzyme and the intracellular pool of glutamine: GOGAT activity de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.768-774.1981

    authors: Deshpande KL,Katze JR,Kane JF

    更新日期:1981-02-01 00:00:00