Isolation and characterization of a selenium metabolism mutant of Salmonella typhimurium.

abstract：:Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for g...

journal_title：Journal of bacteriology

authors： Magill CW,Sabina RL,Garber TL,Magill JM

更新日期：1982-03-01 00:00:00

abstract：:DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different ...

journal_title：Journal of bacteriology

authors： Cann IK,Ishino S,Nomura N,Sako Y,Ishino Y

更新日期：1999-10-01 00:00:00

abstract：:plsB mutants of Escherichia coli are sn-glycerol 3-phosphate auxotrophs which owe their requirement to a K(m) defect in sn-glycerol 3-phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. We have located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene de...

journal_title：Journal of bacteriology

authors： Cronan JE Jr,Bell RM

更新日期：1974-10-01 00:00:00

abstract：:Bacitracin is a peptide antibiotic nonribosomally produced by Bacillus licheniformis. The bcrABC genes which confer bacitracin resistance to the bacitracin producer encode ATP binding cassette (ABC) transporter proteins, which are hypothesized to pump out bacitracin from the cells. Bacillus subtilis 168, which has no ...

journal_title：Journal of bacteriology

authors： Ohki R,Tateno K,Okada Y,Okajima H,Asai K,Sadaie Y,Murata M,Aiso T

更新日期：2003-01-01 00:00:00

abstract：:In Streptococcus mutans, enzyme II(scr) and sucrose-6-phosphate hydrolase are two important enzymes in the transport and metabolism of dietary sucrose. The scr regulon of S. mutans is composed of three genes, scrA and scrB, which code for enzyme II(scr) and sucrose-6-phosphate hydrolase, respectively, and scrR, which ...

journal_title：Journal of bacteriology

authors： Wang B,Kuramitsu HK

更新日期：2003-10-01 00:00:00

abstract：:Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...

journal_title：Journal of bacteriology

authors： Jensen KK,Sharkova E,Duggan MF,Qi Y,Koide A,Hoch JA,Hulett FM

更新日期：1993-06-01 00:00:00

abstract：:Penicillin-binding protein (PBP) 7 of Escherichia coli is a poorly characterized member of the family of enzymes that synthesize and modify the bacterial cell wall. The approximate chromosomal position of the gene encoding this protein was determined by measuring the expression of PBPs during lytic infection of E. col...

journal_title：Journal of bacteriology

authors： Henderson TA,Templin M,Young KD

更新日期：1995-04-01 00:00:00

abstract：:Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...

journal_title：Journal of bacteriology

authors： Tsuboi A,Uchihi R,Tabata R,Takahashi Y,Hashiba H,Sasaki T,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1986-10-01 00:00:00

abstract：:Cells colicinogenic for the colicin plasmids E1 or E2 (Col E1 and Col E2, respectively) were selected for a loss of colicin production after infection with bacteriophage Mu. Extrachromosomal deoxyribonucleic acid that was larger than the original colicin plasmids was found in such cells. A small insertion mutant in Co...

journal_title：Journal of bacteriology

authors： Inselburg J

更新日期：1974-08-01 00:00:00

abstract：:The extent and nature of DNA polymorphism in the mutS-rpoS region of the Escherichia coli genome were assessed in 21 strains of enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) and in 6 strains originally isolated from natural populations. The intervening region between mutS and rpoS was amplified ...

journal_title：Journal of bacteriology

authors： Herbelin CJ,Chirillo SC,Melnick KA,Whittam TS

更新日期：2000-10-01 00:00:00

abstract：:Eighty-seven different enterobacteria and pseudomonas strains were examined for the presence of extrachromosomal deoxyribonucleic acid (DNA). Thirty-four strains contained closed circular DNA by the ethidium bromide CsCl density technique. Extrachromosomal DNA was most frequent in Escherichia and Klebsiella strains. T...

journal_title：Journal of bacteriology

authors： Christiansen C,Christiansen G,Bak AL,Stenderup A

更新日期：1973-04-01 00:00:00

abstract：:The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning the gene behind the l...

journal_title：Journal of bacteriology

authors： Talarico TL,Ray PH,Dev IK,Merrill BM,Dallas WS

更新日期：1992-09-01 00:00:00

abstract：:A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

journal_title：Journal of bacteriology

authors： Young CS,Beatty JT

更新日期：1998-09-01 00:00:00

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:Expression of the 146-kilodalton (kDa) extracellular glucoamylase by the budding yeast Schwanniomyces castellii is induced by maltose and starch. By use of antiglucoamylase antisera, we found that this expression was regulated at the level of the mRNA, taking place within 30 min after exposure of yeast cells to the re...

