Suppressor of deoxythmidine monophosphate uptake in Saccharomyces cerevisiae.

Abstract:

:Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium. The sot1 mutation maps between rad1 and the centromere of chromosome XVI, and is unlinked to any of the tup mutations. The sot1 mutation does not suppress the other pleiotropic effects of the tup1 mutant, notably the lack of mating of tup1 MATalpha strains. The sot1 mutation specifically blocks the uptake of dTMP into tup strains. After growing a sot1 strain in medium containing [3H]dTMP, we showed that the medium still contained more than 90% of the original [3H]dTMP and that this medium could support the incorporation of [3H]dTMP by a tup2 strain. Therefore, sot1 strains do not degrade dTMP in the medium. The sot1 mutation had no effect on the uptake of other nutrients essential for growth, including several amino acids, adenine, and uracil.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Remer S,Sherman A,Kraig E,Haber JE

doi

10.1128/JB.138.2.638-641.1979

subject

Has Abstract

pub_date

1979-05-01 00:00:00

pages

638-41

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

138

pub_type

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