Abstract:
:DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and aphidicolin-resistant (fraction II) activities were detected. The activity in fraction I was more heat stable than that in fraction II. Two different genes (polA and polB) encoding family B DNA polymerases were cloned from the organism by PCR using degenerated primers based on the two conserved motifs (motif A and B). The deduced amino acid sequences from their entire coding regions contained all of the motifs identified in family B DNA polymerases for 3'-->5' exonuclease and polymerase activities. The product of polA gene (Pol I) was aphidicolin resistant and heat stable up to 80 degrees C. In contrast, the product of polB gene (Pol II) was aphidicolin sensitive and stable at 95 degrees C. These properties of Pol I and Pol II are similar to those of fractions II and I, respectively, and moreover, those of Pol I and Pol II of Pyrodictium occultum. The deduced amino acid sequence of A. pernix Pol I exhibited the highest identities to archaeal family B DNA polymerase homologs found only in the crenarchaeotes (group I), while Pol II exhibited identities to homologs found in both euryarchaeotes and crenarchaeotes (group II). These results provide further evidence that the subdomain Crenarchaeota has two family B DNA polymerases. Furthermore, at least two DNA polymerases work in the crenarchaeal cells, as found in euryarchaeotes, which contain one family B DNA polymerase and one heterodimeric DNA polymerase of a novel family.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cann IK,Ishino S,Nomura N,Sako Y,Ishino Ydoi
10.1128/JB.181.19.5984-5992.1999keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
5984-92issue
19eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1731-1736.1987
更新日期:1987-04-01 00:00:00
abstract::Deletions in the rpoBC region have been transferred to phage lambda and characterized in detail by genetic, structural, and functional tests. We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.479-489.1979
更新日期:1979-11-01 00:00:00
abstract::Escherichia coli B/r ATCC 12407 which grew synchronously with interdivision times of 45 and 85 min were exposed at intervals to chloramphenicol (200 mug/ml) and [(14)C]thymidine for short or long (residual) periods. The results suggested that the pattern of residual thymidine incorporation in 85-min cells yielded an e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.565-567.1974
更新日期:1974-10-01 00:00:00
abstract::Coxiella burnetii, the etiological agent of acute and chronic Q fever in humans, is a naturally intracellular pathogen that directs the formation of an acidic Coxiella-containing vacuole (CCV) derived from the host lysosomal network. Central to its pathogenesis is a specialized type IVB secretion system (T4SS) that de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00071-13
更新日期:2013-09-01 00:00:00
abstract::The group A Streptococcus (GAS) sof gene encodes the serum opacity factor protein, which is capable of opacifying mammalian sera and binding at least two host proteins, fibronectin and fibrinogen. The sof gene exists in approximately 50% of clinical isolates, and there is a classical association of so-called nephritog...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.4.1208-1217.2003
更新日期:2003-02-01 00:00:00
abstract::The mutant am-14 produces no active nicotinamide adenine dinucleotide phosphate-linked glutamate dehydrogenase (GDH) and no protein showing immunological cross-reaction with the enzyme. Nevertheless, it shows complementation with several other am mutants in heterokaryons. Active GDH can be extracted from heterokaryons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.3.787-792.1968
更新日期:1968-03-01 00:00:00
abstract::Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. Tetracycline-sensitive point mutations were mapped in recombination tests with the deletions and were thus a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.2.921-929.1983
更新日期:1983-02-01 00:00:00
abstract::Toxin-antitoxin (TA) loci consist of two genes in an operon, encoding a stable toxin and an unstable antitoxin. The expression of toxin leads to cell growth arrest and sometimes bacterial death, while the antitoxin prevents the cytotoxic activity of the toxin. In this study, we show that the chromosome of Yersinia pes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00336-10
更新日期:2010-07-01 00:00:00
abstract::Members of Thermococcales harbor a number of genes encoding putative aminotransferase Class III enzymes. Here, we characterized the TK1211 protein from the hyperthermophilic archaeon Thermococcus kodakarensis The TK1211 gene was expressed in T. kodakarensis under the control of the strong, constitutive promoter of the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00617-20
更新日期:2021-01-19 00:00:00
abstract::Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01412-08
更新日期:2009-04-01 00:00:00