journal_title：Journal of bacteriology

authors： Dowhanick TM,Russell I,Scherer SW,Stewart GG,Seligy VL

更新日期：1990-05-01 00:00:00

abstract：:The cell wall constituents of Leuconostoc citrovorum 8082, L. mesenteroides 10830a, and L. mesenteroides 11449 have been ascertained. All three strains contained glycerol. Glucose and rhamnose were the major reducing sugar constituents. Alanine, glutamic acid, lysine, glucosamine, and muramic acid were the principal a...

journal_title：Journal of bacteriology

authors： Harney SJ,Simopoulos ND,Ikawa M

更新日期：1967-01-01 00:00:00

abstract：:To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a c...

journal_title：Journal of bacteriology

authors： Lamont I,Richardson H,Carter DR,Egan JB

更新日期：1993-08-01 00:00:00

abstract：:The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

journal_title：Journal of bacteriology

authors： Peng JB,Yan WM,Bao XZ

更新日期：1994-05-01 00:00:00

abstract：:Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. baumannii, we determined the complete genome sequence of A. baumannii strain 1656-2, which forms sturdy biofilm and is resistant to multiple drugs. ...

journal_title：Journal of bacteriology

authors： Park JY,Kim S,Kim SM,Cha SH,Lim SK,Kim J

更新日期：2011-11-01 00:00:00

abstract：:In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode,...

journal_title：Journal of bacteriology

authors： Smith DW,Boerner P

更新日期：1975-04-01 00:00:00

abstract：:SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, an...

journal_title：Journal of bacteriology

authors： Balasubramanian R,Shen G,Bryant DA,Golbeck JH

更新日期：2006-05-01 00:00:00

abstract：:Cytochrome c' (Cyt c') is a c-type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in Rhodobacter sphaeroides 2.4.3, designated cycP, was isolated and sequenced. Northern blot analysis and beta-galactosidase assays demonstrated that cycP transcription increased as oxygen levels decreased an...

journal_title：Journal of bacteriology

authors： Choi PS,Grigoryants VM,Abruña HD,Scholes CP,Shapleigh JP

更新日期：2005-06-01 00:00:00

abstract：:ermC specifies an rRNA methyltransferase that confers resistance to erythromycin. The expression of this determinant is induced by the addition of erythromycin. The induction mechanism has been shown to operate posttranscriptionally, and its mechanism has been elucidated. We now show that synthesis of the ermC gene pr...

journal_title：Journal of bacteriology

authors： Denoya CD,Bechhofer DH,Dubnau D

更新日期：1986-12-01 00:00:00

abstract：:After the demonstration that Stigmatella aurantiaca DW4 secretes an endo-N-acetyl-beta-D-glucosaminidase (ENGase), acting on the di-N-acetylchitobiosyl part of N-linked glycans (S. Bourgerie, Y. Karamanos, T. Grard, and R. Julien, J. Bacteriol. 176:6170-6174, 1994), an ENGase activity having the same substrate specifi...

journal_title：Journal of bacteriology

authors： Barreaud JP,Bourgerie S,Julien R,Guespin-Michel JF,Karamanos Y

更新日期：1995-02-01 00:00:00

abstract：:Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

journal_title：Journal of bacteriology

authors： Weickert MJ,Adhya S

更新日期：1993-01-01 00:00:00

abstract：:The cold-tolerant bacterium Pusillimonas sp. strain T7-7 is able to utilize diesel oils (C5 to C30 alkanes) as a sole carbon and energy source. In the present study, bioinformatics, proteomics, and real-time reverse transcriptase PCR approaches were used to identify the alkane hydroxylation system present in this bact...

journal_title：Journal of bacteriology

authors： Li P,Wang L,Feng L

更新日期：2013-05-01 00:00:00

abstract：:In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into th...

journal_title：Journal of bacteriology

authors： Keenleyside WJ,Bronner D,Jann K,Jann B,Whitfield C

更新日期：1993-10-01 00:00:00

abstract：:Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

journal_title：Journal of bacteriology

authors： Goldman BS,Lin JT,Stewart V

更新日期：1994-08-01 00:00:00

abstract：:DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active ...

journal_title：Journal of bacteriology

authors： Brasch MA,Meyer RJ

更新日期：1986-08-01 00:00:00

abstract：:Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose int...

journal_title：Journal of bacteriology

authors： Richardson JS,Carpena X,Switala J,Perez-Luque R,Donald LJ,Loewen PC,Oresnik IJ

更新日期：2008-04-01 00:00:00