Competitive inhibition of transformation in group H Streptococcus strain Challis by heterologous deoxyribonucleic acid.

abstract：:The Bacillus subtilis anti-TRAP protein regulates the ability of the tryptophan-activated TRAP protein to bind to trp operon leader RNA and promote transcription termination. AT synthesis is regulated both transcriptionally and translationally by uncharged tRNA(Trp). In this study, we examined the roles of AT synthesi...

journal_title：Journal of bacteriology

authors： Cruz-Vera LR,Gong M,Yanofsky C

更新日期：2008-03-01 00:00:00

abstract：:DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active ...

journal_title：Journal of bacteriology

authors： Brasch MA,Meyer RJ

更新日期：1986-08-01 00:00:00

abstract：:The first alkaline phosphatase (APase) structural gene mutant of Bacillus subtilis 168 was constructed by using a clone identified by hybridization to a synthetic degenerative oligonucleotide. The design of the probe was based on the first 29 amino acids of the sequenced mature APase III protein, which had been isolat...

journal_title：Journal of bacteriology

authors： Bookstein C,Edwards CW,Kapp NV,Hulett FM

更新日期：1990-07-01 00:00:00

abstract：:Eisenstein, Richard B. (Western Reserve University, Cleveland, Ohio) and Charles Yanofsky. Tryptophan synthetase levels in Escherichia coli, Shigella dysenteriae, and transduction hybrids. J. Bacteriol. 83:193-204. 1962-Shigella dysenteriae and Escherichia coli, strains K-12 and B, were found to produce low levels of ...

journal_title：Journal of bacteriology

authors： EISENSTEIN RB,YANOFSKY C

更新日期：1962-01-01 00:00:00

abstract：:A Rhodobacter capsulatus hemC mutant has been isolated and used to show that oxygen regulates the intracellular levels of porphobilinogen. Experiments using a hemB-cat gene fusion demonstrated that oxygen does not transcriptionally regulate hemB transcription. Porphobilinogen synthase activity is not regulated by oxyg...

journal_title：Journal of bacteriology

authors： Biel AJ,Canada K,Huang D,Indest K,Sullivan K

更新日期：2002-03-01 00:00:00

abstract：:We have investigated the regulation of the operon encoding xylose utilization in Staphylococcus xylosus C2a and Staphylococcus carnosus TM300. For in vivo studies, transcriptional fusions of the xylAB regulatory region to the lipase gene from Staphylococcus hyicus were constructed. Repression of lipase activity depend...

journal_title：Journal of bacteriology

authors： Sizemore C,Wieland B,Götz F,Hillen W

更新日期：1992-05-01 00:00:00

abstract：:The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique protein...

journal_title：Journal of bacteriology

authors： Chromy BA,Choi MW,Murphy GA,Gonzales AD,Corzett CH,Chang BC,Fitch JP,McCutchen-Maloney SL

更新日期：2005-12-01 00:00:00

abstract：:The hutC gene in Pseudomonas putida encodes a repressor protein that negatively regulates the expression of all hut genes. We have overexpressed this cloned hutC gene in Escherichia coli to identify P. putida hut regions that could specifically bind the repressor. Ten restriction fragments, some of which were partiall...

journal_title：Journal of bacteriology

authors： Hu L,Allison SL,Phillips AT

更新日期：1989-08-01 00:00:00

abstract：UNLABELLED:An ability to sense and respond to changes in extracellular phosphate is critical for the survival of most bacteria. For Caulobacter crescentus, which typically lives in phosphate-limited environments, this process is especially crucial. Like many bacteria, Caulobacter responds to phosphate limitation throug...

journal_title：Journal of bacteriology

authors： Lubin EA,Henry JT,Fiebig A,Crosson S,Laub MT

更新日期：2015-10-19 00:00:00

abstract：:Heterokaryons of Mucor racemosus were produced by fusion of spheroplasts from two auxotrophic strains of the fungus. Germinated sporangiospores were converted to spheroplasts by using commercial chitinase and Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed in a buffered Ca(NO3)2 solut...

journal_title：Journal of bacteriology

authors： Genthner FJ,Borgia PT

更新日期：1978-04-01 00:00:00

abstract：:Several preparations of staphylococcal alpha-toxin and delta-lysin were studied in order to compare hemolytic activity with capacity to lyse bacterial protoplasts. delta-Lysin in relatively low concentration lysed protoplasts of Sarcina lutea, protoplasts of Streptococcus faecalis, and spheroplasts of Escherichia coli...

journal_title：Journal of bacteriology

authors： Bernheimer AW,Avigad LS,Grushoff P

更新日期：1968-08-01 00:00:00

abstract：:Evolutionary trajectories and mutational landscapes of drug-resistant bacteria are influenced by cell-intrinsic and extrinsic factors. In this study, I demonstrated that loss of the Lon protease altered susceptibility of Escherichia coli to trimethoprim and that these effects were strongly contingent on the drug conce...

journal_title：Journal of bacteriology

authors： Matange N

更新日期：2020-01-15 00:00:00

abstract：:Strains monosomic for chromosome I of Saccharomyces cerevisiae contain 25 to 35% fewer rRNA genes than do normal diploid strains. When these strains are repeatedly subcultured, colonies are isolated that have magnified their number of rRNA genes to the diploid amount while remaining monosomic for chromosome I. We have...

journal_title：Journal of bacteriology

authors： Kaback DB,Halvorson HO

更新日期：1978-04-01 00:00:00

abstract：:The rate at which the peptidoglycan precursor meso-diaminopimelic acid (DAP) is incorporated into the cell wall of Escherichia coli cells was determined by pulse-label experiments. For different E. coli strains, the incorporation rate was compared with the rate of uptake of DAP into the cell. With E. coli W7, a dap ly...