abstract::pdrK encodes a pyridoxine (PN)/pyridoxal (PL)/pyridoxamine (PM) kinase thought to function in the salvage pathway of pyridoxal 5'-phosphate (PLP) coenzyme biosynthesis. The observation that pdxK null mutants still contain PL kinase activity led to the hypothesis that Escherichia coli K-12 contains at least one other B...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.7.1814-1821.1998
更新日期:1998-04-01 00:00:00
abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1621-1623.1975
更新日期:1975-12-01 00:00:00
abstract::Production of bacterial flagella is controlled by a multitiered regulatory system that coordinates the expression of 40 to 50 subunits and ordered assembly of these elaborate structures. Flagellar expression is environmentally controlled, presumably to optimize the benefits and liabilities of having these organelles o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00576-17
更新日期:2018-03-12 00:00:00
abstract::Expression of the pneumococcal type 1 pilus is bistable and positively regulated by the transcription factor RlrA. RlrA is also known to positively control its own expression. Here we present evidence that bistable expression of the type 1 pilus is mediated by the positive-feedback loop controlling rlrA expression. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06078-11
更新日期:2012-03-01 00:00:00
abstract::Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.184-191.1967
更新日期:1967-07-01 00:00:00
abstract::Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.156-160.1969
更新日期:1969-07-01 00:00:00
abstract::Necrotic enteritis (NE) is an economically important disease of poultry caused by certain Clostridium perfringens type A strains. NE pathogenesis involves the NetB toxin, which is encoded on a large conjugative plasmid within a 42-kb pathogenicity locus. Recent multilocus sequence type (MLST) studies have identified t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01032-12
更新日期:2013-03-01 00:00:00
abstract::Methanohalophilus strain FDF1 synthesizes beta-glutamine, betaine, and N epsilon-acetyl-beta-lysine as osmoprotective agents when the cells are grown in high external concentrations of NaCl. Nuclear magnetic resonance spectroscopic analyses of 13CH3OH-12CO2 label incorporation by the cells provide information on the b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.20.6688-6693.1992
更新日期:1992-10-01 00:00:00
abstract::Activities of gamma-butyrobetaine hydroxylase and carnitine dehydrogenase were low in cells of Pseudomonas sp. AK 1 grown in the absence of their respective substrates. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.3.1094-1095.1970
更新日期:1970-03-01 00:00:00
abstract::We have developed a cytoduction assay to measure cell fusion quantitatively in the basidiomycete corn smut fungus Ustilago maydis. This assay employs a mutation conferring resistance to oligomycin that exhibits non-Mendelian inheritance and presumably affects the mitochondrial genome. After auxotrophic olir cells are ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.23.7831-7833.1992
更新日期:1992-12-01 00:00:00
abstract::Level of competence reached by Bacillus subtilis 168 lysogenic for temperate phage phi 105 was reduced compared to that reached by nonlysogenic cells. This effect was probably related to an alteration of the bacterial surface. Deoxyribonucleic acid extracted from phi 105 lysogenic bacteria was used to transform other ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.874-877.1969
更新日期:1969-06-01 00:00:00
abstract::Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.708-714.1986
更新日期:1986-11-01 00:00:00
abstract::The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00267-17
更新日期:2017-08-08 00:00:00
abstract::The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.22.7659-7669.2004
更新日期:2004-11-01 00:00:00
abstract::The bdr (Borrelia direct repeat) gene family of the genus Borrelia encodes a polymorphic group of proteins that carry a central repeat motif region containing putative phosphorylation sites and a hydrophobic carboxyl-terminal domain. It has been postulated that the Bdr proteins may anchor to the inner membrane via the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.15.4222-4226.2000
更新日期:2000-08-01 00:00:00
abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.125-135.1976
更新日期:1976-01-01 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::RecAX53 is a chimeric variant of the Escherichia coli RecA protein (RecAEc) that contains a part of the central domain of Pseudomonas aeruginosa RecA (RecAPa), encompassing a region that differs from RecAEc at 12 amino acid positions. Like RecAPa, this chimera exhibits hyperrecombination activity in E. coli cells, inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01006-07
更新日期:2008-04-01 00:00:00
abstract::Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.5.1296-1303.2000
更新日期:2000-03-01 00:00:00
abstract::The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.4970-4978.1993
更新日期:1993-08-01 00:00:00