Control of the kilA gene of the broad-host-range plasmid RK2: involvement of korA, korB, and a new gene, korE.

Abstract:

:Broad-host-range plasmid RK2 encodes several different kil genes which are potentially lethal to an Escherichia coli host. The kil genes and the essential RK2 replication gene trfA are regulated by the products of kor genes. We have shown previously that kilA can be controlled by a constitutively expressed korA gene. In this study, we have found that the wild-type, autoregulated korA gene is insufficient for control of kilA cloned on high-copy-number plasmids. One of two other genes must also be present with korA. One gene is korB, originally discovered by its ability to control the determinants in the kilB region and later found to affect expression of both trfA and korA. The other is a new gene, korE, which has been cloned from the 2.2' to 4.1' region located between korC and kilA. Studies with a kilA-cat fusion suggest that korA, korB, and korE all participate in the control of kilA gene expression.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Young C,Burlage RS,Figurski DH

doi

10.1128/jb.169.3.1315-1320.1987

subject

Has Abstract

pub_date

1987-03-01 00:00:00

pages

1315-20

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

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