journal_title：Journal of bacteriology

authors： Wientjes FB,Pas E,Taschner PE,Woldringh CL

更新日期：1985-10-01 00:00:00

abstract：:Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...

journal_title：Journal of bacteriology

authors： Jürgens UJ,Weckesser J

更新日期：1985-10-01 00:00:00

abstract：:Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...

journal_title：Journal of bacteriology

authors： Buckner CM,Schyns G,Moran CP Jr

更新日期：1998-07-01 00:00:00

abstract：:The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increa...

journal_title：Journal of bacteriology

authors： Kajitani M,Kato A,Wada A,Inokuchi Y,Ishihama A

更新日期：1994-01-01 00:00:00

abstract：:Globomycin is an antibiotic which inhibits the processing of the prolipoprotein. Eighty globomycin-resistant mutants were independently isolated from Escherichia coli K-12 which had a deletion mutation in chromosomal lipoprotein gene (lpp), but contained a plasmid carrying the wild-type lpp gene. Twenty-six of the mut...

journal_title：Journal of bacteriology

authors： Zwiebel LJ,Inukai M,Nakamura K,Inouye M

更新日期：1981-01-01 00:00:00

abstract：:A hybrid plasmid was constructed between the 5.3-megadalton plasmid (pUH24) of Anacystis nidulans R2 and the Escherichia coli plasmid pBR322. This was accomplished by adding a transposon to pBR322 and transforming this DNA into A. nidulans. One resultant hybrid, pLS103, had a molecular weight of 6.8 x 10(6), replicate...

journal_title：Journal of bacteriology

authors： Sherman LA,van de Putte P

更新日期：1982-04-01 00:00:00

abstract：:Escherichia coli K-12 deficient in galactose-1-phosphate uridyl transferase is capable of converting significant amounts of d-[1-(14)C]galactose to (14)CO(2), whereas strains deficient in other enzymes of the Leloir pathway cannot do so. ...

journal_title：Journal of bacteriology

authors： LaPolla RJ,Geier MR,Friedman TB,Merril CR

更新日期：1975-10-01 00:00:00

abstract：:Studies of gene expression using fusions to lacZ demonstrated that the Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is in an operon with two downstream genes, spmA and spmB. Mutations affecting any one of these three genes resulted in the production of spores with reduced heat resistance. The c...

journal_title：Journal of bacteriology

authors： Popham DL,Illades-Aguiar B,Setlow P

更新日期：1995-08-01 00:00:00

abstract：:The complex flagella of Rhizobium meliloti 2011 and MVII-1 were analyzed with regard to serology, fine structure, subunits, and amino acid composition. The serological identities of flagellar filaments of the two strains were demonstrated by double immunodiffusion with antiflagellin antiserum. The filaments had a diam...

journal_title：Journal of bacteriology

authors： Krupski G,Götz R,Ober K,Pleier E,Schmitt R

更新日期：1985-04-01 00:00:00

abstract：:Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...

journal_title：Journal of bacteriology

authors： Veluthambi K,Krishnan M,Gould JH,Smith RH,Gelvin SB

更新日期：1989-07-01 00:00:00

abstract：:Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD...

journal_title：Journal of bacteriology

authors： Daws TD,Fuchs JA

更新日期：1984-02-01 00:00:00

abstract：:An early step in crown gall tumor formation involves the attachment of Agrobacterium tumefaciens to host plant cells. A. tumefaciens C58::A205 (C58 attR) is a Tn3HoHo1 insertion mutant that was found to be avirulent on Bryophyllum daigremontiana and unable to attach to carrot suspension cells. The mutation mapped to a...

journal_title：Journal of bacteriology

authors： Reuhs BL,Kim JS,Matthysse AG

更新日期：1997-09-01 00:00:00

abstract：:The A-subunit polypeptides of Shiga toxin, the Shiga-like toxins (SLTs), and the plant lectin ricin inactivate eucaryotic ribosomes by enzymatically depurinating 28S rRNA. Comparison of the amino acid sequences of the members of the Shiga toxin family and ricin revealed two regions of significant homology that lie wit...

journal_title：Journal of bacteriology

authors： Jackson MP,Deresiewicz RL,Calderwood SB

更新日期：1990-06-01 00:00:00

abstract：:The nucleotide sequences of a 3,479-base-pair HindIII DNA fragment from Bacillus sphaericus 2362 and a 2,940-base-pair fragment from strain 2297 were determined; only minor differences were detected between them. Each contained two open reading frames coding for proteins of 51.4 and 41.9 kilodaltons. Both proteins wer...

journal_title：Journal of bacteriology

authors： Baumann L,Broadwell AH,Baumann P

更新日期：1988-05-01 00:00:00

abstract：:Clinical isolates of the fungal respiratory and systemic pathogen Histoplasma capsulatum have been placed in several different classes by using genomic restriction fragment length polymorphisms (RFLPs), but in general have not been distinguished further. We report here that a polymerase chain reaction (PCR)-based DNA ...

journal_title：Journal of bacteriology

authors： Kersulyte D,Woods JP,Keath EJ,Goldman WE,Berg DE

更新日期：1992-11-01 00:00:00

abstract：:The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is lo...

journal_title：Journal of bacteriology

authors： Huang KJ,Schieberl JL,Igo MM

更新日期：1994-03-01 00:00:00

abstract：:Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose int...

journal_title：Journal of bacteriology

authors： Richardson JS,Carpena X,Switala J,Perez-Luque R,Donald LJ,Loewen PC,Oresnik IJ

更新日期：2008-04-01 00:00:00